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IKZF4 Antibody (PA5-142535) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of IKZF4 in U2OS cells using IKZF4 Polyclonal Antibody (Product # PA5-142535). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-142535
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-RPTFIDRLANSLTKR.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2933182
Product Specific Information
Additional Information: This antibody is expected to recognise reported isoforms NP_071910.3; NP_001338019.1; NP_001338020.1; NP_001338021.1.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:2,000.
Target Information
DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: IKAROS family zinc finger 4 (Eos); Ikaros family zinc finger protein 4; Zinc finger protein Eos; zinc finger protein, subfamily 1A, 4 (Eos); zinc finger transcription factor Eos
Gene Aliases: A630026H08Rik; EOS; IKZF4; KIAA1782; Zfpn1a4; ZNFN1A4
UniProt ID: (Human) Q9H2S9, (Mouse) Q8C208
Entrez Gene ID: (Human) 64375, (Mouse) 22781
transcription factor activity, sequence-specific DNA binding
protein homodimerization activity
bHLH transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
protein heterodimerization activity
nucleic acid binding
DNA binding
protein binding
C2H2 zinc finger transcription factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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