Invitrogen

IGF2R Monoclonal Antibody (2G11)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

15 Published Figures

37 References

View all (23) IGF2R antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

IGF2R Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 21

IGF2R Antibody (MA1-066) in ICC/IF

Immunofluorescence analysis of IGF2R was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IGF2R Monoclonal Antibody (2G11) (Product # MA1-066) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Pr... View More

IGF2R Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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IGF2R Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-IGF2R Monoclonal Antibody (2G11) (Product # MA1-066), shows expression in Hep G2 compared to low expressing A549. {RE}

Product Details

MA1-066

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

Assay-dependent

Immunocytochemistry (ICC/IF)

2-5 µg/mL

View 30 publications 30 publications

Flow Cytometry (Flow)

1 µg / 10^6 cells

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Bovine, Human, Non-human primate, Rat

Published species

Bovine, Hamster, Human, Non-human primate, Rabbit, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2G11

Immunogen

Purified bovine ~300 kDa CI-MPR.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2264554

Product Specific Information

MA1-066 detects mannose 6-phospate receptor (MPR) from human, rat, monkey and bovine tissues. This antibody does not detect Chinese hamster ovary cell MPR.

MA1-066 has been successfully used in Western blot, immunofluorescence, EM immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a ~300 kDa protein representing MPR in HeLa cell extract under non-reducing conditions. Immunofluorescence staining of MPR in HeLa cells with MA1-066 results in perinuclear staining.

MA1-066 immunogen is purified bovine ~300 kDa Cl-MPR. This antibody is shown to recognize an epitope in the extracellular domain of MPR.

Target Information

Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of approximately 300 kDa cation independent mannose 6-phospate receptors (CI-MPR). The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPR's is predominantly within late endosomes, fewer in the trans Golgi network and approximately 10 % at cell surface. In addition to its man6P binding activity, the MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An approximately 240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Distinct changes in endosomal composition promote NLRP3 inflammasome activation.

Nature immunology

Zhang Z,Venditti R,Ran L,Liu Z,Vivot K,Schürmann A,Bonifacino JS,De Matteis MA,Ricci R

MA1-066 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (PFA fixed), Immunoprecipitation, Western Blot to demonstrate that NLRP3 inflammasome activators primarily converge on disruption of endoplasmic reticulum-endosome membrane contact sites (EECS).

Sun Jan 01 00:00:00 EST 2023

Coronin 1C restricts endosomal branched actin to organize ER contact and endosome fission.

The Journal of cell biology

Striepen JF,Voeltz GK

Published figure using IGF2R monoclonal antibody (Product # MA1-066) in Immunocytochemistry

Mon Aug 01 00:00:00 EDT 2022

Coronin 1C restricts endosomal branched actin to organize ER contact and endosome fission.

The Journal of cell biology

Striepen JF,Voeltz GK

Published figure using IGF2R monoclonal antibody (Product # MA1-066) in Immunocytochemistry

Mon Aug 01 00:00:00 EDT 2022

Fate of the Fc fusion protein aflibercept in retinal endothelial cells: competition of recycling and degradation.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie

Deissler HL,Lang GK,Lang GE

Published figure using IGF2R monoclonal antibody (Product # MA1-066) in Immunofluorescence

Tue Jan 01 00:00:00 EST 2019

A Novel Class of ER Membrane Proteins Regulates ER-Associated Endosome Fission.

Cell

Hoyer MJ,Chitwood PJ,Ebmeier CC,Striepen JF,Qi RZ,Old WM,Voeltz GK

Published figure using IGF2R monoclonal antibody (Product # MA1-066) in Immunocytochemistry

Thu Sep 20 00:00:00 EDT 2018

Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5.

Nature communications

Shi M,Chen B,Mahajan D,Boh BK,Zhou Y,Dutta B,Tie HC,Sze SK,Wu G,Lu L

MA1-066 was used in Immunocytochemistry-immunoflourescence to demonstrate that SLC38A9, v-ATPase and Ragulator, but not Rag GTPases and mTORC1, are essential for amino acid-stimulated trafficking.

Mon Nov 26 00:00:00 EST 2018

Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.

The Journal of cell biology

Simonetti B,Danson CM,Heesom KJ,Cullen PJ

Published figure using IGF2R monoclonal antibody (Product # MA1-066) in Immunofluorescence

Mon Nov 06 00:00:00 EST 2017

Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.

The Journal of cell biology

Simonetti B,Danson CM,Heesom KJ,Cullen PJ

Published figure using IGF2R monoclonal antibody (Product # MA1-066) in Immunofluorescence

Mon Nov 06 00:00:00 EST 2017

Caspase-mediated proteolysis of the sorting nexin 2 disrupts retromer assembly and potentiates Met/hepatocyte growth factor receptor signaling.

