Invitrogen

IL-10 Monoclonal Antibody, PeproTech®

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Datasheet
Protocols
Questions & Answers

Product Details

500-M86-500UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.25-0.50 µg/mL
-

ELISA (ELISA)

8.0-10.0 µg/mL
-

In vitro Assay (IV)

-
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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human IL-10
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_3074170

Product Specific Information

AA Sequence of recombinant protein: MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N.

Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human IL-10. Anti-Human IL-10-specific antibody was purified from tissue culture supernatant by Protein A affinity chromatography.

Sandwich ELISA: Assuming 100 µL/well, a concentration of 8.0-10.0 µg/mL of this antibody will detect at least 2000 pg/mL of Recombinant Human IL-10 when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human IL-10 (500-P20BT) as the detection antibody at a concentration of approximately 0.05- 0.10 µg/mL.

Western Blot: To detect Human IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-10 is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

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Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

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UniProt ID: (Human) P22301

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Entrez Gene ID: (Human) 3586

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Function(s)
cytokine activity interleukin-10 receptor binding protein binding growth factor activity
Process(es)
response to molecule of bacterial origin negative regulation of cytokine secretion involved in immune response negative regulation of chronic inflammatory response to antigenic stimulus positive regulation of B cell apoptotic process inflammatory response cytoplasmic sequestering of NF-kappaB cell-cell signaling aging regulation of gene expression response to activity response to inactivity hemopoiesis B cell differentiation leukocyte chemotaxis negative regulation of myeloid dendritic cell activation negative regulation of B cell proliferation negative regulation of interferon-gamma production negative regulation of interleukin-1 production negative regulation of interleukin-12 production negative regulation of interleukin-18 production negative regulation of interleukin-6 production negative regulation of interleukin-8 production negative regulation of tumor necrosis factor production receptor biosynthetic process response to insulin response to carbon monoxide cellular response to hepatocyte growth factor stimulus type 2 immune response B cell proliferation negative regulation of T cell proliferation response to drug negative regulation of tumor necrosis factor biosynthetic process defense response to bacterium defense response to protozoan negative regulation of apoptotic process negative regulation of growth of symbiont in host negative regulation of nitric oxide biosynthetic process regulation of isotype switching negative regulation of MHC class II biosynthetic process positive regulation of MHC class II biosynthetic process negative regulation of interferon-alpha biosynthetic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade positive regulation of cytokine secretion negative regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity response to glucocorticoid regulation of sensory perception of pain negative regulation of cytokine activity branching involved in labyrinthine layer morphogenesis cellular response to lipopolysaccharide cellular response to estradiol stimulus negative regulation of chemokine (C-C motif) ligand 5 production regulation of complement-dependent cytotoxicity negative regulation of endothelial cell apoptotic process
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