Invitrogen

IBA1 Monoclonal Antibody (GT10312)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

13 Published Figures

22 References

View all (53) IBA1 antibodies

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Datasheet

Protocols

Questions & Answers

IBA1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 29

IBA1 Antibody (MA5-27726) in ICC/IF

IBA1 Monoclonal Antibody (GT10312) detects Iba1 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Iba1 protein stained by IBA1 Monoclonal Antibody (GT10312) (Product # MA5-27726) diluted at 1:200. Blue: Hoechst 33342 staining.

IBA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IBA1 Antibody in Immunohistochemistry (IHC)

IBA1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

IBA1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of IBA1 was observed in THP-1 in comparison to A-431, HeLa and MCF7 using Anti-IBA1 Monoclonal Antibody (GT10312) (Product # MA5-27726) in Western Blot. {RE}

Product Details

MA5-27726

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Flow Cytometry (Flow)

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT10312

Immunogen

Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735228

Product Specific Information

Positive Control: THP-1, HL-60

Predicted Reactivity: Pig (93%), Rhesus Monkey (99%), Bovine (88%)

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Target Information

AIF1 is induced by cytokines and interferon. It is thought to be involved in the negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The effect of laser radiation on glial fibrillary acidic protein and allograft inflammatory factor-1 expression in severed and surgically repaired sciatic nerve.

International journal of physiology, pathophysiology and pharmacology

Yashchyshyn Z,Kozyk M,Strubchevska K,Ziablitsev S

MA5-27726 was used in Western Blotting to determine the effect of different spectrum laser radiations on the expression of Glial Fibrillary Acidic Protein (GFAP) and allograft inflammatory factor-1 (Iba-1) in the sciatic nerve during regeneration.

Sat Sep 23 00:00:00 EDT 2023

Activation of the microglial P2X7R/NLRP3 inflammasome mediates central sensitization in a mouse model of medication overuse headache.

Frontiers in molecular neuroscience

Wang Y,Dong L,Zhang Y,Zhang Y,Qin G,Zhang D,Chen L,He W,Zhou J

MA5-27726 was used in Immunohistochemistry-immunofluorescence to determine how microglial activation and the P2X7R/NLRP3 inflammasome signaling pathway in the trigeminal nucleus caudalis contribute to the pathogenesis of medication overuse headache (MOH).

Sat Jul 01 00:00:00 EDT 2023

Exosomal miR-128-3p reversed fibrinogen-mediated inhibition of oligodendrocyte progenitor cell differentiation and remyelination after cerebral ischemia.

CNS neuroscience & therapeutics

Hou H,Wang Y,Yang L,Wang Y

MA5-27726 was used in Immunohistochemistry-immunofluorescence to investigate the role of exosomal miR-128-3p in promoting fibrinogen-mediated inhibition of oligodendrocyte progenitor cell (OPC) differentiation and the therapeutic potential of exosomal miR-128-3p in cerebral ischemia.

Mon May 01 00:00:00 EDT 2023

Effect of Propionic Acid on Diabetes-Induced Impairment of Unfolded Protein Response Signaling and Astrocyte/Microglia Crosstalk in Rat Ventromedial Nucleus of the Hypothalamus.

Neural plasticity

Natrus LV,Osadchuk YS,Lisakovska OO,Labudzinskyi DO,Klys YG,Chaikovsky YB

MA5-27726 was used in Immunohistochemistry to investigate the influence of propionic acid (PA) on the endoplasmic reticulum (ER), unfolded protein response (UPR) state, and astrocyte/microglia markers in rat ventromedial hypothalamus (VMH) after type 2 diabetes mellitus (T2DM).

Tue Mar 29 00:00:00 EDT 2022

Genipin Attenuates Diabetic Cognitive Impairment by Reducing Lipid Accumulation and Promoting Mitochondrial Fusion via FABP4/Mfn1 Signaling in Microglia.