Cell death discovery

Duclos CM,Champagne A,Carrier JC,Saucier C,Lavoie CL,Denault JB

MA1066 was used in immunocytochemistry and western blot to demonstrate that sorting nexin 1 and 2 are cleaved by initiator caspases during apoptosis

Wed Sep 30 00:00:00 EDT 2020

Excess sphingomyelin disturbs ATG9A trafficking and autophagosome closure.

Autophagy

Corcelle-Termeau E,Vindeløv SD,Hämälistö S,Mograbi B,Keldsbo A,Bräsen JH,Favaro E,Adam D,Szyniarowski P,Hofman P,Krautwald S,Farkas T,Petersen NH,Rohde M,Linkermann A,Jäättelä M

MA1-066 was used in immunocytochemistry to study disruption of ATG9A trafficking and autophagosome closure by excess sphinogomyelin

Tue May 03 00:00:00 EDT 2016

A Trematode Parasite Derived Growth Factor Binds and Exerts Influences on Host Immune Functions via Host Cytokine Receptor Complexes.

PLoS pathogens

Sulaiman AA,Zolnierczyk K,Japa O,Owen JP,Maddison BC,Emes RD,Hodgkinson JE,Gough KC,Flynn RJ

MA1066 was used in immunohistochemistry to investigate the effect of Fasciola hepatica FhTLM on host immune functions

Tue Nov 01 00:00:00 EDT 2016

Syntaxin 5-dependent retrograde transport to the trans-Golgi network is required for adeno-associated virus transduction.

Journal of virology

Nonnenmacher ME,Cintrat JC,Gillet D,Weber T

MA1-066 was used in immunocytochemistry to assess adeno-associated virus transduction and its requirement for syntaxin 5-dependent retrograde transport to the trans-Golgi network

Sun Feb 01 00:00:00 EST 2015

Human Copper Chaperone Atox1 Translocates to the Nucleus but does not Bind DNA In Vitro.

Protein and peptide letters

Kahra D,Mondol T,Niemiec MS,Wittung-Stafshede P

MA1-066 was used in immunocytochemistry to study the nuclear translocation of Atox1.

Mon Feb 22 00:00:00 EST 2016

Chaperonin-containing TCP-1 complex directly binds to the cytoplasmic domain of the LOX-1 receptor.

FEBS letters

Bakthavatsalam D,Soung RH,Tweardy DJ,Chiu W,Dixon RA,Woodside DG

MA1-066 was used in immunocytochemistry to study the interaction between CCT and LOX-1

Fri Jun 13 00:00:00 EDT 2014

EGFR-dependent phosphorylation of leucine-rich repeat kinase LRRK1 is important for proper endosomal trafficking of EGFR.

Molecular biology of the cell

Ishikawa K,Nara A,Matsumoto K,Hanafusa H

MA1-066 was used in immunocytochemistry to study the role of LRRK1 phosphorylation by EGFR in the correct endosomal trafficking of EGFR

Sun Apr 01 00:00:00 EDT 2012

Endo-lysosomal vesicles positive for Rab7 and LAMP1 are terminal vesicles for the transport of dextran.

PloS one

Humphries WH,Szymanski CJ,Payne CK

MA1-066 was used in immunocytochemistry and immunocytochemistry to investigate the roles of endo-lysosomal vesicles in the transport of dextran

Tue Feb 28 00:00:00 EST 2012

Endo-lysosomal vesicles positive for Rab7 and LAMP1 are terminal vesicles for the transport of dextran.

PloS one

Humphries WH,Szymanski CJ,Payne CK

MA1-066 was used in immunocytochemistry and immunocytochemistry to investigate the roles of endo-lysosomal vesicles in the transport of dextran

Tue Feb 28 00:00:00 EST 2012

A spatio-temporal analysis of matrix protein and nucleocapsid trafficking during vesicular stomatitis virus uncoating.

PLoS pathogens

Mire CE,White JM,Whitt MA

MA1-066 was used in immunocytochemistry to investigate the spatio-temporal trafficking of matrix protein and nucleocapsid during vesicular stomatitis virus uncoating

Thu Jul 15 00:00:00 EDT 2010

Intracellular degradation of low-density lipoprotein probed with two-color fluorescence microscopy.

Integrative biology : quantitative biosciences from nano to macro

Humphries WH,Fay NC,Payne CK

MA1-066 was used in immunocytochemistry to study the intracellular degradation of low-density lipoprotein using two-color fluorescence microscopy

Fri Oct 01 00:00:00 EDT 2010

EHD3 regulates early-endosome-to-Golgi transport and preserves Golgi morphology.

