Proteintech

IL 1 alpha Polyclonal Antibody

IL 1 alpha Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

IL 1 alpha Antibody (16765-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed RAW 264.7 cells using 16765-1-AP (IL 1 alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

IL 1 alpha Antibody in Western Blot (WB)

Product Image Gallery

Product Details

16765-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

ELISA (ELISA)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IL 1 alpha Fusion Protein Ag10339 (1-271 aa encoded by BC013142)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAKVPDMFE DLKNCYSENE EDSSSIDHLS LNQKSFYHVS YGPLHEGCMD QSVSLSISET SKTSKLTFKE SMVVVATNGK VLKKRRLSLS QSITDDDLEA IANDSEEEII KPRSAPFSFL SNVKYNFMRI IKYEFILNDA LNQSIIRAND QYLTAAALHN LDEAVKFDMG AYKSSKDDAK ITVILRISKT QLYVTAQDED QPVLLKEMPE IPKTITGSET NLLFFWETHG TKNYFTSVAH PNLFIATKQD YWVCLAGGPP SITDFQILEN QA (1-271 aa encoded by BC013142)

Target Information

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A stress-induced cilium-to-PML-NB route drives senescence initiation.

Nature communications

Ma X,Zhang Y,Zhang Y,Zhang X,Huang Y,He K,Chen C,Hao J,Zhao D,LeBrasseur NK,Kirkland JL,Chini EN,Wei Q,Ling K,Hu J

Mon Apr 03 00:00:00 EDT 2023

EEF1A2 accelerates the protein translation of chemokine in rat myocardial cells induced by ischemia-reperfusion.

Heliyon

Zhang ZJ,Sun ZX,Liu HJ

Sat Apr 01 00:00:00 EDT 2023

HSF1 Alleviates Brain Injury by Inhibiting NLRP3-Induced Pyroptosis in a Sepsis Model.

Mediators of inflammation

He YF,Hu XM,Khan MA,Yu BY,Sheng YC,Xiao XZ,Wan XX,Tan SP,Xiong K

Thu Feb 09 00:00:00 EST 2023

Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation.

Frontiers in pharmacology

Wei Y,Wang K,Zhang Y,Duan Y,Tian Y,Yin H,Fu X,Ma Z,Zhou J,Yu M,Ni Q,Tang W

Tue Apr 04 00:00:00 EDT 2023

m⁶A methylation-induced NR1D1 ablation disrupts the HSC circadian clock and promotes hepatic fibrosis.

Pharmacological research

Chen L,Xia S,Wang F,Zhou Y,Wang S,Yang T,Li Y,Xu M,Zhou Y,Kong D,Zhang Z,Shao J,Xu X,Zhang F,Zheng S

Wed Mar 01 00:00:00 EST 2023

Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway.

Gut microbes

Zhang JW,Zhang D,Yin HS,Zhang H,Hong KQ,Yuan JP,Yu BP

Thu Apr 06 00:00:00 EDT 2023

Early brainstem hemorrhage progression: multi-sequence magnetic resonance imaging and histopathology.

Neural regeneration research

Guo X,Xu JK,Qi X,Wei Y,Wang CW,Li H,Ma L,You C,Tian M

Sun Jan 01 00:00:00 EST 2023

Systemic treatment with GnRH agonist produces antidepressant-like effects in LPS induced depression male mouse model.

Neuropharmacology

Xu Y,Li W,Shi M,Xu X,Guo D,Liu Z,Chen L,Zhong X,Cao W

Tue Aug 01 00:00:00 EDT 2023

Neuro death through autophagy via the acetylation of FoxO1 by SIRT2 in the hippocampus of mice in a autism spectrum disorder mice model.

Journal of cellular physiology

Guo L,Jiang ZM,Zhan YJ,Pan W,Wu QW,Song FX,Zhou X,Zhou XY,Liu LJ,Wang JT

Thu Jun 01 00:00:00 EDT 2023

Celecoxib, Beyond Anti-inflammation, Alleviates Tendon-Derived Stem Cell Senescence in Degenerative Rotator Cuff Tendinopathy.

