Invitrogen

IL-1 beta Polyclonal Antibody, Biotin, eBioscience™

FIGURE: 1 / 7

IL-1 beta Antibody (13-7112-81) in ELISA

The Journal of experimental medicine 2020 - Figure 2. NLRC4 phosphorylation is neither sufficient nor strictly required for response to cytosolic flagellin. (A-E) Macrophages were either left unprimed (A and B) or primed 4 h with 1 ug/ml Pam3CSK4 (C-E) and then treated with 4 ug/ml PA and the indicated dose of LFn-FlaA to activate NLRC4. LDH release (A and C), PI uptake (B and D), or IL-1beta release (E) was measured after 4 h. All data are represent... View More

IL-1 beta Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

13-7112-81

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

ELISA (ELISA)

1-6 µg/mL

View 20 publications 20 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466924

Product Specific Information

Description: The biotinylated polyclonal rabbit antibody reacts with mouse interleukin-1beta (IL-1beta), a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.

Applications Reported: Biotin anti-mouse interleukin-1 beta (IL-1 beta) has been reported for use as the detection antibody for a mouse IL-1 beta ELISA.

Applications Tested: The biotinylated polyclonal rabbit anti-mouse IL-1 beta antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-1 beta in combination with the unlabelled B122 (14-7012) antibody for capture and recombinant mouse IL-1 beta (14-8012) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 1.0 - 6.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A flagellin-conjugate protein induces dual NLRC4- and NLRP3-inflammasome activation which modulates inflammatory cytokine secretion from macrophages.

Frontiers in immunology

Lin YJ,Jamin A,Wolfheimer S,Fiedler A,Junker AC,Goretzki A,Scheurer S,Schülke S

13-7112-81 was used in ELISA to investigate the mechanisms underlying the production of IL-1β from rFlaA:Betv1 stimulated macrophages.

Mon Apr 10 00:00:00 EDT 2023

Histological changes of cervical tumours following Zanthoxylum acanthopodium DC treatment, and its impact on cytokine expression.

Saudi journal of biological sciences

Simanullang RH,Situmorang PC,Herlina M,Silalahi B,Manurung SS

13-7112-81 was used in ELISA to conclude that ZAM may be a potential target for molecular cytokine therapy for cervical cancer.

Fri Apr 01 00:00:00 EDT 2022

Histological changes of cervical tumours following Zanthoxylum acanthopodium DC treatment, and its impact on cytokine expression.

Saudi journal of biological sciences

Simanullang RH,Situmorang PC,Herlina M,Silalahi B,Manurung SS

13-7112-81 was used in ELISA to conclude that ZAM may be a potential target for molecular cytokine therapy for cervical cancer.

Fri Apr 01 00:00:00 EDT 2022

The Fusion Protein rFlaA:Betv1 Modulates DC Responses by a p38-MAPK and COX2-Dependent Secretion of PGE₂ from Epithelial Cells.

Cells

Lin YJ,Flaczyk A,Wolfheimer S,Goretzki A,Jamin A,Wangorsch A,Vieths S,Scheurer S,Schülke S

13-7112-81 was used in Enzyme-linked immunosorbent assay to investigate the immune-modulatory effects of rFlaA:Betv1 on airway epithelial cells. LA-4 mouse lung epithelial cells were stimulated with rFlaA:Betv1 in the presence/absence of various inhibitors with cytokine- and chemokine secretion quantified by ELISA and activation of intracellular signaling cascades demonstrated by Western blot (WB).

Sat Dec 04 00:00:00 EST 2021

Origin and Function of Stress-Induced IL-6 in Murine Models.

Cell

Qing H,Desrouleaux R,Israni-Winger K,Mineur YS,Fogelman N,Zhang C,Rashed S,Palm NW,Sinha R,Picciotto MR,Perry RJ,Wang A

13-7112-81 was used in ELISA to study the cytokine interleukin-6 (IL-6) and its induction following acute stress in mouse models.

