Invitrogen

IL-10 Monoclonal Antibody (2A5)

1 Published Figure

23 References

View all (98) IL-10 antibodies

Datasheet

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Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (1)

FIGURE: 1 / 1

IL-10 Antibody (MM010) in Neu

Nature medicine 2009 - Published figure using IL-10 monoclonal antibody (Product # MM010) in Neutralization

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Product Details

MM010

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

View 13 publications 13 publications

Neutralization (Neu)

View 10 publications 10 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

2A5

Immunogen

Recombinant mouse IL-10

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223521

Product Specific Information

MM010 targets IL-10 in WB applications and shows reactivity with mouse samples.

The MM010 immunogen is recombinant mouse IL-10.

MM010 detects IL-10 which has a predicted molecular weight of approximately 19 kDa.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hypoxia-inducible factor 2alpha regulates macrophage function in mouse models of acute and tumor inflammation.

The Journal of clinical investigation

Imtiyaz HZ,Williams EP,Hickey MM,Patel SA,Durham AC,Yuan LJ,Hammond R,Gimotty PA,Keith B,Simon MC

MM010 was used in blocking or activating experiment to investigate the effect of hypoxia-inducible factor 2alpha in innate immunity

Sun Aug 01 00:00:00 EDT 2010

Bone marrow stromal cells use TGF-beta to suppress allergic responses in a mouse model of ragweed-induced asthma.

Proceedings of the National Academy of Sciences of the United States of America

Nemeth K,Keane-Myers A,Brown JM,Metcalfe DD,Gorham JD,Bundoc VG,Hodges MG,Jelinek I,Madala S,Karpati S,Mezey E

MM010 was used in ELISA to study the effect of TGF-beta on allergic responses

Tue Mar 23 00:00:00 EDT 2010

Bone marrow stromal cells attenuate sepsis via prostaglandin E(2)-dependent reprogramming of host macrophages to increase their interleukin-10 production.

Nature medicine

Németh K,Leelahavanichkul A,Yuen PS,Mayer B,Parmelee A,Doi K,Robey PG,Leelahavanichkul K,Koller BH,Brown JM,Hu X,Jelinek I,Star RA,Mezey E

Published figure using IL-10 monoclonal antibody (Product # MM010) in Neutralization

Thu Jan 01 00:00:00 EST 2009

Augmented expression of tumour necrosis factor-alpha induced by lipopolysaccharide in spleen of human monocyte chemoattractant protein-1 transgenic mouse enhances the lipopolysaccharide sensitivity of the marginal zone macrophages.

Immunology

Ato M,Iwabuchi K,Shimada S,Mukaida N,Onoé K

MM010 was used in blocking/activating experiment to investigate the phenotype of hMCP-1 transgenic mouse stimulated by lipopolysaccharide

Thu Aug 01 00:00:00 EDT 2002

Increased survival in sepsis by in vivo adenovirus-induced expression of IL-10 in dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Oberholzer A,Oberholzer C,Bahjat KS,Ungaro R,Tannahill CL,Murday M,Bahjat FR,Abouhamze Z,Tsai V,LaFace D,Hutchins B,Moldawer LL,Clare-Salzler MJ

MM010 was used in ELISA to study the effect of interleukin 10 on the survival from septic shock

Mon Apr 01 00:00:00 EST 2002

The Trypanosoma cruzi trans-sialidase is a T cell-independent B cell mitogen and an inducer of non-specific Ig secretion.

International immunology

Gao W,Wortis HH,Pereira MA

MM010 was used in ELISA to study the role of Trypanosoma cruzi trans-sialidase in B cell proliferation and Ig secretion

Fri Mar 01 00:00:00 EST 2002

Suppressor T cells regulate the nonanergic cell population that remains after peripheral tolerance is induced to the Mls-1 antigen in T cell receptor Vbeta 8.1 transgenic mice.

Proceedings of the National Academy of Sciences of the United States of America

Maeda H,Fujimoto S,Greene MI

MM010 was used in blocking/activating experiment to investigate the roles of suppressor T cells

Tue Nov 21 00:00:00 EST 2000

Immunogenicity and arthritogenicity of recombinant CB10 in B10.RIII mice.

Journal of immunology (Baltimore, Md. : 1950)

Tang B,Brand DD,Chiang TM,Stuart JM,Kang AH,Myers LK

MM010 was used in ELISA to investigate the role of CD10 in immunogenicity and arthritogenicity

Sat Jan 01 00:00:00 EST 2000

Oral administration of chitin down-regulates serum IgE levels and lung eosinophilia in the allergic mouse.

Journal of immunology (Baltimore, Md. : 1950)

Shibata Y,Foster LA,Bradfield JF,Myrvik QN

MM010 was used in ELISA to investigate the effect of chitin administration on serum IgE levels and lung eosinophilia

Tue Feb 01 00:00:00 EST 2000

Interleukin-10 has different effects on proliferation of Listeria monocytogenes in livers and spleens of mice.

Infection and immunity

Samsom JN,Annema A,Geertsma MF,Langermans JA,Groeneveld PH,de Heer E,van Furth R

MM010 was used in blocking/activating experiment to study the different effects of interleukin 10 on L. monocytogenes proliferation in mice spleens and livers

Tue Aug 01 00:00:00 EDT 2000

Orally administered decoction of Kampo (Japanese herbal) medicine, "Juzen-Taiho-To" modulates cytokine secretion and induces NKT cells in mouse liver.

