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Invitrogen
IFNAR2 Monoclonal Antibody (MMHAR-2)
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
IFNAR2 Antibody (213851) in Functional
THP-1 cells were infected with virus in presence of IFNAR2 antibody (Product # 213851, final concentration: 10 µg/mL). Cells were collected at 24 hours post-infection followed by RNA extraction and RT-qPCR. The mRNA level of AIM2, an interferon-inducible protein, was measured by real-time PCR. Data courtesy of Antibody Data Exchange Program.
Product Details
213851
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
MMHAR-2
Immunogen
Human interferon alpha/beta receptor chain 2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ion-exchange chromatography, Size-exclusion chromatography
Storage buffer
PBS with 0.1% BSA
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
RRID
AB_223508
Product Specific Information
This antibody neutralizes human IFN-alpha receptor, interacting with the extracellular domain. It binds to human IFN-alpha receptor with high affinity and blocks biological action of Type I IFNs. To measure bioactivity, interferon was titrated with the use of a cytopathic effect inhibition assay, where one neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha to a 50% endpoint. In this antiviral assay, approximately one unit/mL of interferon was necessary to produce a cytopathic effect of 50%.
Purity is >95% by SDS-PAGE and endotoxin level <1 EU/ug.
Store at -20ºC for up to 6 months. For long-term storage, store at -70ºC for retention of full activity. Thaw product by incubation in cold water and aliquot into separate vials, adding 0.1% BSA for additional stability. Refreeze product using a dry ice or a dry ice/alcohol bath.
Packaged by and distributed for PBL Assay Science
Target Information
The type I interferons, IFN-alpha and IFN-beta, are a group of structurally and functionally related proteins that are induced by either viruses or double-stranded RNA and are defined by their ability to confer an antiviral state in cells. IFN-alpha and IFN-beta appear to compete with one another for binding to a common cell surface receptor, while immune IFN (IFN-gamma) binds to a distinct receptor. This distinct receptor, IFN-alphaR, is only weakly responsive to type I interferons, in contrast to IFN-alpha/betaR, which binds to and responds effectively to IFN-beta and to several of the IFN-alpha subtypes. IFN-alpha/betaR is expressed as two alternatively spliced transcripts, designated IFN-alpha/betaRalpha (IFN-alpha/betaR1) and IFN-alpha/betaRbeta (IFN-alpha/betaR2), both of which are involved in signal transduction and ligand binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: human interferon alpha/beta receptor; IFN alpha/betaRbeta; IFN R2; IFN-alpha/beta receptor 2; IFN-alpha/betaR2; IFN-alpha/betaRbeta; IFN-R-2; IFNalpha/beta R2; IFNalpha/beta-Rbeta; IFNalpha/betaR2; IFNR2; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; Interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2
Gene Aliases: IFN-alpha-REC; IFN-R; IFNABR; IFNAR2; IFNARB; IMD45
UniProt ID: (Human) P48551
Entrez Gene ID: (Human) 3455
regulation of transcription from RNA polymerase II promoter
cell surface receptor signaling pathway
JAK-STAT cascade
cell proliferation
response to virus
response to interferon-alpha
response to interferon-beta
defense response to virus
type I interferon signaling pathway
regulation of type I interferon-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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