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Invitrogen
IKK gamma Monoclonal Antibody (H.256.9)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
IKK gamma Antibody (MA5-15155) in ICC/IF
Immunofluorescence analysis of IKK gamma was performed using HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IKK gamma Mouse Monoclonal Antibody (Product # MA5-15155) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then l... View More
Product Details
MA5-15155
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
H.256.9
Immunogen
Full length human GST-IKK gamma protein.
Conjugate
Form
Liquid
Concentration
2.7 mg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_10977400
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
IKK gamma is the regulatory subunit of the inhibitor of the kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FIP-3; gikk; I-kappa-B kinase gamma; I-kappa-B kinase subunit gamma; ikB kinase subunit gamma; IkB kinase-associated protein 1; IKK-gamma; IKK3; IKKAP1; IKKG; incontinentia pigmenti; inhibitor of kappaB kinase gamma; Inhibitor of nuclear factor kappa-B kinase subunit gamma; mF; NEMO; NF-kappa B essential modulator; NF-kappa-B essential modifier; NF-kappa-B essential modulator; OTTHUMP00000207079; OTTHUMP00000212188
Gene Aliases: AMCBX1; FIP-3; FIP3; Fip3p; IKBKG; IKK-gamma; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; ZC2HC9
UniProt ID: (Human) Q9Y6K9, (Rat) Q6TMG5
Entrez Gene ID: (Human) 8517, (Rat) 309295
signal transducer activity
protein binding
protein domain specific binding
ubiquitin protein ligase binding
protein homodimerization activity
peroxisome proliferator activated receptor binding
metal ion binding
protein heterodimerization activity
K63-linked polyubiquitin binding
linear polyubiquitin binding
activation of MAPK activity
stimulatory C-type lectin receptor signaling pathway
transcription, DNA-templated
apoptotic process
inflammatory response
immune response
cellular response to DNA damage stimulus
I-kappaB kinase/NF-kappaB signaling
JNK cascade
response to virus
regulation of tumor necrosis factor-mediated signaling pathway
TRIF-dependent toll-like receptor signaling pathway
Fc-epsilon receptor signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
positive regulation of transcription from RNA polymerase II promoter
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
stress-activated MAPK cascade
establishment of vesicle localization
nucleotide-binding oligomerization domain containing signaling pathway
negative regulation of neuron death
B cell homeostasis
activation of NF-kappaB-inducing kinase activity
NF-kappaB import into nucleus
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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