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IGHA2 Antibody (PA5-144322) in ICC/IF
Immunocytochemistry analysis of HepG2 cells with IGHA2 polyclonal antibody (Product # PA5-144322) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Product Details
PA5-144322
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Immunoglobulin heavy constant alpha 2 protein (36-111AA)
Conjugate
Form
Liquid
Concentration
3.55 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_3091475
Target Information
The Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. The human alpha 1 and two allelic alpha 2 immunoglobulin heavy chain constant region genes contain three exons, each encoding a single constant region protein domain. Alignment of the alpha 1 and alpha 2 gene sequences reveals an unusual coupled deletion-duplication in the 5'-flanking region, which can be explained in terms of a slipped-strand mispairing model. Comparison of nucleotide sequences of the alpha 1 gene and two alleles of the alpha 2 gene indicates a localized transfer of genetic information from the 3' end of the alpha 1 gene to one of the alpha 2 alleles, probably by a gene conversion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ig alpha-2 chain C region; Ig alpha-2 chain C region BUT; Ig alpha-2 chain C region LAN; IGHA2Immunoglobulin heavy constant alpha 2
Entrez Gene ID: (Human) 3494
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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