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IKAROS Antibody (PA5-85570) in WB
Western blot was performed using Anti-IKAROS Polyclonal Antibody (Product # PA5-85570) and 60, 52 and 40 k Da bands corresponding to IKZF1 along with an uncharacterized band (*) was observed across positive cell lines (REH, Daudi, Ramos and Jurkat). Whole cell extracts (30 µg lysate) of REH (Lane 1), Daudi (Lane 2), Ramos (Lane 3), Jurkat (Lane 4), U-937 (Lane 5), HL-60 (Lane 6), SH-SY5Y (Lane 7) and A-431 (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred ont... View More
Product Details
PA5-85570
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Ikaros.
Conjugate
Form
Liquid
Concentration
0.38 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792710
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (94%), Chicken (81%), Bovine (88%).
Positive Control: Jurkat, Raji, NCI-H929.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
DNA-binding protein IKAROS functions in the specification and the maturation of the T lymphocyte. It also interacts with a critical control element in the terminal deoxynucleotidyl transferase promoter as well as with the promoters for other genes expressed during early stages of B-cell and T-cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CLL-associated antigen KW-6; DNA-binding protein Ikaros; hIk-1; IKAROS family zinc finger 1 (Ikaros); Ikaros family zinc finger protein 1; Lymphoid transcription factor LyF-1; protein phosphatase 1, regulatory subunit 92; zinc finger protein, subfamily 1A, 1 (Ikaros)
Gene Aliases: CVID13; Hs.54452; IK1; IKAROS; IKZF1; LyF-1; LYF1; PPP1R92; PRO0758; ZNFN1A1
UniProt ID: (Human) Q13422
Entrez Gene ID: (Human) 10320
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
cell cycle
mesoderm development
covalent chromatin modification
lymphocyte differentiation
erythrocyte differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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