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IFT122 Antibody (PA5-148291) in ICC/IF
Immunocytochemistry analysis of IFT122 in A431 cells. Samples were incubated with IFT122 polyclonal antibody (Product # PA5-148291) using a dilution of 1:200 at 4°C overnight followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 10% serum. Counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
Product Details
PA5-148291
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived Human IFT122 fragment
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7
Contains
0.03% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This cytoplasmic protein contains seven WD repeats and an AF-2 domain which function by recruiting coregulatory molecules and in transcriptional activation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: intraflagellar transport 122 homolog; Intraflagellar transport protein 122 homolog; WD repeat domain 10; WD repeat-containing protein 10; WD repeat-containing protein 140
Gene Aliases: CED; CED1; IFT122; SPG; WDR10; WDR10p; WDR140
UniProt ID: (Human) Q9HBG6
Entrez Gene ID: (Human) 55764
neural tube closure
signal transduction downstream of smoothened
embryonic body morphogenesis
negative regulation of smoothened signaling pathway involved in ventral spinal cord patterning
embryonic heart tube development
embryonic forelimb morphogenesis
intraciliary anterograde transport
intraciliary retrograde transport
intraciliary transport involved in cilium morphogenesis
cilium assembly
embryonic digit morphogenesis
negative regulation of smoothened signaling pathway
camera-type eye morphogenesis
negative regulation of epithelial cell proliferation
limb development
cilium morphogenesis
ciliary receptor clustering involved in smoothened signaling pathway
smoothened signaling pathway involved in dorsal/ventral neural tube patterning
embryonic heart tube left/right pattern formation
protein localization to cilium
establishment of protein localization to organelle
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