Antioxidants (Basel, Switzerland)

Liu W,Li K,Zheng M,He L,Chen T

MA5-27726 was used in Immunohistochemistry-immunofluorescence to show that GEN attenuated diabetic cognitive impairment by inhibiting inflammation, lipid accumulation and promoting mitochondrial fusion via FABP4/Mfn1 signaling.

Thu Dec 29 00:00:00 EST 2022

Neuroprotection of cannabidiol in epileptic rats: Gut microbiome and metabolome sequencing.

Frontiers in nutrition

Gong X,Liu L,Li X,Xiong J,Xu J,Mao D,Liu L

MA5-27726 was used in Immunohistochemistry-immunofluorescence to investigate the effects of cannabidiol (CBD) on gut microbiota and the metabolic profile of epileptic rats.

Tue Dec 06 00:00:00 EST 2022

Microglial amyloid beta clearance is driven by PIEZO1 channels.

Journal of neuroinflammation

Jäntti H,Sitnikova V,Ishchenko Y,Shakirzyanova A,Giudice L,Ugidos IF,Gómez-Budia M,Korvenlaita N,Ohtonen S,Belaya I,Fazaludeen F,Mikhailov N,Gotkiewicz M,Ketola K,Lehtonen Š,Koistinaho J,Kanninen KM,Hernández D,Pébay A,Giugno R,Korhonen P,Giniatullin R,Malm T

MA5-27726 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to indicate that the compromised function of microglia in AD could be improved by controlled activation of PIEZO1 channels resulting in alleviated Aβ burden.

Wed Jun 15 00:00:00 EDT 2022

Complement C3a activates astrocytes to promote medulloblastoma progression through TNF-α.

Journal of neuroinflammation

Gong B,Guo D,Zheng C,Ma Z,Zhang J,Qu Y,Li X,Li G,Zhang L,Wang Y

MA5-27726 was used in Immunohistochemistry to reveal the novel role of C3a-mediated TNF-α production by astrocytes in MB progression.

Mon Jun 20 00:00:00 EDT 2022

Sevoflurane Aggravates the Progress of Alzheimer's Disease Through NLRP3/Caspase-1/Gasdermin D Pathway.

Frontiers in cell and developmental biology

Tian D,Xing Y,Gao W,Zhang H,Song Y,Tian Y,Dai Z

MA5-27726 was used in Immunohistochemistry, Immunocytochemistry to study the mechanisms of NLRP3/caspase-1 in pyroptosis and tau pathogenesis induced by sevoflurane.

Tue Feb 08 00:00:00 EST 2022

Sevoflurane Aggravates the Progress of Alzheimer's Disease Through NLRP3/Caspase-1/Gasdermin D Pathway.

Frontiers in cell and developmental biology

Tian D,Xing Y,Gao W,Zhang H,Song Y,Tian Y,Dai Z

MA5-27726 was used in Immunohistochemistry, Immunocytochemistry to study the mechanisms of NLRP3/caspase-1 in pyroptosis and tau pathogenesis induced by sevoflurane.

Tue Feb 08 00:00:00 EST 2022

Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.

Journal of neuroinflammation

Xu X,Gao W,Li L,Hao J,Yang B,Wang T,Li L,Bai X,Li F,Ren H,Zhang M,Zhang L,Wang J,Wang D,Zhang J,Jiao L

Published figure using IBA1 monoclonal antibody (Product # MA5-27726) in Immunocytochemistry

Sat May 22 00:00:00 EDT 2021

Inhibition of Bruton Tyrosine Kinase Reduces Neuroimmune Cascade and Promotes Recovery after Spinal Cord Injury.

International journal of molecular sciences

Yu CG,Bondada V,Iqbal H,Moore KL,Gensel JC,Bondada S,Geddes JW

MA5-27726 was used in Western Blot and Immunohistochemistry to identify BTK as a potential therapeutic target for SCI.