Journal of cell science

Naslavsky N,McKenzie J,Altan-Bonnet N,Sheff D,Caplan S

MA1-066 was used in immunocytochemistry to study the role of EHD3 in the regulation of endosome-to-Golgi transport

Sun Feb 01 00:00:00 EST 2009

Myosin Vc is a molecular motor that functions in secretory granule trafficking.

Molecular biology of the cell

Jacobs DT,Weigert R,Grode KD,Donaldson JG,Cheney RE

MA1-066 was used in immunocytochemistry to study the molecular motor role of myosin Vc in secretory granule trafficking

Sun Nov 01 00:00:00 EDT 2009

Modulatory role of phosphoinositide 3-kinase in gastric acid secretion.

American journal of physiology. Gastrointestinal and liver physiology

Mettler SE,Ghayouri S,Christensen GP,Forte JG

MA1-066 was used in western blot to investigate the role of phosphoinositide 3-kinase in gastric acid secretion

Sat Sep 01 00:00:00 EDT 2007

Identification of compartments involved in mammalian subcellular trafficking pathways by indirect immunofluorescence.

Methods in molecular medicine

Doody A,Putnam D

MA1-066 was used in immunocytochemistry to evaluate methods for the compartment identification in subcellular trafficking

Wed Oct 11 00:00:00 EDT 2006

Lowe syndrome protein OCRL1 interacts with clathrin and regulates protein trafficking between endosomes and the trans-Golgi network.

Molecular biology of the cell

Choudhury R,Diao A,Zhang F,Eisenberg E,Saint-Pol A,Williams C,Konstantakopoulos A,Lucocq J,Johannes L,Rabouille C,Greene LE,Lowe M

MA1-066 was used in immunocytochemistry to study the cellular function of hosphatidylinositol 4,5-bisphosphate 5-phosphatase OCRL1.

Mon Aug 01 00:00:00 EDT 2005

A novel GTPase-activating protein for ARF6 directly interacts with clathrin and regulates clathrin-dependent endocytosis.

Molecular biology of the cell

Tanabe K,Torii T,Natsume W,Braesch-Andersen S,Watanabe T,Satake M

MA1-066 was used in immunocytochemistry to study SMAP1, a novel GTPase-activating protein for Arf6 that directly binds clathrin and modulates clathrin-mediated endocytosis

Fri Apr 01 00:00:00 EST 2005

A novel kinesin-like protein, KIF1Bbeta3 is involved in the movement of lysosomes to the cell periphery in non-neuronal cells.

Traffic (Copenhagen, Denmark)

Matsushita M,Tanaka S,Nakamura N,Inoue H,Kanazawa H

MA1-066 was used in Immunocytochemistry-immunoflourescence to indicate that KIF1Bbeta3 is involved in the translocation of lysosomes from perinuclear regions to the cell periphery.

Mon Mar 01 00:00:00 EST 2004

Intracellular processing and activation of membrane type 1 matrix metalloprotease depends on its partitioning into lipid domains.

Journal of cell science

Mazzone M,Baldassarre M,Beznoussenko G,Giacchetti G,Cao J,Zucker S,Luini A,Buccione R

MA1-066 was used in immunocytochemistry to investigate the mechanism of integral membrane type 1 matrix metalloprotease (MT1-MMP) activities.

Wed Dec 15 00:00:00 EST 2004

High-pressure freezing of isolated gastric glands provides new insight into the fine structure and subcellular localization of H+/K+-ATPase in gastric parietal cells.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Sawaguchi A,McDonald KL,Forte JG

MA1-066 was used in immunohistochemistry to show the subcellular localization of hydrogen/potassium-ATPase in gastric parietal cells

Thu Jan 01 00:00:00 EST 2004

Distribution and trafficking of MPR300 is normal in cells with cholesterol accumulated in late endocytic compartments: evidence for early endosome-to-TGN trafficking of MPR300.

Journal of lipid research

Umeda A,Fujita H,Kuronita T,Hirosako K,Himeno M,Tanaka Y

Published figure using IGF2R monoclonal antibody (Product # MA1-066) in Immunocytochemistry

Wed Oct 01 00:00:00 EDT 2003

A role for GRIP domain proteins and/or their ligands in structure and function of the trans Golgi network.

Journal of cell science

Yoshino A,Bieler BM,Harper DC,Cowan DA,Sutterwala S,Gay DM,Cole NB,McCaffery JM,Marks MS

MA1-066 was used in immunocytochemistry to study the functions of GRIP proteins or their ligands in the maintenance of trans Golgi network integrity.

Sat Nov 01 00:00:00 EST 2003

Mechanism of constitutive export from the golgi: bulk flow via the formation, protrusion, and en bloc cleavage of large trans-golgi network tubular domains.

Molecular biology of the cell

Polishchuk EV,Di Pentima A,Luini A,Polishchuk RS

MA1-066 was used in immunohistochemistry to investigate constitutive Golgi complex export process .

