Invitrogen

IFN gamma Polyclonal Antibody

Advanced Verification

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.

View all (214) IFN gamma antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-47943

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1 µg/mL
-

Immunocytochemistry (ICC/IF)

5-15 µg/mL
-

Neutralization (Neu)

<8 µg/mL
-
Product Specifications

Species Reactivity

Dog

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant canine IFN- gamma Gln24-Lys166
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2608799

Product Specific Information

In direct ELISAs, less than 40% cross-reactivity with recombinant porcine IFN- gamma is observed, approximately 20% cross-reactivity with recombinant feline IFN- gamma is observed, and less than 5% cross-reactivity with recombinant human IFN-gamma, recombinant rhesus macaque IFN-gamma, recombinant mouse IFN- gamma, and recombinant rat IFN- gamma is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cytokine; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

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Gene Aliases: IFN-G; IFN-gamma; IFNG

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UniProt ID: (Dog) P42161

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Entrez Gene ID: (Dog) 403801

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Function(s)
interferon-gamma receptor binding cytokine interferon superfamily intercellular signal molecule
Process(es)
microglial cell activation macrophage activation involved in immune response cell cycle arrest positive regulation of autophagy positive regulation of epithelial cell migration regulation of protein ADP-ribosylation macrophage differentiation positive regulation of protein complex assembly negative regulation of interleukin-17 production positive regulation of interleukin-12 production positive regulation of interleukin-23 production positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response positive regulation of peptidyl-serine phosphorylation of STAT protein positive regulation of smooth muscle cell apoptotic process regulation of growth positive regulation of protein import into nucleus positive regulation of tyrosine phosphorylation of STAT protein positive regulation of chemokine biosynthetic process positive regulation of nitric oxide biosynthetic process positive regulation of osteoclast differentiation negative regulation of transcription, DNA-templated astrocyte activation negative regulation of smooth muscle cell proliferation positive regulation of cytokine secretion positive regulation of inflammatory response positive regulation of neurogenesis regulation of insulin secretion positive regulation of membrane protein ectodomain proteolysis defense response to virus positive regulation of killing of cells of other organism interferon-gamma-mediated signaling pathway positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity positive regulation of fructose 1,6-bisphosphate metabolic process positive regulation of calcidiol 1-monooxygenase activity positive regulation of protein serine/threonine kinase activity positive regulation of protein deacetylation extrinsic apoptotic signaling pathway positive regulation of neuron death positive regulation of beta-amyloid formation negative regulation of tau-protein kinase activity positive regulation of core promoter binding positive regulation of tumor necrosis factor (ligand) superfamily member 11 production
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