Wed Dec 29 00:00:00 EST 2021

Inhibition of Bruton Tyrosine Kinase Reduces Neuroimmune Cascade and Promotes Recovery after Spinal Cord Injury.

International journal of molecular sciences

Yu CG,Bondada V,Iqbal H,Moore KL,Gensel JC,Bondada S,Geddes JW

MA5-27726 was used in Western Blot and Immunohistochemistry to identify BTK as a potential therapeutic target for SCI.

Wed Dec 29 00:00:00 EST 2021

PD-L1 Improves Motor Function and Alleviates Neuropathic Pain in Male Mice After Spinal Cord Injury by Inhibiting MAPK Pathway.

Frontiers in immunology

Kong F,Sun K,Zhu J,Li F,Lin F,Sun X,Luo X,Ren C,Lu L,Zhao S,Sun J,Wang Y,Shi J

MA5-27726 was used in Immunohistochemistry-immunofluorescence to study the effect and mechanisms of programmed cell death ligand-1 on locomotor and sensory functions and neuropathic pain symptoms following spinal cord injury using a mouse model.

Mon Nov 01 00:00:00 EDT 2021

PD-L1 Improves Motor Function and Alleviates Neuropathic Pain in Male Mice After Spinal Cord Injury by Inhibiting MAPK Pathway.

Frontiers in immunology

Kong F,Sun K,Zhu J,Li F,Lin F,Sun X,Luo X,Ren C,Lu L,Zhao S,Sun J,Wang Y,Shi J

Mon Nov 01 00:00:00 EDT 2021

Sex differences in the expression of the endocannabinoid system within V1M cortex and PAG of Sprague Dawley rats.

Biology of sex differences

Levine A,Liktor-Busa E,Lipinski AA,Couture S,Balasubramanian S,Aicher SA,Langlais PR,Vanderah TW,Largent-Milnes TM

MA5-27726 was used in Immunohistochemistry to suggest that sex differences exist in the endocannabinoid system in two CNS regions relevant to cortical spreading depression and descending modulatory networks in pain/anxiety.

Mon Nov 08 00:00:00 EST 2021

Sex differences in the expression of the endocannabinoid system within V1M cortex and PAG of Sprague Dawley rats.

Biology of sex differences

Levine A,Liktor-Busa E,Lipinski AA,Couture S,Balasubramanian S,Aicher SA,Langlais PR,Vanderah TW,Largent-Milnes TM

Mon Nov 08 00:00:00 EST 2021

NLRP3 inhibition attenuates early brain injury and delayed cerebral vasospasm after subarachnoid hemorrhage.

Journal of neuroinflammation

Dodd WS,Noda I,Martinez M,Hosaka K,Hoh BL

MA5-27726 was used in Immunohistochemistry to observe a decrease in apoptosis of neurons in mice treated with MCC950.

Tue Jul 20 00:00:00 EDT 2021

Microglia-like Cells Promote Neuronal Functions in Cerebral Organoids.

Cells

Fagerlund I,Dougalis A,Shakirzyanova A,Gómez-Budia M,Pelkonen A,Konttinen H,Ohtonen S,Fazaludeen MF,Koskuvi M,Kuusisto J,Hernández D,Pebay A,Koistinaho J,Rauramaa T,Lehtonen Š,Korhonen P,Malm T

MA5-27726 was used in Immunohistochemistry to conclude that microglia-like cells within the organoids promote neuronal and network maturation and recapitulate some aspects of microglia-neuron co-development in vivo, indicating that cerebral organoids could be a useful biorealistic human in vitro platform for studying microglia-neuron interactions.

Thu Dec 30 00:00:00 EST 2021

Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection.

