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Invitrogen
IDO Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
IDO Antibody (PA5-85393) in WB
Knockout of IDO1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR864924_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of IDO1 was performed by loading 30 µg of HeLa wild type (Lane 1), HeLa wild type treated with 50 ng/mL IFNgamma for 24hrs (Lane 2), HeLa Cas9 (Lane 3), HeLa Cas9 treated with 50 ng/mL IFNgamma for 24hrs (Lane 4), HeLa IDO1 KO (Lane 5) and HeLa IDO1 KO treated with 50 ng/mL IFNgamma for 24hrs (Lane 6) whole cell extracts. The samp... View More
Product Details
PA5-85393
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human IDO1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792535
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Rhesus Monkey (97%).
Positive Control: A549 (starvation for 24 hr, and 100 ng/mL IFN-gamma treatment for 48 hr), IDO1-transfected 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin. By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction. The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 1.13.11.52; IDO-1; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine; Indoleamine 2,3-dioxygenase 1; Indoleamine-2; Indoleamine-pyrrole 2,3-dioxygenase
Gene Aliases: IDO; IDO-1; IDO1; INDO
UniProt ID: (Human) P14902
Entrez Gene ID: (Human) 3620
immune system process
cytokine production involved in inflammatory response
positive regulation of T cell tolerance induction
positive regulation of chronic inflammatory response
positive regulation of type 2 immune response
tryptophan catabolic process
female pregnancy
tryptophan catabolic process to kynurenine
response to lipopolysaccharide
negative regulation of interleukin-10 production
positive regulation of interleukin-12 production
multicellular organismal response to stress
kynurenic acid biosynthetic process
swimming behavior
negative regulation of T cell proliferation
regulation of activated T cell proliferation
oxidation-reduction process
negative regulation of T cell apoptotic process
positive regulation of T cell apoptotic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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