Invitrogen

IL-10 Monoclonal Antibody (2G101H7)

IL-10 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

IL-10 Antibody (ARC9102) in ICC/IF

Immunofluorescence analysis of Interleukin-10 was performed using 70% confluent log phase RAW 264.7 cells treated with LPS (200 ng/mL, 20hrs) followed by PTI treatment (1X, 4hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IL-10 Monoclonal Antibody (2G101H7) (Product # ARC9102) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Ra... View More

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Product Details

ARC9102

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

Assay-dependent

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2G101H7

Immunogen

Recombinant rat IL-10.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2536417

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Plinia trunciflora Extract Administration Prevents HI-Induced Oxidative Stress, Inflammatory Response, Behavioral Impairments, and Tissue Damage in Rats.

Nutrients

Carvalho AVS,Ribeiro RT,Durán-Carabali LE,Martini APR,Hoeper E,Sanches EF,Konrath EL,Dalmaz C,Wajner M,Netto CA

ARC9102 was used in Western Blotting to evaluates the neuroprotective role of the Plinia trunciflora fruit extract (PTE) in neonatal rats submitted to experimental hypoxia-ischemia (HI).

Mon Jan 17 00:00:00 EST 2022

Antioxidant and immunomodulatory activity induced by stevioside in liver damage: In vivo, in vitro and in silico assays.

Life sciences

Casas-Grajales S,Ramos-Tovar E,Chávez-Estrada E,Alvarez-Suarez D,Hernández-Aquino E,Reyes-Gordillo K,Cerda-García-Rojas CM,Camacho J,Tsutsumi V,Lakshman MR,Muriel P

ARC9102 was used in Western Blot to study whether stevioside has beneficial effects in liver injury induced by long-term thioacetamide (TAA) administration and investigate the possible underlying molecular mechanism using in vivo, in vitro and in silico approaches.

Wed May 01 00:00:00 EDT 2019

Naringenin prevents experimental liver fibrosis by blocking TGFβ-Smad3 and JNK-Smad3 pathways.

World journal of gastroenterology

Hernández-Aquino E,Zarco N,Casas-Grajales S,Ramos-Tovar E,Flores-Beltrán RE,Arauz J,Shibayama M,Favari L,Tsutsumi V,Segovia J,Muriel P

ARC9102 was used in Western Blotting to study the molecular mechanisms involved in the hepatoprotective effects of naringenin on carbon tetrachloride-induced liver fibrosis.

Wed Jun 28 00:00:00 EDT 2017

Quercetin reverses experimental cirrhosis by immunomodulation of the proinflammatory and profibrotic processes.

Fundamental & clinical pharmacology

Casas-Grajales S,Vázquez-Flores LF,Ramos-Tovar E,Hernández-Aquino E,Flores-Beltrán RE,Cerda-García-Rojas CM,Camacho J,Shibayama M,Tsutsumi V,Muriel P

Published figure using IL-10 monoclonal antibody (Product # ARC9102) in Western Blot

Fri Dec 01 00:00:00 EST 2017

Extracellular vesicles derived from T regulatory cells suppress T cell proliferation and prolong allograft survival.

Scientific reports

Aiello S,Rocchetta F,Longaretti L,Faravelli S,Todeschini M,Cassis L,Pezzuto F,Tomasoni S,Azzollini N,Mister M,Mele C,Conti S,Breno M,Remuzzi G,Noris M,Benigni A

ARC9102 was used in Western Blotting to propose that DnIKK2-Treg-derived EV could be a tool for manipulating the immune system and for discovering novel potential immunosuppressive molecules in the context of allotransplantation.

Thu Sep 14 00:00:00 EDT 2017

Trolox mitigates fibrosis in a bile duct ligation model.

Fundamental & clinical pharmacology

Galicia-Moreno M,Favari L,Muriel P

ARC9102 was used in western blot to assess the role of trolox in chronic bile duct ligation.

Sat Jun 01 00:00:00 EDT 2013

Antifibrotic and antioxidant effects of N-acetylcysteine in an experimental cholestatic model.

European journal of gastroenterology & hepatology

Galicia-Moreno M,Favari L,Muriel P

Wed Feb 01 00:00:00 EST 2012

Antifibrotic and antioxidant effects of N-acetylcysteine in an experimental cholestatic model.

European journal of gastroenterology & hepatology

Galicia-Moreno M,Favari L,Muriel P

Wed Feb 01 00:00:00 EST 2012

Adaptor protein Shc acts as an immune-regulator for the LPS-stimulated maturation of bone marrow-derived dendritic cells.

BMC immunology

Chen KD,Hsu LW,Goto S,Yeh CW,Nakano T,Lai CY,Chang YC,Hou CH,Wang CC,Cheng YF,Chiu KW,Lin CC,Chen CL

Published figure using IL-10 monoclonal antibody (Product # ARC9102) in Neutralization

Wed May 25 00:00:00 EDT 2011

Adaptor protein Shc acts as an immune-regulator for the LPS-stimulated maturation of bone marrow-derived dendritic cells.

BMC immunology

Chen KD,Hsu LW,Goto S,Yeh CW,Nakano T,Lai CY,Chang YC,Hou CH,Wang CC,Cheng YF,Chiu KW,Lin CC,Chen CL

Published figure using IL-10 monoclonal antibody (Product # ARC9102) in Neutralization

Wed May 25 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1

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Gene Aliases: CSIF; Il-10; Il10; IL10X

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UniProt ID: (Mouse) P18893, (Rat) P29456

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Entrez Gene ID: (Mouse) 16153, (Rat) 25325

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Function(s)

cytokine activity interleukin-10 receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-10 Monoclonal Antibody (2G101H7)

IL-10 Antibody (ARC9102) in ICC/IF

Product Details

ARC9102

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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