Thu Jul 23 00:00:00 EDT 2020

NLRC4 inflammasome activation is NLRP3- and phosphorylation-independent during infection and does not protect from melanoma.

The Journal of experimental medicine

Tenthorey JL,Chavez RA,Thompson TW,Deets KA,Vance RE,Rauch I

13-7112 was used in an ELISA assay to investigate the mechanism and biological functions of NAIP/NLRC4 activation.

Mon Jul 06 00:00:00 EDT 2020

NLRC4 inflammasome activation is NLRP3- and phosphorylation-independent during infection and does not protect from melanoma.

The Journal of experimental medicine

Tenthorey JL,Chavez RA,Thompson TW,Deets KA,Vance RE,Rauch I

13-7112 was used in an ELISA assay to investigate the mechanism and biological functions of NAIP/NLRC4 activation.

Mon Jul 06 00:00:00 EDT 2020

Thioredoxin-1 distinctly promotes NF-κB target DNA binding and NLRP3 inflammasome activation independently of Txnip.

eLife

Muri J,Thut H,Feng Q,Kopf M

Published figure using IL-1 beta polyclonal antibody (Product # 13-7112-81) in ELISA

Tue Feb 25 00:00:00 EST 2020

Electrophilic Nrf2 activators and itaconate inhibit inflammation at low dose and promote IL-1β production and inflammatory apoptosis at high dose.

Redox biology

Muri J,Wolleb H,Broz P,Carreira EM,Kopf M

13-7112 was used in an ELISA assay to find that exposure of bone marrow-derived dendritic cells (BMDCs) to low concentrations of a variety of electrophilic Nrf2 activators including itaconate prior to Toll-like receptor (TLR) stimulation inhibits transcription of pro-inflammatory cytokines (such as interleukin [IL]-12 and IL-1β) by activation of Nrf2.

Tue Sep 01 00:00:00 EDT 2020

Identification of a herpes simplex virus 1 gene encoding neurovirulence factor by chemical proteomics.

Nature communications

Kato A,Adachi S,Kawano S,Takeshima K,Watanabe M,Kitazume S,Sato R,Kusano H,Koyanagi N,Maruzuru Y,Arii J,Hatta T,Natsume T,Kawaguchi Y

13-7112 was used in an ELISA assay to identify nine previously cryptic orphan protein coding sequences whose translated products are expressed in HSV-1-infected cells.

Tue Sep 29 00:00:00 EDT 2020

Cyclopentenone Prostaglandins and Structurally Related Oxidized Lipid Species Instigate and Share Distinct Pro- and Anti-inflammatory Pathways.

Cell reports

Muri J,Feng Q,Wolleb H,Shamshiev A,Ebner C,Tortola L,Broz P,Carreira EM,Kopf M

13-7112 was used in an ELISA assay to identify that cyclopentenone-containing prostaglandins such as 15d-PGJ2 and structurally related oxidized phospholipid species possess a dual and opposing bioactivity in inflammation, depending on their concentration.

Tue Mar 31 00:00:00 EDT 2020

Peli1 controls the survival of dopaminergic neurons through modulating microglia-mediated neuroinflammation.

Scientific reports

Dai D,Yuan J,Wang Y,Xu J,Mao C,Xiao Y

Published figure using IL-1 beta polyclonal antibody (Product # 13-7112-81) in ELISA

Wed May 29 00:00:00 EDT 2019

Outer Membrane Protein of Gut Commensal Microorganism Induces Autoantibody Production and Extra-Intestinal Gland Inflammation in Mice.

International journal of molecular sciences

Yanagisawa N,Ueshiba H,Abe Y,Kato H,Higuchi T,Yagi J

Published figure using IL-1 beta polyclonal antibody (Product # 13-7112-81) in ELISA

Fri Oct 19 00:00:00 EDT 2018

Pyroptosis Induction and Detection.

Current protocols in immunology

den Hartigh AB,Fink SL

13-7112 was used in an ELISA assay to assess loss of plasma membrane integrity following pyroptosis.

