Invitrogen

IFN gamma Monoclonal Antibody (P2F6)

Product Details

MP700

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 17 publications 17 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Pig

Published species

Human, Pig

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

P2F6

Immunogen

Recombinant porcine IFN gamma

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223615

Product Specific Information

MP700 targets IFN gamma in ELISA, and WB applications and shows reactivity with Porcine samples.

The MP700 immunogen is recombinant porcine IFN gamma.

MP700 detects IFN gamma which has a predicted molecular weight of approximately 17 kDa.

The MP700 porcine IFNg antibody (clone PTF6) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MP701B (biotinylated conjugate of clone P2C11). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Göttingen Minipigs as a Model to Evaluate Longevity, Functionality, and Memory of Immune Response Induced by Pertussis Vaccines.

Frontiers in immunology

Vaure C,Grégoire-Barou V,Courtois V,Chautard E,Dégletagne C,Liu Y

MP700 was used in Fluorophore-linked Immunosorbent Assay to suggest that the Göttingen minipig is an attractive preclinical model to predict the long-term immunogenicity of human vaccines against Bordetella pertussis and potentially also vaccines against other pathogens.

Tue Jun 29 00:00:00 EDT 2021

Expression of T-Bet, Eomesodermin, and GATA-3 Correlates With Distinct Phenotypes and Functional Properties in Porcine γδ T Cells.

Frontiers in immunology

Rodríguez-Gómez IM,Talker SC,Käser T,Stadler M,Reiter L,Ladinig A,Milburn JV,Hammer SE,Mair KH,Saalmüller A,Gerner W

MP700 was used in Fluorophore-linked Immunosorbent Assay to analyse the expression of GATA-3, T-Bet, and eomesodermin in γδ thymocytes, mature γδ T cells from blood, spleen, lymph nodes, lung tissue, and in vitro stimulated γδ T cells.

Tue Jul 14 00:00:00 EDT 2020

Low antigen dose formulated in CAF09 adjuvant Favours a cytotoxic T-cell response following intraperitoneal immunization in Göttingen minipigs.

Vaccine

Overgaard NH,Frøsig TM,Jakobsen JT,Buus S,Andersen MH,Jungersen G

MP700 was used in Enzyme-linked immunosorbent spot to show that the immune system is indeed influenced by the antigen dose; hence underlining the importance of correctly determining which dose to use in order to generate a certain type of immune response.

Mon Oct 09 00:00:00 EDT 2017

A multi-subunit Chlamydia vaccine inducing neutralizing antibodies and strong IFN-γ⁺ CMI responses protects against a genital infection in minipigs.

Immunology and cell biology

Bøje S,Olsen AW,Erneholm K,Agerholm JS,Jungersen G,Andersen P,Follmann F

MP700 was used in ELISA to analyze a multi-subunit Chlamydia vaccine inducing neutralizing antibodies and strong IFN-gamma (+) CMI responses can protect against genital infection in minipigs

Mon Feb 01 00:00:00 EST 2016

Concurrent host-pathogen gene expression in the lungs of pigs challenged with Actinobacillus pleuropneumoniae.

BMC genomics

Brogaard L,Klitgaard K,Heegaard PM,Hansen MS,Jensen TK,Skovgaard K

MP700 was used in ELISA to study the lungs of pigs challenged with Actinobacillus pleuropneumoniae and their concurrent host-pathogen gene expression

Thu May 28 00:00:00 EDT 2015

Time-course of antibody and cell-mediated immune responses to Porcine Reproductive and Respiratory Syndrome virus under field conditions.

Research in veterinary science

Dotti S,Guadagnini G,Salvini F,Razzuoli E,Ferrari M,Alborali GL,Amadori M

MP700 was used in ELISA to follow antibody and cell-mediated immune responses to PRRSV infection in pigs living in a farm environment

Sat Jun 01 00:00:00 EDT 2013

Characterization of the interferon-α response of pigs to the weaning stress.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Razzuoli E,Villa R,Sossi E,Amadori M

MP700 was used in ELISA to investigate the characteristics of interferon alpha response in pigs with early weaning

Tue Feb 01 00:00:00 EST 2011

Co-incubation with IL-18 potentiates antigen-specific IFN-γ response in a whole-blood stimulation assay for measurement of cell-mediated immune responses in pigs experimentally infected with Lawsonia intracellularis.

