Invitrogen

IL-1 beta Monoclonal Antibody (ILB1-H67)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

15 Published Figures

18 References

View all (119) IL-1 beta antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 19

IL-1 beta Antibody (M421B) in WB

Western blot was performed using Anti-IL-1 beta Monoclonal Antibody (ILB1-H67) (Product # M421B) and a 35kDa band corresponding to Interleukin-1 beta was observed across in THP-1, upon treatment with Lipopolysaccharide. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 (Lipopolysaccharide (1 µg/mL), 18 hours) (Lane 2), RAW 264.7 (Lane 3), BeWo (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBl... View More

Product Image Gallery

Product Details

M421B

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

ILB1-H67

Immunogen

Recombinant human IL-1 beta

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223567

Product Specific Information

M421B targets IL-1 beta in ELISA, and WB applications and shows reactivity with Human samples.

A precipitate may be present in the solution. Mix for at least five seconds prior to use.

M421B detects IL-1 beta which has a predicted molecular weight of approximately 31 kDa.

The M421B IL1b antibody (clone ILB1-H67) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M420BB (biotinylated conjugate of clone ILB1-H6). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody M421B (clone ILB1-H67) and biotinylated antibody M420BB (clone ILB1-H6) have successfully been used in combination with recombinant IL1 beta protein SIL1B in ELISA applications.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Noise exposure and its relationship with postinfarction cardiac remodeling: implications for NLRP3 inflammasome activation.

Bioengineered

Wei Y,Li W,Yang S,Zhong P,Bi Y,Tang Y

M421B was used in Western Blotting to reveal a previously unidentified association between NLRP3 inflammasome activation and noise exposure, underscoring the significance of effective noise prevention in improving postinfarction prognosis.

Sun May 01 00:00:00 EDT 2022

Long non-coding RNA MEG3 promotes tumor necrosis factor-alpha induced oxidative stress and apoptosis in interstitial cells of cajal via targeting the microRNA-21 /I-kappa-B-kinase beta axis.

Bioengineered

Li J,Bai J,Tuerdi N,Liu K

M421B was used in Western Blotting to conclude that MEG3 mediates inflammation, oxidative stress and apoptosis in TNF-α-treated ICC via the miR-21/IKKB-NF-κB axis.

Fri Apr 01 00:00:00 EDT 2022

Intermittent fasting therapy promotes insulin sensitivity by inhibiting NLRP3 inflammasome in rat model.

Annals of palliative medicine

Liang BJ,Liao SR,Huang WX,Huang C,Liu HS,Shen WZ

M421B was used in Western Blotting to suggest that FT can ameliorate the high-fat diet-and STZ-induced IR in rats through inhibition of NLRP3 inflammasome.

Sat May 01 00:00:00 EDT 2021

HSP70-Mediated NLRP3 Inflammasome Suppression Underlies Reversal of Acute Kidney Injury Following Extracellular Vesicle and Focused Ultrasound Combination Therapy.

International journal of molecular sciences

Ullah M,Liu DD,Rai S,Concepcion W,Thakor AS

M421B was used in Western Blotting to demonstrates the ability of pulsed focused ultrasound to enhance extracellular vesicles therapy for AKI.

Mon Jun 08 00:00:00 EDT 2020

HSP70-Mediated NLRP3 Inflammasome Suppression Underlies Reversal of Acute Kidney Injury Following Extracellular Vesicle and Focused Ultrasound Combination Therapy.

International journal of molecular sciences

Ullah M,Liu DD,Rai S,Concepcion W,Thakor AS

M421B was used in Western Blotting to demonstrates the ability of pulsed focused ultrasound to enhance extracellular vesicles therapy for AKI.

Mon Jun 08 00:00:00 EDT 2020

Neutralization of interleukin-17A alleviates burn-induced intestinal barrier disruption via reducing pro-inflammatory cytokines in a mouse model.

Burns & trauma

Song Y,Li Y,Xiao Y,Hu W,Wang X,Wang P,Zhang X,Yang J,Huang Y,He W,Huang C

Published figure using IL-1 beta monoclonal antibody (Product # M421B) in Western Blot

Tue Apr 12 00:00:00 EDT 2022

Borrelia burgdorferi infection regulates CD1 expression in human cells and tissues via IL1-β.

European journal of immunology

Yakimchuk K,Roura-Mir C,Magalhaes KG,de Jong A,Kasmar AG,Granter SR,Budd R,Steere A,Pena-Cruz V,Kirschning C,Cheng TY,Moody DB

Published figure using IL-1 beta monoclonal antibody (Product # M421B) in ELISA

Tue Mar 01 00:00:00 EST 2011

Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease.

Neurobiology of aging

Loewenbrueck KF,Tigno-Aranjuez JT,Boehm BO,Lehmann PV,Tary-Lehmann M

M421B was used in ELISA to study the role of Th1 responses to beta amyloid in Down syndrome and Alzheimer disease

Fri Oct 01 00:00:00 EDT 2010

Relationship between antipyretic effects and cytokine levels in uncomplicated falciparum malaria during different treatment regimes.

