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ICAM-1 Monoclonal Antibody (2F9A8)

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Antibody Testing Data (12)

ICAM-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ICAM-1 Antibody (60299-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 60299-1-IG ( ICAM-1 Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ICAM-1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

ICAM-1 Antibody in Flow Cytometry (Flow)

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Product Details

60299-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 30 publications 30 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:200

Immunocytochemistry (ICC/IF)

View 8 publications 8 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2F9A8

Immunogen

ICAM-1 Fusion Protein Ag8309 (181-532 aa encoded by BC015969)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GANFSCRTE LDLRPQGLEL FENTSAPYQL QTFVLPATPP QLVSPRVLEV DTQGTVVCSL DGLFPVSEAQ VHLALGDQRL NPTVTYGNDS FSAKASVSVT AEDEGTQRLT CAVILGNQSQ ETLQTVTIYS FPAPNVILTK PEVSEGTEVT VKCEAHPRAK VTLNGVPAQP LGPRAQLLLK ATPEDNGRSF SCSATLEVAG QLIHKNQTRE LRVLYGPRLD ERDCPGNWTW PENSQQTPMC QAWGNPLPEL KCLKDGTFPL PIGESVTVTR DLEGTYLCRA RSTQGEVTRK VTVNVLSPRY EIVIITVVAA AVIMGTAGLS TYLYNRQRKI KKYRLQQAQK GTPMKPNTQA TPP (181-532 aa encoded by BC015969)

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Ly 47; Major group rhinovirus receptor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells

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Gene Aliases: BB2; CD54; ICAM1; P3.58

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UniProt ID: (Human) P05362

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Entrez Gene ID: (Human) 3383

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Function(s)

virus receptor activity receptor activity transmembrane signaling receptor activity integrin binding protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ICAM-1 Monoclonal Antibody (2F9A8)

ICAM-1 Antibody (60299-1-IG) in IHC (P)

Product Details

60299-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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