Invitrogen

IFN gamma Monoclonal Antibody (67F12A8), Biotin

FIGURE: 1 / 5

IFN gamma Antibody (AHC4539) in ELISA

Nature communications 2017 - Fig. 1 Binding of human glycosylated cytokines to galectin-3-coated beads. Ratio refers to molar ratio. a hIFNgamma measured by ELISA in the supernatant after incubation with galectin-3-coated beads in the presence or absence of 100 mM lactose. The glycosylated IFNgamma was produced in CHO cells and the unglycosylated IFNgamma was produced in E. coli . Mean +- SD of one representative experiment of 8 (glyco... View More

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Product Details

AHC4539

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

View 13 publications 13 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

67F12A8

Immunogen

Recombinant human interferon-gamma.

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 5mg/mL BSA

Contains

0.5mg/mL sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536281

Product Specific Information

This product is recommended as a detection antibody in Sandwich ELISA applications.

This clone can also be referred to as clone number 67F 12A8 L1.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Galectin-3 captures interferon-gamma in the tumor matrix reducing chemokine gradient production and T-cell tumor infiltration.

Nature communications

Gordon-Alonso M,Hirsch T,Wildmann C,van der Bruggen P

Published figure using IFN gamma monoclonal antibody (Product # AHC4539) in ELISA

Fri Oct 06 00:00:00 EDT 2017

A Homogeneous Immunoassay Method for Detecting Interferon-Gamma in Patients with Latent Tuberculosis Infection.

Journal of microbiology and biotechnology

Wu F,Wang L,Guo Q,Zhao M,Gu H,Xu H,Lou J

AHC4539 was used to utilize a homogeneous immunoassay method to identify interferon-gamma in patients with latent tuberculosis infection

Tue Mar 01 00:00:00 EST 2016

Genetic immunisation by liver stage antigen 3 protects chimpanzees against malaria despite low immune responses.

PloS one

Daubersies P,Ollomo B,Sauzet JP,Brahimi K,Perlaza BL,Eling W,Moukana H,Rouquet P,de Taisne C,Druilhe P

AHC4539 was used in ELISA to discuss the hurdles involved in human genetic immunization

Wed Jul 16 00:00:00 EDT 2008

Interleukin-12 is involved in the enhancement of human natural killer cell activity by Lactobacillus casei Shirota.

Clinical and experimental immunology

Takeda K,Suzuki T,Shimada SI,Shida K,Nanno M,Okumura K

AHC4539 was used in ELISA to evaluate the effect of Lactobacillus casei Shirota on human natural killer cell activity

Sun Oct 01 00:00:00 EDT 2006

Distinct indirect pathways govern human NK-cell activation by TLR-7 and TLR-8 agonists.

International immunology

Gorski KS,Waller EL,Bjornton-Severson J,Hanten JA,Riter CL,Kieper WC,Gorden KB,Miller JS,Vasilakos JP,Tomai MA,Alkan SS

AHC4539 was used in ELISA to analyze the governing of human NK-cell activation by TLR-7 and TLR-8 agonists by distinct indirect pathways

Sat Jul 01 00:00:00 EDT 2006

The effect of aging and inflammation on heat shock protein 27 in human monocytes and lymphocytes.

Experimental gerontology

Njemini R,Lambert M,Demanet C,Mets T

AHC4539 was used in ELISA to examine the effect of age and inflammation on the induction of Hsp27 in human peripheral blood mononuclear cells

Wed Mar 01 00:00:00 EST 2006

Impaired interleukin (IL)-4-associated generation of CCR4-expressing T cells in neonates with hereditary allergy risk.

Clinical and experimental immunology

Haddeland U,Sletten GB,Brandtzaeg P,Nakstad B

AHC4539 was used in ELISA to investigate how early antigen encounters may influence allergy

Tue Feb 01 00:00:00 EST 2005

Methylation-dependent T cell immunity to Mycobacterium tuberculosis heparin-binding hemagglutinin.

Nature medicine

Temmerman S,Pethe K,Parra M,Alonso S,Rouanet C,Pickett T,Drowart A,Debrie AS,Delogu G,Menozzi FD,Sergheraert C,Brennan MJ,Mascart F,Locht C

AHC4539 was used in ELISA to report that methylation of the Mycobacterium tuberculosis heparin-binding hemagglutinin is essential for effective T cell immunity to this antigen

Wed Sep 01 00:00:00 EDT 2004

Oxidative stress as a trigger for cellular immune responses in patients with alcoholic liver disease.

Hepatology (Baltimore, Md.)

Stewart SF,Vidali M,Day CP,Albano E,Jones DE

AHC4539 was used in ELISA to investigate antibodies produced by patients with alcoholic liver disease

Thu Jan 01 00:00:00 EST 2004

Identification of a conserved region of Plasmodium falciparum MSP3 targeted by biologically active antibodies to improve vaccine design.

The Journal of infectious diseases

Singh S,Soe S,Mejia JP,Roussilhon C,Theisen M,Corradin G,Druilhe P

AHC4539 was used in ELISA to investigate epitopes of merozoite surface protein 3

Wed Sep 01 00:00:00 EDT 2004

Insulin-like growth factor-I stimulates IL-10 production in human T cells.

Journal of leukocyte biology

Kooijman R,Coppens A

AHC4539 was used in ELISA to explore the immunomodulatory effects of insulin-like growth factor through regulation of cytokine production

Fri Oct 01 00:00:00 EDT 2004

Bordetella pertussis infection in 2-month-old infants promotes type 1 T cell responses.

Journal of immunology (Baltimore, Md. : 1950)

Mascart F,Verscheure V,Malfroot A,Hainaut M,Piérard D,Temerman S,Peltier A,Debrie AS,Levy J,Del Giudice G,Locht C

Sat Feb 01 00:00:00 EST 2003

Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production.

International immunology

Bullens DM,Rafiq K,Charitidou L,Peng X,Kasran A,Warmerdam PA,Van Gool SW,Ceuppens JL

AHC4539 was used in ELISA to examine the effects of co-stimulation by CD58 on the production of Th1-type or Th2 type cytokines.

Thu Feb 01 00:00:00 EST 2001

Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.

The Journal of experimental medicine

Braun MC,He J,Wu CY,Kelsall BL

AHC4539 was used in ELISA to show that cholera toxin inhibits the production of interleukin-2.

Mon Feb 01 00:00:00 EST 1999

Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.

The Journal of experimental medicine

Braun MC,He J,Wu CY,Kelsall BL

AHC4539 was used in ELISA to show that cholera toxin inhibits the production of interleukin-2.

Mon Feb 01 00:00:00 EST 1999

Bioinformatics

Protein Aliases: H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Immune interferon; Interferon gamma; Interferon y; Interferon γ; interferon, gamma; Interferonγ; M-IFN-g; R-IFN-g

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Gene Aliases: IFG; IFI; IFNG

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UniProt ID: (Human) P01579

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Entrez Gene ID: (Human) 3458

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Function(s)

cytokine activity interferon-gamma receptor binding cytokine interferon superfamily intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IFN gamma Monoclonal Antibody (67F12A8), Biotin

IFN gamma Antibody (AHC4539) in ELISA

Product Details

AHC4539

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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