Acta tropica

Hugosson E,Montgomery SM,Premji Z,Troye-Blomberg M,Björkman A

M421B was used in ELISA to investigate the effects of antipyretics on cytokine levels in uncomplicated falciparum malaria

Tue Aug 01 00:00:00 EDT 2006

Protein microarray platform for the multiplex analysis of biomarkers in human sera.

Journal of immunological methods

Urbanowska T,Mangialaio S,Zickler C,Cheevapruk S,Hasler P,Regenass S,Legay F

M421B was used in ELISA to develop a protein microarray platform for the multiplex analysis of biomarkers in human sera

Fri Oct 20 00:00:00 EDT 2006

Immunologic testing of xeno-derived osteochondral grafts using peripheral blood mononuclear cells from healthy human donors.

BMC musculoskeletal disorders

Hetherington VJ,Kawalec JS,Dockery DS,Targoni OS,Lehmann PV,Nadler D

Published figure using IL-1 beta monoclonal antibody (Product # M421B) in ELISA

Wed Jun 29 00:00:00 EDT 2005

Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.

Rheumatology (Oxford, England)

Schotte H,Schlüter B,Willeke P,Mickholz E,Schorat MA,Domschke W,Gaubitz M

M421B was used in ELISA to study the effect of etanercept treatment on proinflammatory cytokine-secreting peripheral blood mononuclear cells in rheumatoid arthritic patients

Sun Aug 01 00:00:00 EDT 2004

Differing caspase-1 activation states in monocyte versus macrophage models of IL-1beta processing and release.

Journal of leukocyte biology

Kahlenberg JM,Dubyak GR

Published figure using IL-1 beta monoclonal antibody (Product # M421B) in ELISA

Wed Sep 01 00:00:00 EDT 2004

HIV-1 coat protein gp120 stimulates interleukin-1beta secretion from human neuroblastoma cells: evidence for a role in the mechanism of cell death.

British journal of pharmacology

Corasaniti MT,Bilotta A,Strongoli MC,Navarra M,Bagetta G,Di Renzo G

M421B was used in ELISA to investigate the role of interleukin 1 beta during the cell death induced by HIV infection

Thu Nov 01 00:00:00 EST 2001

Immune phenotype and intracellular cytokine production of peripheral blood mononuclear cells from postmenopausal patients with osteoporotic fractures.

Experimental gerontology

Pietschmann P,Grisar J,Thien R,Willheim M,Kerschan-Schindl K,Preisinger E,Peterlik M

M421B was used in immunocytochemistry to analyze peripheral blood mononuclear cell surface markers and intracellular cytokine production in postmenopausal osteoporosis patients

Thu Nov 01 00:00:00 EST 2001

Intrahepatic expression of interleukin-1beta and tumor necrosis factor-alpha in chronic hepatitis C.

The Journal of infectious diseases

Dumoulin FL,Leifeld L,Honecker U,Sauerbruch T,Spengler U

M421B was used in immunohistochemistry to investigate the intrahepatic expression of interleukin 1 beta and tumor necrosis factor alpha in liver specimens with chronic hepatitis C

Mon Nov 01 00:00:00 EST 1999

Prostaglandin E2 induces the final maturation of IL-12-deficient CD1a+CD83+ dendritic cells: the levels of IL-12 are determined during the final dendritic cell maturation and are resistant to further modulation.

Journal of immunology (Baltimore, Md. : 1950)

Kaliński P,Schuitemaker JH,Hilkens CM,Kapsenberg ML

M421B was used in ELISA to investigate the roles of prostaglandin E2 and interleukin 10 in CD1a+ dendritic cell maturation

Tue Sep 15 00:00:00 EDT 1998

Regulation of interleukin-12 by complement receptor 3 signaling.

The Journal of experimental medicine

Marth T,Kelsall BL

M421B was used in ELISA to assess the effect of complement receptor 3 on interleukin 12 production

Mon Jun 02 00:00:00 EDT 1997

IL-12-deficient dendritic cells, generated in the presence of prostaglandin E2, promote type 2 cytokine production in maturing human naive T helper cells.

Journal of immunology (Baltimore, Md. : 1950)

Kaliński P,Hilkens CM,Snijders A,Snijdewint FG,Kapsenberg ML

M421B was used in ELISA to investigate the importance of PGE2 during dendritic cell development

Tue Jul 01 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: Catabolin; Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; interleukin 1, beta; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); preinterleukin 1 beta; Pro interleukin 1 beta; pro-interleukin-1-beta

View more View less

Gene Aliases: IL-1; Il-1b; IL-1beta; IL1-BETA; IL1B; IL1F2

View more View less

UniProt ID: (Human) P01584, (Mouse) P10749

View more View less

Entrez Gene ID: (Human) 3553, (Mouse) 16176

View more View less

Function(s)

cytokine activity interleukin-1 receptor binding protein domain specific binding receptor binding interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-1 beta Monoclonal Antibody (ILB1-H67)

IL-1 beta Antibody (M421B) in WB

Product Details

M421B

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!