Journal of neuroinflammation

Mahmud FJ,Du Y,Greif E,Boucher T,Dannals RF,Mathews WB,Pomper MG,Sysa-Shah P,Metcalf Pate KA,Lyons C,Carlson B,Chacona M,Brown AM

MA5-27726 was used in Immunohistochemistry to propose that osteopontin may act as a molecular brake within the brain in order to regulate the inflammatory response to chronic viral infections, such as HIV.

Thu Sep 17 00:00:00 EDT 2020

Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection.

Journal of neuroinflammation

Mahmud FJ,Du Y,Greif E,Boucher T,Dannals RF,Mathews WB,Pomper MG,Sysa-Shah P,Metcalf Pate KA,Lyons C,Carlson B,Chacona M,Brown AM

Thu Sep 17 00:00:00 EDT 2020

Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection.

Journal of neuroinflammation

Mahmud FJ,Du Y,Greif E,Boucher T,Dannals RF,Mathews WB,Pomper MG,Sysa-Shah P,Metcalf Pate KA,Lyons C,Carlson B,Chacona M,Brown AM

Thu Sep 17 00:00:00 EDT 2020

SIRT1 Mediates Melatonin's Effects on Microglial Activation in Hypoxia: In Vitro and In Vivo Evidence.

Biomolecules

Merlo S,Luaces JP,Spampinato SF,Toro-Urrego N,Caruso GI,D'Amico F,Capani F,Sortino MA

MA5-27726 was used in Immunohistochemistry-immunofluorescence to provide new evidence for a direct effect of melatonin on hypoxic microglia through SIRT1, which appears as a potential pharmacological target against hypoxic-derived neuronal damage.

Thu Feb 27 00:00:00 EST 2020

SIRT1 Mediates Melatonin's Effects on Microglial Activation in Hypoxia: In Vitro and In Vivo Evidence.

Biomolecules

Merlo S,Luaces JP,Spampinato SF,Toro-Urrego N,Caruso GI,D'Amico F,Capani F,Sortino MA

Thu Feb 27 00:00:00 EST 2020

Oral P. gingivalis impairs gut permeability and mediates immune responses associated with neurodegeneration in LRRK2 R1441G mice.

Journal of neuroinflammation

Feng YK,Wu QL,Peng YW,Liang FY,You HJ,Feng YW,Li G,Li XJ,Liu SH,Li YC,Zhang Y,Pei Z

MA5-27726 was used in Immunohistochemistry-immunofluorescence to investigate the effect of the bacterium porphyromonas gingivalis (Pg) on the gut and brain when administered orally in mice.

Thu Nov 19 00:00:00 EST 2020

Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures.

Cells

Zhang I,Lépine P,Han C,Lacalle-Aurioles M,Chen CX,Haag R,Durcan TM,Maysinger D

MA5-27726 was used in Immunohistochemistry to study glia modulation by dendritic polyglycerol sulfate.

Sat Nov 07 00:00:00 EST 2020

Bioinformatics

Protein Aliases: AIF-1; Allograft inflammatory factor 1; balloon angioplasty responsive transcript; balloon angioplasty-responsive transcript 1; DADB-70P7.8; DASS-82G15.2; Em:AF129756.17; Iba; interferon gamma responsive transcript; ionized calcium binding adapter molecule 1; Ionized calcium-binding adapter molecule 1; Microglia response factor; MRF-1; protein BART-1; Protein G1; testis specific

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Gene Aliases: AI607846; AIF-1; AIF1; BART-1; Bart1; D17H6S50E; G1; IBA1; IRT-1; IRT1; mrf-1; Mrf1

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UniProt ID: (Human) P55008, (Mouse) O70200, (Rat) P55009

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Entrez Gene ID: (Human) 199, (Mouse) 11629, (Rat) 29427

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Function(s)

molecular_function calcium ion binding actin filament binding actin binding protein binding metal ion binding calmodulin-related

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IBA1 Monoclonal Antibody (GT10312)

IBA1 Antibody (MA5-27726) in ICC/IF

Product Details

MA5-27726

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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