Sat Nov 01 00:00:00 EST 2003

Accumulation of tyrosinase in the endolysosomal compartment is induced by U18666A.

Pigment cell research

Hall AM,Krishnamoorthy L,Orlow SJ

MA1-066 was used in immunocytochemistry to study the accumulation of tyrosinase in an endolysosomal compartment of melanocytes treated with 3-beta-(2-diethylamonoethoxy)-androstenone

Tue Apr 01 00:00:00 EST 2003

Human myosin-Vc is a novel class V myosin expressed in epithelial cells.

Journal of cell science

Rodriguez OC,Cheney RE

MA1-066 was used in immunocytochemistry to investigate the role of myosin-Vc as a novel class V myosin.

Fri Mar 01 00:00:00 EST 2002

Overexpression of an ADP-ribosylation factor-guanine nucleotide exchange factor, BIG2, uncouples brefeldin A-induced adaptor protein-1 coat dissociation and membrane tubulation.

The Journal of biological chemistry

Shinotsuka C,Yoshida Y,Kawamoto K,Takatsu H,Nakayama K

MA1-066 was used in immunocytochemistry to investigate the role of BIG2 during the membrane association of AP-1.

Fri Mar 15 00:00:00 EST 2002

Sorting of mannose 6-phosphate receptors mediated by the GGAs.

Science (New York, N.Y.)

Puertollano R,Aguilar RC,Gorshkova I,Crouch RJ,Bonifacino JS

MA1-066 was used in immunocytochemistry to study the role of Golgi-localized and ARF-binding proteins in mannose 6-phosphate receptor sorting.

Fri Jun 01 00:00:00 EDT 2001

Functionally distinct pools of actin in secretory cells.

American journal of physiology. Cell physiology

Ammar DA,Nguyen PN,Forte JG

MA1-066 was used in immunocytochemistry to investigate the importance of cytoskeleton in the secretion of HCl in stomach

Wed Aug 01 00:00:00 EDT 2001

Expression of rab11a N124I in gastric parietal cells inhibits stimulatory recruitment of the H+-K+-ATPase.

The American journal of physiology

Duman JG,Tyagarajan K,Kolsi MS,Moore HP,Forte JG

MA1-066 was used in immunocytochemistry to investigate the role of RAB11a N124I for stimulatory recruitment of the hydrogen-potassium-ATPase in gastric parietal cells

Wed Sep 01 00:00:00 EDT 1999

Receptor extracellular domains may contain trafficking information. Studies of the 300-kDa mannose 6-phosphate receptor.

The Journal of biological chemistry

Dintzis SM,Velculescu VE,Pfeffer SR

MA1-066 was used in immunocytochemistry to study the role of receptor extracellular domains in protein trafficking

Fri Apr 22 00:00:00 EDT 1994

Serum form of the rat insulin-like growth factor II/mannose 6-phosphate receptor is truncated in the carboxyl-terminal domain.

The Journal of biological chemistry

MacDonald RG,Tepper MA,Clairmont KB,Perregaux SB,Czech MP

MA1-066 was used in western blot to investigate the proteolysis of mannose 6-phosphate receptor protein in rat serum

Sat Feb 25 00:00:00 EST 1989

Bioinformatics

Protein Aliases: 300 kDa mannose 6-phosphate receptor; cation-independent mannose 6-phosphate receptor protein; Cation-independent mannose-6-phosphate receptor; CD222; CI Man-6-P receptor; IGF-II receptor; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; insulin-like growth factor receptor type 2; M6P/IGF2 receptor; M6P/IGF2R; M6PR; MPR 300

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Gene Aliases: CD222; CI-M6PR; CI-MPR; CIMPR; IGF2R; Igfr2; M6P; M6P-R; M6P/IGF2R; MPR 300; MPR1; MPR300; MPRI

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UniProt ID: (Human) P11717, (Bovine) P08169

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Entrez Gene ID: (Human) 3482, (Bovine) 281849, (Rat) 25151

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Function(s)

glycoprotein binding G-protein alpha-subunit binding retinoic acid binding receptor activity G-protein coupled receptor activity insulin-like growth factor-activated receptor activity transporter activity protein binding mannose binding enzyme binding insulin-like growth factor II binding identical protein binding phosphoprotein binding insulin-like growth factor binding kringle domain binding signaling receptor activity membrane traffic protein

Process(es)

liver development receptor-mediated endocytosis signal transduction G-protein coupled receptor signaling pathway spermatogenesis post-embryonic development organ regeneration response to retinoic acid positive regulation of apoptotic process insulin-like growth factor receptor signaling pathway lysosomal transport protein transport regulation of apoptotic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IGF2R Monoclonal Antibody (2G11)

IGF2R Antibody (MA1-066) in ICC/IF

Product Details

MA1-066

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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