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ID4 Antibody (CF804977) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID4 (RC204170, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ID4. Positive lysates LY419876 (100 µg) and LC419876 (20 µg) can be purchased separately from OriGene.
Product Details
CF804977
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2G10
Immunogen
Full length human recombinant protein of human ID4 produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
ID4 is transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHb27; Class B basic helix-loop-helix protein 27; DNA-binding protein inhibitor ID-4; Inhibitor of differentiation 4; Inhibitor of DNA binding 4; inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
Gene Aliases: BHLHB27; ID4; IDB4
UniProt ID: (Human) P47928
Entrez Gene ID: (Human) 3400
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
neuroblast proliferation
circadian rhythm
positive regulation of cell proliferation
hippocampus development
cerebral cortex neuron differentiation
central nervous system myelination
cellular protein localization
fat cell differentiation
negative regulation of fat cell differentiation
negative regulation of neuron differentiation
positive regulation of osteoblast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of astrocyte differentiation
negative regulation of oligodendrocyte differentiation
prostate gland epithelium morphogenesis
prostate gland stromal morphogenesis
seminal vesicle morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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