The American journal of sports medicine

Cai Z,Zhang Y,Liu S,Liu X

Fri Jul 01 00:00:00 EDT 2022

Oridonin Delays Aging Through the AKT Signaling Pathway.

Frontiers in pharmacology

An Y,Zhu J,Wang X,Sun X,Luo C,Zhang Y,Ye Y,Li X,Abulizi A,Huang Z,Zhang H,Yang B,Xie Z

Sat Jul 16 00:00:00 EDT 2022

Grossamide attenuates inflammation by balancing macrophage polarization through metabolic reprogramming of macrophages in mice.

International immunopharmacology

Zhao W,Xu D,Hong W,Zhang L,Wu Q,Gao M,Wang J

Tue Nov 01 00:00:00 EDT 2022

Senescence as a dictator of patient outcomes and therapeutic efficacies in human gastric cancer.

Cell death discovery

Zhou L,Niu Z,Wang Y,Zheng Y,Zhu Y,Wang C,Gao X,Gao L,Zhang W,Zhang K,Melino G,Huang H,Wang X,Sun Q

Mon Jan 10 00:00:00 EST 2022

LipoxinA₄ analog BML-111 protects podocytes cultured in high-glucose medium against oxidative injury via activating Nrf2 pathway.

International immunopharmacology

Hao H,Xie F,Xu F,Wang Q,Wu Y,Zhang D

Sat Oct 01 00:00:00 EDT 2022

LipoxinA₄ analog BML-111 protects podocytes cultured in high-glucose medium against oxidative injury via activating Nrf2 pathway.

International immunopharmacology

Hao H,Xie F,Xu F,Wang Q,Wu Y,Zhang D

Sat Oct 01 00:00:00 EDT 2022

Plasma Exosomes Transfer miR-885-3p Targeting the AKT/NFκB Signaling Pathway to Improve the Sensitivity of Intravenous Glucocorticoid Therapy Against Graves Ophthalmopathy.

Frontiers in immunology

Sun J,Wei J,Zhang Y,Li J,Li J,Yan J,Guo M,Han J,Qiao H

Mon May 02 00:00:00 EDT 2022

L-Se-methylselenocysteine loaded mucoadhesive thermogel for effective treatment of Vulvar candidiasis.

International journal of pharmaceutics

Yang X,Wang M,Kang X,Mo F,Si P,Ma J,Zhang P,Zheng S,Li J,Wang Y,Li Q,Zhang J

Sat Jun 25 00:00:00 EDT 2022

Activation of glucagon-like peptide-1 receptor in microglia attenuates neuroinflammation-induced glial scarring via rescuing Arf and Rho GAP adapter protein 3 expressions after nerve injury.

International journal of biological sciences

Qian Z,Chen H,Xia M,Chang J,Li X,Ye S,Wu S,Jiang S,Bao J,Wang B,Kong R,Zhang S,Zheng S,Cao X,Hong X

Fri Apr 15 00:00:00 EDT 2022

Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Bioactive materials

Luo L,Gong J,Wang Z,Liu Y,Cao J,Qin J,Zuo R,Zhang H,Wang S,Zhao P,Yang D,Zhang M,Wang Y,Zhang J,Zhou Y,Li C,Ni B,Tian Z,Liu M

Thu Sep 01 00:00:00 EDT 2022

Heat Stress Modulates a Placental Immune Response Associated With Alterations in the Development of the Fetal Intestine and Its Innate Immune System in Late Pregnant Mouse.

Frontiers in physiology

Guo H,Liu R,He J,Yao W,Zheng W

Sat Jul 16 00:00:00 EDT 2022

Methylprednisolone Induces Neuro-Protective Effects via the Inhibition of A1 Astrocyte Activation in Traumatic Spinal Cord Injury Mouse Models.

Frontiers in neuroscience

Zou HJ,Guo SW,Zhu L,Xu X,Liu JB

Sat Jun 19 00:00:00 EDT 2021

Gancaonin N from Glycyrrhiza uralensis Attenuates the Inflammatory Response by Downregulating the NF-κB/MAPK Pathway on an Acute Pneumonia In Vitro Model.

