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IL-1 alpha Antibody (500-P180GBT-25UG) in WB
Western Blot: To detect Rat IL-1alpha by Western Blot analysis IL-1 alpha Polyclonal Antibody, Biotin (Product # 500-P180GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P180GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Rat IL-1alpha
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929679
Product Specific Information
AA Sequence of recombinant protein: MAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDSKYPV TLKVSNTQLF VSAQGEDKPV LLKEIPETPK LITGSETDLI FFWEKINSKN YFTSAAFPEL LIATKEQSQV HLARGLPSMI DFQIS.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Rat IL-1alpha. Anti-Rat IL-1alpha-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Rat IL-1alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-1alpha (500-P180G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-1alpha.
Western Blot: To detect Rat IL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P180GBT-1MG will be provided as 2 x 500 µg
Target Information
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTLA8; IL 1 a; IL 1α; IL-1 alpha; IL17; IL1α; ILN; Interleukin; interleukin 17; Interleukin-1 alpha; Interleukin1 alpha; RP23-160G19.8
Gene Aliases: IL-1 alpha; Il1a
UniProt ID: (Rat) P16598
Entrez Gene ID: (Rat) 24493
fever generation
response to hypoxia
connective tissue replacement involved in inflammatory response wound healing
acute inflammatory response
inflammatory response
immune response
spermatogenesis
heart development
negative regulation of cell proliferation
response to heat
response to organic substance
response to ozone
response to organonitrogen compound
response to gamma radiation
positive regulation of vascular endothelial growth factor production
positive regulation of steroid biosynthetic process
response to organic cyclic compound
cytokine-mediated signaling pathway
keratinization
positive regulation of prostaglandin secretion
response to lipopolysaccharide
positive regulation of interleukin-6 production
positive regulation of stress-activated MAPK cascade
response to L-ascorbic acid
cellular response to heat
ectopic germ cell programmed cell death
wound healing
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of interleukin-2 biosynthetic process
positive regulation of angiogenesis
positive regulation of mitotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
response to copper ion
positive regulation of cytokine secretion
positive regulation of cell division
regulation of sensory perception of pain
positive regulation of ERK1 and ERK2 cascade
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of neutrophil migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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