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IKAROS Antibody (MA5-28612) in Flow
Flow Cytometry analysis of IKAROS using IKAROS Monoclonal Antibody (4E9), PE (Product # MA5-28612). Separation of human monocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-Ikaros (4E9) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Product Details
MA5-28612
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4E9
Immunogen
Recombinant human Ikaros (C-terminal region)
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745571
Product Specific Information
This antibody recognizes Ikaros, a transcription factor (intracellular antigen) expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.
Recommended dilution is for 100 µL of whole blood or 1x10^6 cells in suspension.
Target Information
DNA-binding protein IKAROS functions in the specification and the maturation of the T lymphocyte. It also interacts with a critical control element in the terminal deoxynucleotidyl transferase promoter as well as with the promoters for other genes expressed during early stages of B-cell and T-cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CLL-associated antigen KW-6; DNA-binding protein Ikaros; hIk-1; IKAROS family zinc finger 1 (Ikaros); Ikaros family zinc finger protein 1; Lymphoid transcription factor LyF-1; protein phosphatase 1, regulatory subunit 92; zinc finger protein, subfamily 1A, 1 (Ikaros)
Gene Aliases: 5832432G11Rik; CVID13; hlk-1; Hs.54452; IK1; IKAROS; IKZF1; LyF-1; LYF1; mKIAA4227; PPP1R92; PRO0758; Zfpn1a1; ZNFN1A1
UniProt ID: (Human) Q13422
Entrez Gene ID: (Human) 10320, (Mouse) 22778
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
cell cycle
mesoderm development
covalent chromatin modification
lymphocyte differentiation
erythrocyte differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
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