Invitrogen

IDO Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (97) IDO antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

IDO Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

IDO Antibody (PA5-24598) in IHC (P)

Immunohistochemistry analysis of IDO in formalin fixed and paraffin embedded human lung carcinoma. Samples were incubated with IDO polyclonal antibody (Product # PA5-24598) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

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IDO Antibody — Figure 4 The effects of PG2 on the expression and function of IDO. PG2 inhibited IDO protein expression in (A) B16F10 and (B) LL2 cells in a dose-dependent manner. After treatment with PG2 (0-100 ng/ml) for 24 h, the expression of IDO and TDO levels in B16F10 and LL2 cells were measured by Western blotting. The kynurenine assay was used for the kynurenine production in (C) B16F10 and (D) LL2 cells. The conditioned medium of (E) B16F10 and (F) LL2 after treated with indicated concentrations of PG2 for 24 h mixed with an equal amount of original medium. T cells were cultured in media conditioned from tumor cells for 72 h. The cell number were measured by staining with trypan blue. (n = 6, data are mean+- SD. * P < 0.05). PG2 induced Cx43 and inhibited IDO protein expression in (G) A549 and (H) PC9 cells in a dose-dependent manner. After treatment with PG2 (0-100 ng/ml) for 24 h, the expression of Cx43 and IDO levels in A549 and PC9 cells were measured by Western blotting. {TM}

Product Details

PA5-24598

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 79-105 amino acids from the central region of human INDO

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Ammonium sulfate precipitation, Size-exclusion - Dialysis

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2542098

Target Information

IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin. By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction. The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The extracts of Astragalus membranaceus enhance chemosensitivity and reduce tumor indoleamine 2, 3-dioxygenase expression.

International journal of medical sciences

Phacharapiyangkul N,Wu LH,Lee WY,Kuo YH,Wu YJ,Liou HP,Tsai YE,Lee CH

Published figure using IDO polyclonal antibody (Product # PA5-24598) in Western Blot

Fri Jan 31 00:00:00 EST 2020

Bioinformatics

Protein Aliases: EC 1.13.11.52; IDO-1; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine; Indoleamine 2,3-dioxygenase 1; Indoleamine-2; indoleamine-pyrrole 2,3 dioxygenase; Indoleamine-pyrrole 2,3-dioxygenase

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Gene Aliases: IDO; IDO-1; IDO1; INDO

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UniProt ID: (Human) P14902, (Mouse) P28776

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Entrez Gene ID: (Human) 3620, (Mouse) 15930

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Function(s)

tryptophan 2,3-dioxygenase activity electron carrier activity heme binding indoleamine 2,3-dioxygenase activity metal ion binding protein binding oxidoreductase activity amino acid binding oxygen binding dioxygenase activity oxygenase

Process(es)

immune system process cytokine production involved in inflammatory response positive regulation of T cell tolerance induction positive regulation of chronic inflammatory response positive regulation of type 2 immune response tryptophan catabolic process female pregnancy tryptophan catabolic process to kynurenine response to lipopolysaccharide negative regulation of interleukin-10 production positive regulation of interleukin-12 production multicellular organismal response to stress kynurenic acid biosynthetic process swimming behavior negative regulation of T cell proliferation regulation of activated T cell proliferation oxidation-reduction process negative regulation of T cell apoptotic process positive regulation of T cell apoptotic process defense response inflammatory response positive regulation of apoptotic process negative regulation of activated T cell proliferation

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IDO Polyclonal Antibody

IDO Antibody (PA5-24598) in IHC (P)

Product Details

PA5-24598

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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