Invitrogen

ICAM-1 Recombinant Rabbit Monoclonal Antibody (9H21L19)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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View all (173) ICAM-1 (CD54) antibodies
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Datasheet
Protocols
Questions & Answers

Product Details

701254

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

9H21L19

Immunogen

Peptide corresponding to amino acids 504-517 of human Intercellular adhesion molecule-1
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532442

Product Specific Information

This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Ly 47; Major group rhinovirus receptor; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

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Gene Aliases: BB2; CD54; ICAM; Icam-1; ICAM1; Ly-47; MALA-2; P3.58

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UniProt ID: (Human) P05362, (Mouse) P13597, (Rat) Q00238

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Entrez Gene ID: (Human) 3383, (Mouse) 15894, (Rat) 25464

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Function(s)
virus receptor activity receptor activity transmembrane signaling receptor activity integrin binding protein binding protein complex binding
Process(es)
ovarian follicle development regulation of leukocyte mediated cytotoxicity response to amphetamine T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell acute inflammatory response to antigenic stimulus T cell antigen processing and presentation positive regulation of cellular extravasation cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell aging sensory perception of sound regulation of cell shape response to ionizing radiation response to sulfur dioxide response to organic cyclic compound membrane to membrane docking regulation of cell adhesion extracellular matrix organization positive regulation of actin filament polymerization cellular response to nutrient levels cell adhesion mediated by integrin response to gonadotropin response to drug response to amino acid positive regulation of GTPase activity adhesion of symbiont to host positive regulation of nitric oxide biosynthetic process response to ethanol positive regulation of vasoconstriction response to copper ion viral entry into host cell receptor-mediated virion attachment to host cell positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response leukocyte migration positive regulation of NF-kappaB transcription factor activity negative regulation of calcium ion transport interferon-gamma-mediated signaling pathway establishment of endothelial barrier positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to alkaloid cellular response to glucose stimulus cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to hypoxia establishment of Sertoli cell barrier regulation of ruffle assembly negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of endothelial cell apoptotic process single organismal cell-cell adhesion response to hypoxia response to lipopolysaccharide cellular response to organic substance
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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