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Leinco Technologies
IFN beta Monoclonal Antibody (HDb-4A7), Functional Grade, In Vivo GOLD™
Product Details
I-1182-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
HDb-4A7
Immunogen
Plasmid DNA encoding murine IFNb
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.2-7.4
Contains
no preservative
Storage conditions
4°C short term, -80°C long term
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Specificity: Mouse Anti-Mouse Interferon-beta (IFN-Beta) (Clone HDBeta-4A7) recognizes an epitope on Mouse IFN-Beta and binds specifically to murine IFN-Beta and does not bind murine IFN-gamma or several different IFN-alpha species (a-1,4,5 or 13); HDb-4A7 binds both recombinant and natural forms. This antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Endotoxin Level: ≤ 1.0 EU/mg as determined by the LAL method. Purity: >95% by SDS Page; ≥95% monomer by analytical SEC.
Product Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8 C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80 C. Avoid Repeated Freeze Thaw Cycles.
This antibody has been tested in the following applications: Western Blotting, Neutralization, Functional Assay, Blocking.
Target Information
Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: IFN b; IFN β; IFN-beta; IFNbeta; IFNβ; Interferon b; Interferon beta; RP11-113D19.1
Gene Aliases: Ifb; IFN-beta; IFNB; Ifnb1
UniProt ID: (Mouse) P01575
Entrez Gene ID: (Mouse) 15977
adaptive immune response
T cell activation involved in immune response
B cell activation involved in immune response
natural killer cell activation involved in immune response
defense response
humoral immune response
cytokine-mediated signaling pathway
B cell differentiation
positive regulation of peptidyl-serine phosphorylation of STAT protein
cellular response to interferon-beta
B cell proliferation
defense response to bacterium
response to exogenous dsRNA
negative regulation of viral genome replication
negative regulation of T cell differentiation
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
type I interferon signaling pathway
cellular response to dexamethasone stimulus
negative regulation of T-helper 2 cell cytokine production
positive regulation of apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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