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Invitrogen
IFITM3 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
IFITM3 Antibody (PA5-30382) in ICC/IF
Immunofluorescence analysis of IFITM3 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IFITM3 Polyclonal Antibody (Product # PA5-30382) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2000 ... View More
Product Details
PA5-30382
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length human IFITM3 Recombinant protein.
Conjugate
Form
Liquid
Concentration
0.74 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547856
Product Specific Information
Recommended positive controls: HeLa.
Predicted reactivity: Chimpanzee (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
IFITM3 (interferon induced transmembrane protein 3), also known as 1-8U or IP15, is a multi-pass membrane protein that belongs to the IFITM (interferon inducible transmembrane) family of proteins. IFITM proteins are induced by type I and type II interferons and contain multiple interferon (IFN)-stimulated response elements (ISREs) in their promoter regions. IFITM proteins play important roles in many cellular processes and their expression requires the presence of the chromatin remodeling SWI/SNF-like BAF complexes. Cellular processes involving IFITM proteins include cellular anti-proliferative activities and homotypic cell adhesion functions of interferons. In addition, IFITM genes are often upregulated in various cancer cells, suggesting a possible role in carcinogenesis. Localizing to the membrane, IFITM3 is a 133 amino acid protein that is induced by IFN-alpha and IFN-gamma. IFITM3 expression can be regulated by TEF-1, Brg-1 and Sp1.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Dispanin subfamily A member 2b; DSPA2b; Fragilis; Fragilis 3; Fragilis protein; ifitm-like protein 1; Interferon-induced transmembrane protein 3; Interferon-inducible protein 1-8U; Interferon-inducible protein 15; Mil-1; Mouse ifitm-like protein 1
Gene Aliases: 1-8U; 1110004C05Rik; Cd225; Cdw217; DSPA2b; Fgls; IFITM3; IP15; mil-1
UniProt ID: (Human) Q01628, (Mouse) Q9CQW9
Entrez Gene ID: (Human) 10410, (Mouse) 66141
immune system process
receptor-mediated endocytosis
negative regulation of cell proliferation
response to biotic stimulus
response to virus
negative regulation of viral transcription
response to interferon-gamma
response to interferon-alpha
response to interferon-beta
negative regulation of viral genome replication
innate immune response
negative regulation of viral entry into host cell
defense response to virus
type I interferon signaling pathway
immune response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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