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Invitrogen
IFN alpha Monoclonal Antibody (EBI-1), eBioscience™
Product Details
BMS160
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
EBI-1
Immunogen
Rec. Human IFN alpha
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Ion-exchange chromatography, Ammonium sulfate precipitation
Storage buffer
PBS
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice
RRID
AB_10597749
Product Specific Information
Description: Anti-Human IFN-alpha (EBI-1) inhibits the antiviral activity of interferon alpha. BMS160 specifically reacts with human recombinant and natural interferon alpha and can be used as capture antibody in ELISA.
Applications Tested: ELISA, Functional Studies (Blocking).
Source: E. coli (amino acids Ser113-Ala271; accession # NP_000566).
Bioactivity: The ED50 of this protein, as measured by proliferation of mouse D10S cells, is less than or equal to 0.001 ng/mL, which corresponds to a specific activity of at least 1.0 x 10e9 Units/mg.
Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC.
Molecular Weight: 18 kDa.
Storage and handling: For best recovery, quick spin vial prior to opening. Use in sterile environment. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Target Information
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha-2a interferon; ifa; IFN alpha 1/13; IFN alpha 1b; IFN α; IFN-alpha 1b; IFN-alpha-1/13; IFN-alpha-2; IFNAalphaIFN-alpha-1/13; IFNalpha; IFNα; Interferon alpha 13 (IFNA13); interferon alpha 1b; interferon alpha 2a; interferon alpha 2b; interferon alpha A; Interferon alpha leukocyte (IFL); Interferon alpha-1/13; Interferon alpha-1/13 (IFN-alpha-1/13); Interferon alpha-2; Interferon alpha-A; Interferon alpha-D; Interferon alpha-D (IFN alphaD); Interferon alpha1 (IFNA1); interferon, alpha 1; interferon, alpha 2; interferon-alpha1; LeIF A; LeIF D; MGC138207; MGC138505; MGC138507
Gene Aliases: IFL; IFN; IFN-ALPHA; IFN-alphaA; IFN-alphaD; IFNA; IFNA1; IFNA13; IFNA2; IFNA2A; IFNA2B; IFNA2C; IFNA@; INFA2
UniProt ID: (Human) P01562, (Human) P01563
Entrez Gene ID: (Human) 3439, (Human) 3440
adaptive immune response
T cell activation involved in immune response
natural killer cell activation involved in immune response
humoral immune response
blood coagulation
cytokine-mediated signaling pathway
B cell differentiation
positive regulation of peptidyl-serine phosphorylation of STAT protein
B cell proliferation
response to exogenous dsRNA
innate immune response
defense response to virus
type I interferon signaling pathway
regulation of type I interferon-mediated signaling pathway
apoptotic process
inflammatory response
cell surface receptor signaling pathway
cell-cell signaling
negative regulation of gene expression
positive regulation of phosphorylation
positive regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of T cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
negative regulation of viral entry into host cell
negative regulation of T-helper 2 cell cytokine production
negative regulation of interleukin-5 secretion
negative regulation of interleukin-13 secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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