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Invitrogen
IDO Monoclonal Antibody (eyedio), eFluor™ 450, eBioscience™
FIGURE: 1 / 4
IDO Antibody (48-9477-42) in Flow
Normal human peripheral blood cells were stimulated overnight with LPS (Product # 00-4976-03), surface stained with CD11c Monoclonal Antibody, APC (Product # 17-0116-42). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with Mouse IgG1 kappa Isotype Control, eFluor 450 (Product # 48-4714-82) (left) or IDO Monoclonal Antibody, eFluor 450 (right). Cells in the monocyte gate were used for analysis.
Product Details
48-9477-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eyedio
Conjugate
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2784731
Product Specific Information
Description: This eyedio monoclonal antibody reacts with human indoleamine-2,3-dioxygenase (IDO, INDO, IDO1), an intracellular enzyme that catalyzes the degradation of tryptophan to kynurenines. IDO is expressed in a wide variety of tissues and cells, including macrophages, plasmacytoid dendritic cells, and several cell lines. IDO can be induced in many different cell types by IFN gamma or other inflammatory stimuli. Expression of IDO in antigen presenting cells and tumors is thought to mediate immune suppression through depletion of this essential amino acid and/or by the creation of tryptophan metabolites that cause apoptosis of T cells and induction of regulatory T cells.
Applications Reported: This eyedio antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eyedio antibody has been pre-diluted and tested by flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located on the Resources Tab online. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor 450 is an alternative to Pacific Blue. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Target Information
IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin. By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction. The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: EC 1.13.11.52; IDO-1; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine; Indoleamine 2,3-dioxygenase 1; Indoleamine-2; Indoleamine-pyrrole 2,3-dioxygenase
Gene Aliases: IDO; IDO-1; IDO1; INDO
UniProt ID: (Human) P14902
Entrez Gene ID: (Human) 3620
immune system process
cytokine production involved in inflammatory response
positive regulation of T cell tolerance induction
positive regulation of chronic inflammatory response
positive regulation of type 2 immune response
tryptophan catabolic process
female pregnancy
tryptophan catabolic process to kynurenine
response to lipopolysaccharide
negative regulation of interleukin-10 production
positive regulation of interleukin-12 production
multicellular organismal response to stress
kynurenic acid biosynthetic process
swimming behavior
negative regulation of T cell proliferation
regulation of activated T cell proliferation
oxidation-reduction process
negative regulation of T cell apoptotic process
positive regulation of T cell apoptotic process
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