Leinco Technologies

IFN gamma Monoclonal Antibody (H22), Functional Grade, In Vivo PLATINUM™

View all (214) IFN gamma antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

I-1190-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

Immunohistochemistry (IHC)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-

Neutralization (Neu)

Assay-dependent
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG

Class

Monoclonal

Type

Antibody

Clone

H22

Immunogen

Proprietary immunogen

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

4°C short term, -80°C long term

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Specificity: Armenian Hamster Anti-Mouse Interferon Gamma (IFN-gamma) (Clone H22) recognizes an epitope on Mouse IFN-gamma. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern.

Endotoxin Level: <0.5 EU/mg as determined by the LAL method. Purity: >95% by SDS Page; ≥98% monomer by analytical SEC.

Product Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing: To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8 C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80 C. Avoid Repeated Freeze Thaw Cycles.

This antibody has been tested in the following applications: Western Blotting, Neutralization, Immunoprecipitation, Immunofluorescence, ELISA.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

View more View less

Gene Aliases: Ifg; IFN-g; Ifng

View more View less

UniProt ID: (Mouse) P01580

View more View less

Entrez Gene ID: (Mouse) 15978

View more View less

Function(s)
cytokine activity interferon-gamma receptor binding protein binding cytokine interferon superfamily intercellular signal molecule
Process(es)
protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter neutrophil apoptotic process regulation of the force of heart contraction adaptive immune response CD8-positive, alpha-beta T cell differentiation involved in immune response regulation of transcription, DNA-templated apoptotic process inflammatory cell apoptotic process immune response humoral immune response cell cycle arrest positive regulation of cell proliferation negative regulation of cell proliferation response to virus positive regulation of epithelial cell migration antigen processing and presentation neutrophil chemotaxis negative regulation of epithelial cell differentiation endoplasmic reticulum unfolded protein response negative regulation of myelination positive regulation of synaptic transmission, cholinergic negative regulation of interleukin-17 production positive regulation of interleukin-12 production positive regulation of interleukin-23 production positive regulation of tumor necrosis factor production positive regulation of peptidyl-serine phosphorylation of STAT protein positive regulation of smooth muscle cell apoptotic process regulation of growth positive regulation of T cell proliferation positive regulation of tyrosine phosphorylation of Stat1 protein defense response to bacterium defense response to protozoan negative regulation of growth of symbiont in host negative regulation of growth of symbiont involved in interaction with host positive regulation of chemokine biosynthetic process positive regulation of interleukin-12 biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of nitric oxide biosynthetic process positive regulation of neuron differentiation positive regulation of osteoclast differentiation positive regulation of cell adhesion positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgG isotypes negative regulation of smooth muscle cell proliferation positive regulation of interleukin-1 beta secretion regulation of immune response regulation of insulin secretion sensory perception of mechanical stimulus positive regulation of membrane protein ectodomain proteolysis defense response to virus positive regulation of killing of cells of other organism positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity positive regulation of fructose 1,6-bisphosphate metabolic process positive regulation of vitamin D biosynthetic process positive regulation of calcidiol 1-monooxygenase activity cellular response to lipopolysaccharide cellular response to interleukin-18 extrinsic apoptotic signaling pathway regulation of neuronal action potential positive regulation of tumor necrosis factor (ligand) superfamily member 11 production regulation of hepatocyte proliferation
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support