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Invitrogen
IL-1 alpha Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
IL-1 alpha Antibody (PA5-47876) in Neu
Neutralization of IL-1 alpha in D10.G4.1 mouse helper T cell line. Samples were incubated in IL-1 alpha polyclonal antibody (Product # PA5-47876) using a dilution of 75 pg/mL. Recombinant Porcine IL‚1α/IL‚1F1 stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‚1α/IL‚1F1 is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‚1α/IL‚1F1 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.006-0.0... View More
Product Details
PA5-47876
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Pig
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant porcine IL-1 alpha Gln119-Ser270
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609300
Product Specific Information
In direct ELISAs, approximately 20% cross-reactivity with recombinant human IL-1 alpha is observed and 10% cross-reactivity with recombinant mouse IL-1 alpha and recombinant rat IL-1 alpha is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTLA8; IL 1 a; IL 1α; IL-1 alpha; IL17; IL1α; ILN; Interleukin; interleukin 17; Interleukin-1 alpha; Interleukin1 alpha; RP23-160G19.8
Gene Aliases: IL-1alpha; IL1A
UniProt ID: (Pig) P18430
Entrez Gene ID: (Pig) 397094
fever generation
connective tissue replacement involved in inflammatory response wound healing
immune response
negative regulation of cell proliferation
positive regulation of vascular endothelial growth factor production
cellular response to heat
ectopic germ cell programmed cell death
positive regulation of angiogenesis
positive regulation of mitotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
response to copper ion
positive regulation of cytokine secretion
positive regulation of cell division
extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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