Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
IFN alpha Monoclonal Antibody (RMMA-1)
FIGURE: 1 / 1
IFN alpha Antibody (221001) in ELISA
Communications biology 2020 -
Fig. 2 Increased levels of neutrophil regulating factors in retinas from Cryba1 cKO mice and human early AMD donor eyes. The levels of a CXCL1, b IFNalpha, and c IFNlamda were increased in the RPE-choroid tissue homogenate of 15-month-old Cryba1 cKO mice compared to age-matched Cryba1 fl/fl controls as measured by ELISA. No changes were observed in 3-month-old mice. n = 6. * P < 0.05 (one-way ANOVA and Tuke... View More
Product Details
221001
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Rat
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
RMMA-1
Immunogen
mouse interferon alpha
Conjugate
Form
Liquid
Concentration
2.5 mg/mL
Purification
Affinity chromatography, Ion-exchange chromatography, Size-exclusion chromatography
Storage buffer
0.3M sodium bicarbonate, pH 8.0, with 0.2M NaCl
Contains
no preservative
Storage conditions
-80° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_223544
Product Specific Information
221001 targets IFN alpha in ELISA and Neutralization applications and shows reactivity with mouse samples. Purity is greater than 95%, and endotoxin level is less than 1 EU/ug.
In neutralization assays, one neutralization unit is the amount of antiserum required to neutralize one unit of mouse IFN alpha to a 50% endpoint. This antibody is expected to work in IP applications, although it has not been fully evaluated for IP.
After receipt, the product may be stored at -20C for short-term use (under 6 months). For long-term storage, the product should be kept at -70C or below for retention of full activity.
IFN alpha has a predicted molecular weight of approximately 19 kDa.
Target Information
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ifa; IFN alpha 1/13; IFN alpha 1b; IFN α; IFN-alpha-1; IFN-alpha-2; IFNAalphaIFN-alpha-1/13; IFNalpha; IFNα; Interferon alpha 13 (IFNA13); interferon alpha 15; interferon alpha family gene 1; interferon alpha family, gene 2; Interferon alpha leukocyte (IFL); Interferon alpha-1; Interferon alpha-1/13 (IFN-alpha-1/13); interferon alpha-2; Interferon alpha-D (IFN alphaD); Interferon alpha1 (IFNA1); MGC138207; MGC138505; MGC138507; novel interferon alpha (Ifna) family member
Gene Aliases: Gm12597; Ifa1; Ifa2; Ifna1; Ifnaa; MuIFN-alpha-A; OTTMUSG00000007655
UniProt ID: (Mouse) P01572
Entrez Gene ID: (Mouse) 15962, (Mouse) 242517, (Mouse) 15965
adaptive immune response
T cell activation involved in immune response
natural killer cell activation involved in immune response
defense response
humoral immune response
cytokine-mediated signaling pathway
B cell differentiation
positive regulation of peptidyl-serine phosphorylation of STAT protein
B cell proliferation
defense response to bacterium
response to exogenous dsRNA
defense response to virus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support