Invitrogen

IFN gamma Polyclonal Antibody, PeproTech®

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IFN gamma Antibody (500-P119-1MG) in WB

Western Blot: To detect Murine IFN-gamma by Western Blot analysis IFN gamma Polyclonal Antibody (Product # 500-P119-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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Product Details

500-P119-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 5 publications 5 publications

Neutralization (Neu)

View 2 publications 2 publications

Blocking Assay (BLOCK)

View 1 publication 1 publication

In vitro Assay (IV)

View 11 publications 11 publications

Immunostaining (IS)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine IFN-gamma

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929307

Product Specific Information

AA Sequence of recombinant protein: MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IFN-gamma. Anti-Murine IFN-gamma-specific antibody was purified by affinity chromatography employing an immobilized Murine IFN-gamma matrix.

Sandwich ELISA: To detect Murine IFN-gamma by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IFN-gamma (500-P119Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IFN-gamma.

Western Blot: To detect Murine IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Enterocytes, fibroblasts and myeloid cells synergize in anti-bacterial and anti-viral pathways with IL22 as the central cytokine.

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Delacher M,Imbusch CD,Hotz-Wagenblatt A,Mallm JP,Bauer K,Simon M,Riegel D,Rendeiro AF,Bittner S,Sanderink L,Pant A,Schmidleithner L,Braband KL,Echtenachter B,Fischer A,Giunchiglia V,Hoffmann P,Edinger M,Bock C,Rehli M,Brors B,Schmidl C,Feuerer M

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Mondragón L,Mhaidly R,De Donatis GM,Tosolini M,Dao P,Martin AR,Pons C,Chiche J,Jacquin M,Imbert V,Proïcs E,Boyer L,Doye A,Luciano F,Neels JG,Coutant F,Fabien N,Sormani L,Rubio-Patiño C,Bossowski JP,Muller F,Marchetti S,Villa E,Peyron JF,Gaulard P,Lemonnier F,Asnafi V,Genestier L,Benhida R,Fournié JJ,Passeron T,Ricci JE,Verhoeyen E

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Th2-associated alternative Kupffer cell activation promotes liver fibrosis without inducing local inflammation.

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Bioinformatics

Protein Aliases: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

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Gene Aliases: Ifg; IFN-g; Ifng

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UniProt ID: (Mouse) P01580

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Entrez Gene ID: (Mouse) 15978

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Function(s)

cytokine activity interferon-gamma receptor binding protein binding cytokine interferon superfamily intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IFN gamma Polyclonal Antibody, PeproTech®

IFN gamma Antibody (500-P119-1MG) in WB

Product Details

500-P119-1MG

Western Blot (WB)

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Blocking Assay (BLOCK)

In vitro Assay (IV)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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