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IL-10 Antibody (500-P139-1MG) in ELISA
Sandwich ELISA: To detect Rat IL-10 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IL-10 Polyclonal Antibody (Product # 500-P139-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL-10 Polyclonal Antibody, Biotin (Product # 500-P139BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-10.
Product Details
500-P139-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Rat IL-10
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929439
Product Specific Information
AA Sequence of recombinant protein: SKGHSIKGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNIVLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-10. Anti-Rat IL-10-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-10 matrix.
Sandwich ELISA: To detect Rat IL-10 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-10 (500-P139Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-10.
Western Blot: To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1
Gene Aliases: Il-10; Il10; IL10X
UniProt ID: (Rat) P29456
Entrez Gene ID: (Rat) 25325
negative regulation of cytokine production
response to molecule of bacterial origin
negative regulation of cytokine secretion involved in immune response
negative regulation of chronic inflammatory response to antigenic stimulus
positive regulation of B cell apoptotic process
inflammatory response
immune response
aging
response to organic substance
regulation of gene expression
response to activity
response to inactivity
negative regulation of myeloid dendritic cell activation
negative regulation of B cell proliferation
response to lipopolysaccharide
negative regulation of interferon-gamma production
negative regulation of interleukin-12 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
receptor biosynthetic process
response to insulin
response to carbon monoxide
response to drug
negative regulation of tumor necrosis factor biosynthetic process
defense response to bacterium
negative regulation of apoptotic process
negative regulation of growth of symbiont in host
negative regulation of nitric oxide biosynthetic process
positive regulation of MHC class II biosynthetic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
positive regulation of cytokine secretion
negative regulation of inflammatory response
negative regulation of membrane protein ectodomain proteolysis
positive regulation of sequence-specific DNA binding transcription factor activity
response to glucocorticoid
regulation of sensory perception of pain
branching involved in labyrinthine layer morphogenesis
cellular response to lipopolysaccharide
cellular response to estradiol stimulus
regulation of complement-dependent cytotoxicity
negative regulation of endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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