Immunopharmacology

Matsumoto T,Sakurai MH,Kiyohara H,Yamada H

MM010 was used in ELISA to investigate the effect of Kampo medicine "Juzen-Taiho-To" administered orally for cytokine secretion and NKT cell induction in mouse liver

Tue Feb 01 00:00:00 EST 2000

IL-4 enhances IL-10 gene expression in murine Th2 cells in the absence of TCR engagement.

Journal of immunology (Baltimore, Md. : 1950)

Schmidt-Weber CB,Alexander SI,Henault LE,James L,Lichtman AH

MM010 was used in ELISA to study the effect of interleukin 4 on interleukin 10 expression and its mechanism

Fri Jan 01 00:00:00 EST 1999

Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4.

Journal of immunology (Baltimore, Md. : 1950)

Assenmacher M,Löhning M,Scheffold A,Richter A,Miltenyi S,Schmitz J,Radbruch A

MM010 was used in ELISA to study the mechanism for T cell differentiation

Tue Sep 15 00:00:00 EDT 1998

HindIII liposomes suppress delayed-type hypersensitivity responses in vivo and induce epidermal IL-10 in vitro.

Journal of immunology (Baltimore, Md. : 1950)

Nishigori C,Yarosh D,O'Connor A,Shreedhar VK,Ullrich SE,Cox P,Kripke ML

MM010 was used in ELISA to study the effect of DNA damage on immune responses and its mechanism

Tue Sep 15 00:00:00 EDT 1998

A novel role for TGF-beta and IL-10 in the induction of immune privilege.

Journal of immunology (Baltimore, Md. : 1950)

D'Orazio TJ,Niederkorn JY

MM010 was used in blocking/activating experiment to evaluate the involvement of tumor growth factor beta and interleukin 10 in immune privilege

Sun Mar 01 00:00:00 EST 1998

T cell receptor specificities of Toxoplasma gondii-reactive mouse CD4+ T lymphocytes and Th1 clones.

Medical microbiology and immunology

Reichmann G,Dlugonska H,Fischer HG

MM010 was used in ELISA to investigate the specificities of Toxoplasma gondii-reactive mouse CD4+ T lymphocytes and Th1 clones

Sun Jun 01 00:00:00 EDT 1997

Neutralizing anti-IL-10 antibody upregulates the induction and elicitation of contact hypersensitivity.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Maguire HC,Ketcha KA,Lattime EC

MM010 was used in blocking/activating experiment to investigate the effect of anti-interleukin 10 antibody on contact hypersensitivity

Mon Dec 01 00:00:00 EST 1997

IL-12 is essential for resistance against Yersinia enterocolitica by triggering IFN-gamma production in NK cells and CD4+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Bohn E,Autenrieth IB

MM010 was used in blocking/activating experiment to investigate the importance of interleukin 12 in Yersinia enferocolifica infection in mice

Thu Feb 15 00:00:00 EST 1996

Production of nitric oxide (NO) is not essential for protection against acute Toxoplasma gondii infection in IRF-1-/- mice.

Journal of immunology (Baltimore, Md. : 1950)

Khan IA,Matsuura T,Fonseka S,Kasper LH

MM010 was used in blocking/activating experiment to investigate the role of nitric oxide for the prevention of acute Toxoplasma gondii infection in IRF-1-/- mice

Mon Jan 15 00:00:00 EST 1996

Th1 development of naive CD4+ T cells is inhibited by co-activation with anti-CD4 monoclonal antibodies.

Journal of immunology (Baltimore, Md. : 1950)

Goedert S,Germann T,Hoehn P,Koelsch S,Palm N,Rüde E,Schmitt E

MM010 was used in blocking/activating experiment to investigate the effect of CD4 antibodies on Th1 differentiation

Mon Jul 15 00:00:00 EDT 1996

Local release of IL-10 by transfected mouse mammary adenocarcinoma cells does not suppress but enhances antitumor reaction and elicits a strong cytotoxic lymphocyte and antibody-dependent immune memory.

Journal of immunology (Baltimore, Md. : 1950)

Giovarelli M,Musiani P,Modesti A,Dellabona P,Casorati G,Allione A,Consalvo M,Cavallo F,di Pierro F,De Giovanni C

MM010 was used in ELISA to investigate the effect of interleukin 10 released from transfected mouse mammary adenocarcinoma cells on antitumor reaction, cytotoxic lymphocyte production, and antibody-dependent immune memory

Fri Sep 15 00:00:00 EDT 1995

Systemic cytokine profiles in BALB/c mice immunized with trivalent influenza vaccine containing MF59 oil emulsion and other advanced adjuvants.

Journal of immunology (Baltimore, Md. : 1950)

Valensi JP,Carlson JR,Van Nest GA

MM010 was used in ELISA to investigate the profiles of cytokines in BALB/c mice immunized with trivalent influenza vaccine containing MF59 oil emulsion and other advanced adjuvants

Tue Nov 01 00:00:00 EST 1994

Mechanism involved in the systemic suppression of antigen-presenting cell function by UV irradiation. Keratinocyte-derived IL-10 modulates antigen-presenting cell function of splenic adherent cells.

Journal of immunology (Baltimore, Md. : 1950)

Ullrich SE

MM010 was used in ELISA to investigate the mechanism for the systemic suppression of antigen-presenting cell function by UV irradiation

Fri Apr 01 00:00:00 EST 1994

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1

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Gene Aliases: CSIF; Il-10; Il10

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UniProt ID: (Mouse) P18893

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Entrez Gene ID: (Mouse) 16153

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Function(s)

cytokine activity interleukin-10 receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-10 Monoclonal Antibody (2A5)

IL-10 Antibody (MM010) in Neu

Product Details

MM010

Western Blot (WB)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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