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Invitrogen
IFITM1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
IFITM1 Antibody (PA5-27495) in ICC/IF
Immunofluorescence analysis of Interferon-induced transmembrane protein 1 was performed using 70% confluent log phase MCF7 treated with Human IFN Alpha (1000u/mL for 12hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IFITM1 Polyclonal Antibody (Product # PA5-27495) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H... View More
Product Details
PA5-27495
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 101 of Human IFITM1
Conjugate
Form
Liquid
Concentration
0.14 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2544971
Product Specific Information
Recommended positive controls: A549, HeLa.
Predicted reactivity: Chimpanzee (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
IFITM1 (Interferon inducible transmembrane protein 1) is a member of the IFN-inducible transmembrane protein family. It is an essential mediator of interferon-gamma-induced antiproliferation and plays a role in the control of cell growth. IFITM1 is upregulated in several tumor types and may be useful as a tumor biomarker. Both mouse IFITM1 and IFITM3 are expressed on the cell surfaces of primordial germ cells in a developmentally-regulated manner. IFITM1 activity is required for primordial germ cell transit, and IFITM1 acts as a repulsive molecule by repelling non-IFITM1-expressing primordial germ cells from the mesoderm into the endoderm.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 27-Sep; CD225; Dispanin subfamily A member 2a; DSPA2a; Fragilis protein 2; fragilis2; ifitm-like protein 2; interferon induced transmembrane protein 2 like; Interferon-induced protein 17; Interferon-induced transmembrane protein 1; Interferon-inducible protein 9-27; Leu-13 antigen; Mil-2; Mouse ifitm-like protein 2
Gene Aliases: 1110036C17Rik; 9-27; CD225; DSPA2a; IFI17; IFITM1; LEU13; Mil-2; Mil2
UniProt ID: (Human) P13164, (Mouse) Q9D103
Entrez Gene ID: (Human) 8519, (Mouse) 68713
ossification
cell surface receptor signaling pathway
negative regulation of cell proliferation
response to virus
negative regulation of cell migration
response to interferon-gamma
response to interferon-alpha
response to interferon-beta
negative regulation of viral genome replication
positive regulation of osteoblast differentiation
negative regulation of viral entry into host cell
regulation of immune response
defense response to virus
type I interferon signaling pathway
somitogenesis
immune system process
response to biotic stimulus
anterior/posterior pattern specification
innate immune response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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