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IGFBP-1 Antibody (500-P228BT-25UG) in ELISA
Sandwich ELISA: To detect hIGF-BP1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of IGFBP-1 Polyclonal Antibody, Biotin (Product # 500-P228BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech IGFBP-1 Polyclonal Antibody (Product # 500-P228-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIGF-BP1.
Product Details
500-P228BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IGF-BP1
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929922
Product Specific Information
AA Sequence of recombinant protein: MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-BP1. Anti-Human IGF-BP1-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hIGF-BP1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IGF-BP1 (500-P228) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIGF-BP1.
Western Blot: To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIGF-BP1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P228BT-1MG will be provided as 2 x 500 µg
Target Information
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; Binding protein 25; Binding protein 26; Binding protein 28; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; hIGFBP 1; hIGFBP1; IBP 1; IBP-1; IGF BP25; IGF-binding protein 1; IGFBP; IGFBP 1; IGFBP-1; Insulin like growth factor binding protein; insulin-like growth factor binding protein 1; Insulin-like growth factor-binding protein 1; Placental protein 12; PP 12; PP12
Gene Aliases: AFBP; hIGFBP-1; IBP1; IGF-BP25; IGFBP1; PP12
UniProt ID: (Human) P08833
Entrez Gene ID: (Human) 3484
signal transduction
insulin receptor signaling pathway
positive regulation of cell growth
PERK-mediated unfolded protein response
tissue regeneration
regulation of insulin-like growth factor receptor signaling pathway
cellular protein metabolic process
negative regulation of canonical Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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