Pharmaceutics

Ko HM,Lee SH,Jee W,Jung JH,Kim KI,Jung HJ,Jang HJ

Tue Jul 06 00:00:00 EDT 2021

FOXO4-DRI alleviates age-related testosterone secretion insufficiency by targeting senescent Leydig cells in aged mice.

Aging

Zhang C,Xie Y,Chen H,Lv L,Yao J,Zhang M,Xia K,Feng X,Li Y,Liang X,Sun X,Deng C,Liu G

Mon Jan 20 00:00:00 EST 2020

Crocin attenuates isoprenaline-induced myocardial fibrosis by targeting TLR4/NF-κB signaling: connecting oxidative stress, inflammation, and apoptosis.

Naunyn-Schmiedeberg's archives of pharmacology

Jin W,Zhang Y,Xue Y,Han X,Zhang X,Ma Z,Sun S,Chu X,Cheng J,Guan S,Li Z,Chu L

Wed Jan 01 00:00:00 EST 2020

Non-invasive detection of local tissue responses to predict pressure ulcer development in mouse models.

Journal of tissue viability

Kimura N,Nakagami G,Minematsu T,Sanada H

Sat Feb 01 00:00:00 EST 2020

Ameliorative effects and mechanism of crocetin in arsenic trioxide‑induced cardiotoxicity in rats.

Molecular medicine reports

Zhao Z,Li J,Zheng B,Liang Y,Shi J,Zhang J,Han X,Chu L,Chu X,Gao Y

Tue Dec 01 00:00:00 EST 2020

TLR4 gene in the regulation of periodontitis and its molecular mechanism.

Experimental and therapeutic medicine

Qi W,Yang X,Ye N,Li S,Han Q,Huang J,Wu B

Sun Sep 01 00:00:00 EDT 2019

Telbivudine antagonizes TLR4 to inhibit the epithelial-to-mesenchymal transition in human proximal tubular epithelial cells in vitro.

International immunopharmacology

Chen J,Li D,Luo E

Sun Sep 01 00:00:00 EDT 2019

Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA 4-Dependent CCN 1 Secretion.

Journal of the American Heart Association

Cui S,Xue L,Yang F,Dai S,Han Z,Liu K,Liu B,Yuan Q,Cui Z,Zhang Y,Xu F,Chen Y

Tue Sep 18 00:00:00 EDT 2018

Effects of Ketamine on Levels of Inflammatory Cytokines IL-6, IL-1β, and TNF-α in the Hippocampus of Mice Following Acute or Chronic Administration.

Frontiers in pharmacology

Li Y,Shen R,Wen G,Ding R,Du A,Zhou J,Dong Z,Ren X,Yao H,Zhao R,Zhang G,Lu Y,Wu X

Fri Mar 11 00:00:00 EST 2022

Effects of Ketamine on Levels of Inflammatory Cytokines IL-6, IL-1β, and TNF-α in the Hippocampus of Mice Following Acute or Chronic Administration.

Frontiers in pharmacology

Li Y,Shen R,Wen G,Ding R,Du A,Zhou J,Dong Z,Ren X,Yao H,Zhao R,Zhang G,Lu Y,Wu X

Fri Mar 11 00:00:00 EST 2022

Bioinformatics

Protein Aliases: CTLA8; Hematopoietin-1; IL 1 a; IL 1α; IL-1 alpha; IL17; IL1α; ILN; Interleukin; interleukin 1, alpha; interleukin 17; Interleukin-1 alpha; Interleukin1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha; RP23-160G19.8

View more View less

Gene Aliases: IL-1A; IL1; IL1-ALPHA; IL1A; IL1F1

View more View less

UniProt ID: (Human) P01583, (Mouse) P01582

View more View less

Entrez Gene ID: (Human) 3552, (Mouse) 16175

View more View less

Function(s)

cytokine activity interleukin-1 receptor binding copper ion binding protein binding interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL 1 alpha Polyclonal Antibody

IL 1 alpha Antibody (16765-1-AP) in ICC/IF

Product Details

16765-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!