Veterinary immunology and immunopathology

Riber U,Boesen HT,Jakobsen JT,Nguyen LT,Jungersen G

MP700 was used in ELISA to study the effect of IL-18 on IFN gamma response in a whole-blood stimulation assay

Tue Feb 15 00:00:00 EST 2011

Immunogenicity and protective efficacy of an elastase-dependent live attenuated swine influenza virus vaccine administered intranasally in pigs.

Vaccine

Masic A,Lu X,Li J,Mutwiri GK,Babiuk LA,Brown EG,Zhou Y

MP700 was used in ELISA to characterize an elastase-dependent live attenuated influenza vaccine against swine influenza virus

Fri Oct 08 00:00:00 EDT 2010

Constitutive expression of interferons in swine leukocytes.

Research in veterinary science

Amadori M,Cristiano A,Ferrari M

MP700 was used in immunoprecipitation to investigate the expression of interferons in the white blood cells of pigs

Mon Feb 01 00:00:00 EST 2010

Elective cesarean delivery affects gut maturation and delays microbial colonization but does not increase necrotizing enterocolitis in preterm pigs.

American journal of physiology. Regulatory, integrative and comparative physiology

Siggers RH,Thymann T,Jensen BB,Mølbak L,Heegaard PM,Schmidt M,Buddington RK,Sangild PT

MP700 was used in ELISA to study the effect of cesarean delivery on gut maturation and necrotizing enterocolitis

Sat Mar 01 00:00:00 EST 2008

Identification of immunodominant T-cell epitopes present in glycoprotein 5 of the North American genotype of porcine reproductive and respiratory syndrome virus.

Vaccine

Vashisht K,Goldberg TL,Husmann RJ,Schnitzlein W,Zuckermann FA

Tue Aug 26 00:00:00 EDT 2008

Efficacy and functionality of lipoprotein OprI from Pseudomonas aeruginosa as adjuvant for a subunit vaccine against classical swine fever.

Vaccine

Rau H,Revets H,Cornelis P,Titzmann A,Ruggli N,McCullough KC,Summerfield A

MP700 was used in ELISA to study the role of OprI from P. aeruginosa against classical swine fever

Mon May 29 00:00:00 EDT 2006

Fibrocytes are potent stimulators of anti-virus cytotoxic T cells.

Journal of leukocyte biology

Balmelli C,Ruggli N,McCullough K,Summerfield A

MP700 was used in ELISA to study the activation of anti-virus cytotoxic T cells

Wed Jun 01 00:00:00 EDT 2005

Identification of classical swine fever virus protein E2 as a target for cytotoxic T cells by using mRNA-transfected antigen-presenting cells.

The Journal of general virology

Ceppi M,de Bruin MGM,Seuberlich T,Balmelli C,Pascolo S,Ruggli N,Wienhold D,Tratschin JD,McCullough KC,Summerfield A

MP700 was used in ELISA to study the role of classical swine fever virus protein E2 in cytotoxic T cell effect

Thu Sep 01 00:00:00 EDT 2005

Cytokines and synthetic double-stranded RNA augment the T helper 1 immune response of swine to porcine reproductive and respiratory syndrome virus.

Veterinary immunology and immunopathology

Meier WA,Husmann RJ,Schnitzlein WM,Osorio FA,Lunney JK,Zuckermann FA

Wed Dec 08 00:00:00 EST 2004

Deciphering the involvement of innate immune factors in the development of the host response to PRRSV vaccination.

Veterinary immunology and immunopathology

Royaee AR,Husmann RJ,Dawson HD,Calzada-Nova G,Schnitzlein WM,Zuckermann FA,Lunney JK

Wed Dec 08 00:00:00 EST 2004

Gradual development of the interferon-gamma response of swine to porcine reproductive and respiratory syndrome virus infection or vaccination.

Virology

Meier WA,Galeota J,Osorio FA,Husmann RJ,Schnitzlein WM,Zuckermann FA

Fri Apr 25 00:00:00 EDT 2003

Quantitative detection of porcine interferon-gamma in response to mitogen, superantigen and recall viral antigen.

Veterinary immunology and immunopathology

Mateu de Antonio E,Husmann RJ,Hansen R,Lunney JK,Strom D,Martin S,Zuckermann FA

Fri Feb 27 00:00:00 EST 1998

Bioinformatics

Protein Aliases: cytokine; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

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Gene Aliases: IFNG

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UniProt ID: (Pig) P17803

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Entrez Gene ID: (Pig) 396991

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Function(s)

interferon-gamma receptor binding cytokine interferon superfamily intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IFN gamma Monoclonal Antibody (P2F6)

Product Details

MP700

Western Blot (WB)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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