Invitrogen

IGF1 Polyclonal Antibody, PeproTech®

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

500-P157G-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.5-2.0 µg/mL
-

Neutralization (Neu)

-
View 1 publication 1 publication

Radioimmune Assays (RIA)

-
View 1 publication 1 publication

In vitro Assay (IV)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine IGF-I
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929535

Product Specific Information

AA Sequence of recombinant protein: GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine IGF-I. Anti-Murine IGF-I-specific antibody was purified by affinity chromatography employing an immobilized Murine IGF-I matrix.

Sandwich ELISA: To detect Murine IGF-I by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IGF-I (500-P157GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IGF-I.

Western Blot: To detect Murine IGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: H-IGF-1; IGF; IGF-I; IGF-IA; IGF-IB; IGF1A; IGFIa; Insulin like growth factor; Insulin-like growth factor I; M-IGF-1; OTTHUMP00000195084; R-IGF-1; Somatomedin; Somatomedin C

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Gene Aliases: C730016P09Rik; Igf-1; Igf-I; Igf1

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UniProt ID: (Mouse) P05017

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Entrez Gene ID: (Mouse) 16000

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Function(s)
insulin receptor binding insulin-like growth factor receptor binding integrin binding hormone activity steroid binding protein binding growth factor activity protein serine/threonine kinase activator activity growth factor
Process(es)
cell activation regulation of protein phosphorylation blood vessel remodeling regulation of translation nervous system development memory cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation response to heat glial cell differentiation regulation of gene expression positive regulation of cardiac muscle hypertrophy phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration muscle hypertrophy myotube cell development positive regulation of smooth muscle cell migration positive regulation of cerebellar granule cell precursor proliferation water homeostasis proteoglycan biosynthetic process positive regulation of cell growth lung development positive regulation of cell migration mammary gland development exocrine pancreas development regulation of establishment or maintenance of cell polarity regulation of intracellular steroid hormone receptor signaling pathway positive regulation of protein import into nucleus, translocation negative regulation of smooth muscle cell apoptotic process multicellular organism growth bone mineralization involved in bone maturation positive regulation of activated T cell proliferation regulation of cell proliferation positive regulation of tyrosine phosphorylation of Stat5 protein negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of DNA binding positive regulation of MAPK cascade protein kinase B signaling positive regulation of insulin-like growth factor receptor signaling pathway myoblast differentiation positive regulation of fat cell differentiation positive regulation of osteoblast differentiation positive regulation of glycogen biosynthetic process positive regulation of DNA replication positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of Ras protein signal transduction insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation lung alveolus development cell development positive regulation of smooth muscle cell proliferation branching morphogenesis of an epithelial tube inner ear development chondroitin sulfate proteoglycan biosynthetic process positive regulation of epithelial cell proliferation positive regulation of peptidyl-tyrosine phosphorylation detection of mechanical stimulus involved in sensory perception regulation of protein metabolic process myoblast proliferation positive regulation of protein kinase B signaling regulation of calcium ion transport negative regulation of oocyte development lung vasculature development lung lobe morphogenesis Type I pneumocyte differentiation Type II pneumocyte differentiation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis prostate gland growth prostate gland epithelium morphogenesis prostate gland stromal morphogenesis negative regulation of androgen receptor signaling pathway ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of calcineurin-NFAT signaling cascade positive regulation of protein serine/threonine kinase activity positive regulation of steroid hormone biosynthetic process negative regulation of release of cytochrome c from mitochondria extrinsic apoptotic signaling pathway in absence of ligand negative regulation of neuron death positive regulation of trophectodermal cell proliferation positive regulation of myoblast proliferation negative regulation of extrinsic apoptotic signaling pathway
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