Invitrogen

IL-1 alpha Polyclonal Antibody, Biotin, PeproTech®

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Datasheet
Protocols
Questions & Answers

Product Details

500-P180GBT-25UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
-
Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Rat IL-1alpha
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929679

Product Specific Information

AA Sequence of recombinant protein: MAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDSKYPV TLKVSNTQLF VSAQGEDKPV LLKEIPETPK LITGSETDLI FFWEKINSKN YFTSAAFPEL LIATKEQSQV HLARGLPSMI DFQIS.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Rat IL-1alpha. Anti-Rat IL-1alpha-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Rat IL-1alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-1alpha (500-P180G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-1alpha.

Western Blot: To detect Rat IL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P180GBT-1MG will be provided as 2 x 500 µg

Target Information

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CTLA8; IL 1 a; IL 1α; IL-1 alpha; IL17; IL1α; ILN; Interleukin; interleukin 17; Interleukin-1 alpha; Interleukin1 alpha; RP23-160G19.8

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Gene Aliases: IL-1 alpha; Il1a

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UniProt ID: (Rat) P16598

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Entrez Gene ID: (Rat) 24493

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Function(s)
cytokine activity interleukin-1 receptor binding copper ion binding interleukin superfamily
Process(es)
fever generation response to hypoxia connective tissue replacement involved in inflammatory response wound healing acute inflammatory response inflammatory response immune response spermatogenesis heart development negative regulation of cell proliferation response to heat response to organic substance response to ozone response to organonitrogen compound response to gamma radiation positive regulation of vascular endothelial growth factor production positive regulation of steroid biosynthetic process response to organic cyclic compound cytokine-mediated signaling pathway keratinization positive regulation of prostaglandin secretion response to lipopolysaccharide positive regulation of interleukin-6 production positive regulation of stress-activated MAPK cascade response to L-ascorbic acid cellular response to heat ectopic germ cell programmed cell death wound healing positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of interleukin-2 biosynthetic process positive regulation of angiogenesis positive regulation of mitotic nuclear division positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade response to copper ion positive regulation of cytokine secretion positive regulation of cell division regulation of sensory perception of pain positive regulation of ERK1 and ERK2 cascade extrinsic apoptotic signaling pathway in absence of ligand positive regulation of neutrophil migration
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