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Invitrogen
IDO Chimeric Recombinant Mouse Monoclonal Antibody (7H8L17)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
IDO Chimeric Antibody (702743M) in ICC/IF
Immunofluorescent analysis of IDO1 was performed using 70% confluent A549 cells with and without treatment of human IFNγ (50 ng/mL for 24 hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with IDO1 Chimeric Recombinant Mouse Monoclonal Antibody (7H8L17) (Produ... View More
Product Details
702743M
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG
Expression System
ExpiCHO
Class
Recombinant Monoclonal
Type
Antibody
Clone
7H8L17
Immunogen
Protein corresponding to human IDO (aa1-aa403)
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
This antibody has Rabbit Variable heavy and light chains, and Mouse constant domains. It recognizes human IDO protein. This chimeric antibody is recognized by anti-mouse secondary antibodies and has no cross reactivity to anti-rabbit secondary antibodies.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin. By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction. The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 1.13.11.52; IDO-1; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine; Indoleamine 2,3-dioxygenase 1; Indoleamine-2; Indoleamine-pyrrole 2,3-dioxygenase
Gene Aliases: IDO; IDO-1; IDO1; INDO
UniProt ID: (Human) P14902
Entrez Gene ID: (Human) 3620
immune system process
cytokine production involved in inflammatory response
positive regulation of T cell tolerance induction
positive regulation of chronic inflammatory response
positive regulation of type 2 immune response
tryptophan catabolic process
female pregnancy
tryptophan catabolic process to kynurenine
response to lipopolysaccharide
negative regulation of interleukin-10 production
positive regulation of interleukin-12 production
multicellular organismal response to stress
kynurenic acid biosynthetic process
swimming behavior
negative regulation of T cell proliferation
regulation of activated T cell proliferation
oxidation-reduction process
negative regulation of T cell apoptotic process
positive regulation of T cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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