Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
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AA Sequence of recombinant protein: MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-BP1. Anti-Human IGF-BP1-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hIGF-BP1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IGF-BP1 (500-P228) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIGF-BP1.
Western Blot: To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIGF-BP1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P228BT-1MG will be provided as 2 x 500 µg
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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