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Invitrogen
IBA1 Recombinant Rabbit Monoclonal Antibody (145)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
IBA1 Antibody (MA5-29013) in WB
Western blot was performed using Anti-IBA1 Recombinant Rabbit Monoclonal Antibody (145) (Product # MA5-29013) and a 16 kDa band corresponding to Allograft inflammatory factor 1 was observed across positive cell lines (THP-1 and HL-60) and positive tissues (Mouse spleen and Mouse thymus tested) tested. Also, a band was observed in U937 treated with Retinoic acid (10 uM, 24 hours) treatment, showing upregulation of IBA1. Membrane enriched extracts (30 µg lysate) of U-937 (Lane 1), U-937 treated with Retinoic acid (Lane 2), HL-60 (Lane 3), TH... View More
Product Details
MA5-29013
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
145
Immunogen
Recombinant Human IBA1/AIF1 Protein (Met1-Pro147)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2784951
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human IBA1/AIF1.
Target Information
AIF1 is induced by cytokines and interferon. It is thought to be involved in the negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AIF-1; Allograft inflammatory factor 1; DADB-70P7.8; DASS-82G15.2; Em:AF129756.17; Iba; interferon gamma responsive transcript; ionized calcium binding adapter molecule 1; Ionized calcium-binding adapter molecule 1; Protein G1; testis specific
Gene Aliases: AI607846; AIF-1; AIF1; D17H6S50E; G1; IBA1; IRT-1; IRT1
UniProt ID: (Human) P55008, (Mouse) O70200
Entrez Gene ID: (Human) 199, (Mouse) 11629
microglial cell activation
positive regulation of protein phosphorylation
phagocytosis, engulfment
inflammatory response
negative regulation of gene expression
positive regulation of muscle hyperplasia
Rac protein signal transduction
actin filament polymerization
cellular response to extracellular stimulus
cellular response to hormone stimulus
positive regulation of T cell proliferation
negative regulation of apoptotic process
positive regulation of nitric oxide biosynthetic process
positive regulation of smooth muscle cell proliferation
negative regulation of smooth muscle cell proliferation
response to axon injury
actin filament bundle assembly
response to glucocorticoid
response to electrical stimulus
cellular response to morphine
cellular response to interferon-gamma
cellular response to hydroperoxide
negative regulation of smooth muscle cell chemotaxis
positive regulation of smooth muscle cell chemotaxis
positive regulation of monocyte chemotaxis
ruffle assembly
positive regulation of G1/S transition of mitotic cell cycle
positive regulation of T cell migration
positive regulation of cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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