Wed Aug 01 00:00:00 EDT 2018

Dnase1L3 Regulates Inflammasome-Dependent Cytokine Secretion.

Frontiers in immunology

Shi G,Abbott KN,Wu W,Salter RD,Keyel PA

13-7112 was used in an ELISA assay to investigate the possibility that immune activation, specifically inflammasome activation, is regulated by Dnase1L3.

Fri Aug 04 00:00:00 EDT 2023

Orally administered sodium 4-phenylbutyrate suppresses the development of dextran sulfate sodium-induced colitis in mice.

Experimental and therapeutic medicine

Ono K,Nimura S,Hideshima Y,Nabeshima K,Nakashima M

13-7112 was used in an ELISA assay to investigate the effects of orally administered sodium 4-phenylbutyrate in mice.

Fri Dec 01 00:00:00 EST 2017

Cyclo(Phe-Pro) produced by the human pathogen Vibrio vulnificus inhibits host innate immune responses through the NF-κB pathway.

Infection and immunity

Kim K,Kim NJ,Kim SY,Kim IH,Kim KS,Lee GR

13-7112 was used in an ELISA assay to investigate the effects of a secondary metabolite produced by the lethal human bacterial pathogen Vibrio vulnificus on the mammalian innate immune system.

Sun Mar 01 00:00:00 EST 2015

NLRC4 expression in intestinal epithelial cells mediates protection against an enteric pathogen.

Mucosal immunology

Nordlander S,Pott J,Maloy KJ

13-7112 was used in an ELISA assay to study how early NLRC4 sensing in intestinal epithelial cells regulates colonization by an extracellular bacterial pathogen and limits subsequent intestinal damage.

Tue Jul 01 00:00:00 EDT 2014

Nlrp3 activation in the intestinal epithelium protects against a mucosal pathogen.

Mucosal immunology

Song-Zhao GX,Srinivasan N,Pott J,Baban D,Frankel G,Maloy KJ

13-7112 was used in an ELISA assay to investigate how protection from disease is mediated through Nlrp3 activation in nonhematopoietic cells and is initiated very early after infection.

Tue Jul 01 00:00:00 EDT 2014

Nrf2 is essential for cholesterol crystal-induced inflammasome activation and exacerbation of atherosclerosis.

European journal of immunology

Freigang S,Ampenberger F,Spohn G,Heer S,Shamshiev AT,Kisielow J,Hersberger M,Yamamoto M,Bachmann MF,Kopf M

13-7112 was used in an ELISA assay to identify NF-E2-related 2 as an essential positive regulator of inflammasome activation and IL-1-mediated vascular inflammation.

Fri Jul 01 00:00:00 EDT 2011

Tim3 binding to galectin-9 stimulates antimicrobial immunity.

The Journal of experimental medicine

Jayaraman P,Sada-Ovalle I,Beladi S,Anderson AC,Dardalhon V,Hotta C,Kuchroo VK,Behar SM

13-7112 was used in an ELISA assay to explore the role of the Th1 cell surface molecule Tim3 in the regulation of antimicrobial immunity.

Mon Oct 25 00:00:00 EDT 2010

IL-18 and IL-33 elicit Th2 cytokines from basophils via a MyD88- and p38alpha-dependent pathway.

Journal of leukocyte biology

Kroeger KM,Sullivan BM,Locksley RM

Published figure using IL-1 beta polyclonal antibody (Product # 13-7112-81) in ELISA

Thu Oct 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta

View more View less

Gene Aliases: Il-1b; IL-1beta; Il1b

View more View less

UniProt ID: (Mouse) P10749

View more View less

Entrez Gene ID: (Mouse) 16176

View more View less

Function(s)

receptor binding cytokine activity interleukin-1 receptor binding protein domain specific binding interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-1 beta Polyclonal Antibody, Biotin, eBioscience™

IL-1 beta Antibody (13-7112-81) in ELISA

Product Details

13-7112-81

Immunohistochemistry (IHC)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!