Invitrogen

ICAM-2 Monoclonal Antibody (3C4), Alexa Fluor™ 647

ICAM-2 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

ICAM-2 Antibody (A15452) in Flow

Flow cytometry analysis of ICAM-2 in BALB/c mouse splenocytes. Cells were stained using ICAM-2 Monoclonal Antibody (3C4), Alexa Fluor™ 647 (Product # A15452).

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Product Details

A15452

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 6 publications 6 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

3C4

Immunogen

Mouse CD102 from COS cells transfected with mouse ICAM-2 cDNA and BW5147 AKR/J mouse thymoma cell line

View immunogen

Conjugate

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

Excitation/Emission Max

650/671 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

<0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2534465

Target Information

ICAM-2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM-2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. Diseases associated with ICAM2 include Colon Carcinoma In Situ.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Three-axis classification of mouse lung mesenchymal cells reveals two populations of myofibroblasts.

Development (Cambridge, England)

Narvaez Del Pilar O,Gacha Garay MJ,Chen J

A15452 was used in Flow cytometry/Cell sorting to classify the mouse lung mesenchyme into three proximal-distal axes that are associated with the endothelium, epithelium and interstitium, respectively.

Tue Mar 15 00:00:00 EDT 2022

Differential chromatin binding of the lung lineage transcription factor NKX2-1 resolves opposing murine alveolar cell fates in vivo.

Nature communications

Little DR,Lynch AM,Yan Y,Akiyama H,Kimura S,Chen J

A15452 was used in Flow cytometry/Cell sorting to report that the cell-type-specific function of NKX2-1 is attributed to its differential chromatin binding that is acquired or retained during development in coordination with partner transcriptional factors.

Tue May 04 00:00:00 EDT 2021

Single-Cell RNA Sequencing Reveals Renal Endothelium Heterogeneity and Metabolic Adaptation to Water Deprivation.

Journal of the American Society of Nephrology : JASN

Dumas SJ,Meta E,Borri M,Goveia J,Rohlenova K,Conchinha NV,Falkenberg K,Teuwen LA,de Rooij L,Kalucka J,Chen R,Khan S,Taverna F,Lu W,Parys M,De Legher C,Vinckier S,Karakach TK,Schoonjans L,Lin L,Bolund L,Dewerchin M,Eelen G,Rabelink TJ,Li X,Luo Y,Carmeliet P

A15452 was used in Flow cytometry/Cell sorting to provide a high-resolution atlas of the renal endothelium and highlights extensive renal endothelial cell phenotypic heterogeneity, as well as a previously unrecognized role of oxidative phosphorylation in the metabolic adaptation of medullary renal endothelial cells to water deprivation.

Wed Jan 01 00:00:00 EST 2020

Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung.

Developmental cell

Vila Ellis L,Cain MP,Hutchison V,Flodby P,Crandall ED,Borok Z,Zhou B,Ostrin EJ,Wythe JD,Chen J

A15452 was used in Flow cytometry/Cell sorting to show that VEGFA from the epithelium is required for a distinct endothelial cell (EC) population in the mouse lung.

Mon Mar 09 00:00:00 EDT 2020

Redundant and additive functions of the four Lef/Tcf transcription factors in lung epithelial progenitors.

Proceedings of the National Academy of Sciences of the United States of America

Gerner-Mauro KN,Akiyama H,Chen J

A15452 was used in Flow cytometry/Cell sorting to generate two quadruple conditional knockouts, four triple mutants, and various combinations of double mutants, showing that the four Lef/Tcf genes function redundantly in the presence of at least two Lef/Tcf paralogs, but additively upon losing additional paralogs to specify and maintain lung epithelial progenitors., using the developing mouse lung as a model system.

Tue Jun 02 00:00:00 EDT 2020

Quantitative single-cell interactomes in normal and virus-infected mouse lungs.

Disease models & mechanisms

Cain MP,Hernandez BJ,Chen J

A15452 was used in Flow cytometry/Cell sorting to describe a quantitative intuitive algorithm, coupled with an optimized experimental protocol, to construct and compare interactomes as a Control and Sendai virus-infected mouse lungs.

Fri Jun 26 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: CD102; ICAM-2; Intercellular adhesion molecule 2; Lymphocyte function-associated AG-1 counter-receptor; RP23-418O11.10-001

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Gene Aliases: CD102; Icam-2; Icam2; Ly-60

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UniProt ID: (Mouse) P35330

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Entrez Gene ID: (Mouse) 15896

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Function(s)

integrin binding protein binding

Process(es)

cell adhesion single organismal cell-cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ICAM-2 Monoclonal Antibody (3C4), Alexa Fluor™ 647

ICAM-2 Antibody (A15452) in Flow

Product Details

A15452

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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