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IFT25 Antibody (PA5-100040) in ICC/IF
Immunofluorescent analysis of IFT25 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and IFT25 polyclonal antibody (Product # PA5-100040) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100040
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HSPB11(Accession Q9Y547), corresponding to amino acid residues C7-V57.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2815570
Product Specific Information
Antibody detects endogenous levels of total IFT25.
Target Information
HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Heat shock protein; Heat shock protein beta-11; heat shock protein family B (small), member 11; Heat shock protein family B member 11; HSP; Hspb11; Hspb11 heat shock protein family B (small), member 11; intraflagellar transport 25 homolog; Intraflagellar transport protein 25 homolog; Placental protein 25; placental protein 25 homolog; PP25
Gene Aliases: 2900042B11Rik; C1orf41; HSPB11; HSPC034; HSPCO34; IFT25; PP25
UniProt ID: (Human) Q9Y547, (Mouse) Q9D6H2
Entrez Gene ID: (Human) 51668, (Mouse) 72938, (Rat) 685284
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