Invitrogen

IL-10 Monoclonal Antibody (9D7)

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IL-10 Antibody (M010) in WB

Western blot was performed using Anti-IL-10 Monoclonal Antibody (9D7) (Product # M010) and a 18 kDa band corresponding to Interleukin-10 was observed in Human rec. IL10 as compared to Human rec. IL6 which was used as a negative control. Mouse rec. IL6 was used to test species reactivity. 250 ng of Human rec. IL10 (Lane 1), Human rec. IL6 (Lane 2), Mouse rec. IL10 (Lane 3) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB230... View More

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Product Details

M010

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

ELISA (ELISA)

Assay-dependent

View 22 publications 22 publications

Inhibition Assays (IA)

Assay-dependent

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

9D7

Immunogen

Recombinant human IL-10 (COS cell-derived)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223551

Product Specific Information

M010 targets IL-10 in ELISA, and IA applications and shows reactivity with Human samples.

The M010 immunogen is recombinant human IL-10 (COS cell-derived).

M010 detects IL-10 which has a predicted molecular weight of approximately 19 kDa.

The M010 IL10 antibody (clone 9D7) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M011B (biotinylated conjugate of clone 12G8). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody M010 (clone 9D7) and biotinylated antibody M011B (clone 12G8) have successfully been used in combination with recombinant IL10 protein SIL10 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Methods for in vitro generation of human type 1 regulatory T cells.

Methods in molecular biology (Clifton, N.J.)

Gregori S,Roncarolo MG,Bacchetta R

M010 was used in ELISA to study cytokine production profile of Tr1 cell lines

Wed Feb 02 00:00:00 EST 2011

Molecular and functional characterization of allogantigen-specific anergic T cells suitable for cell therapy.

Haematologica

Bacchetta R,Gregori S,Serafini G,Sartirana C,Schulz U,Zino E,Tomiuk S,Jansen U,Ponzoni M,Paties CT,Fleischhauer K,Roncarolo MG

M010 was used in ELISA to characterize allogantigen-specific anergic T cells

Wed Dec 01 00:00:00 EST 2010

Hepatitis C virus modulates human monocyte-derived dendritic cells.

Journal of viral hepatitis

Eksioglu EA,Bess JR,Zhu H,Xu Y,Dong HJ,Elyar J,Nelson DR,Liu C

M010 was used in ELISA to study the effect of hepatitis C virus on human monocyte-derived dendritic cells

Mon Nov 01 00:00:00 EDT 2010

Type 1 regulatory T cells are associated with persistent split erythroid/lymphoid chimerism after allogeneic hematopoietic stem cell transplantation for thalassemia.

Haematologica

Serafini G,Andreani M,Testi M,Battarra M,Bontadini A,Biral E,Fleischhauer K,Marktel S,Lucarelli G,Roncarolo MG,Bacchetta R

M010 was used in ELISA to investigate the role of type 1 regulatory T cells during persistent split erythroid/lymphoid chimerism after allogeneic hematopoietic stem cell transplantation for thalassemia

Thu Oct 01 00:00:00 EDT 2009

Anti-ribosomal phosphoprotein autoantibody triggers interleukin-10 overproduction via phosphatidylinositol 3-kinase-dependent signalling pathways in lipopolysaccharide-activated macrophages.

Immunology

Lee TP,Leu SJ,Huang JC,Song YC,Jhou RS,Tang SJ,Sun KH

M010 was used in ELISA to investigate the mechanism of interleukin 10 overproduction induced by anti-ribosomal phosphoprotein autoantibody in lipopolysaccharide-activated macrophages

Fri May 01 00:00:00 EDT 2009

Multiplexed Elispot assay.

Journal of immunological methods

Harriman WD,Collarini EJ,Cromer RG,Dutta A,Strandh M,Zhang F,Kauvar LM

M010 was used in ELISA to develop a multiplexed Elispot assay system

Sat Feb 28 00:00:00 EST 2009

Latent membrane protein 1 of EBV activates phosphatidylinositol 3-kinase to induce production of IL-10.

Journal of immunology (Baltimore, Md. : 1950)

Lambert SL,Martinez OM

M010 was used in ELISA to study the induction of interleukin-10 production by EBV latent membrane protein 1

Sat Dec 15 00:00:00 EST 2007

Hepatitis C virus-specific reactivity of CD4+-lymphocytes in children born from HCV-infected women.

Journal of hepatology

Della Bella S,Riva A,Tanzi E,Nicola S,Amendola A,Vecchi L,Nebbia G,Longhi R,Zanetti AR,Villa ML

M010 was used in ELISA to investigate the reactivity of CD4+ lymphocytes specific to hepatitis C virus in children born from HCV-infected women

Thu Sep 01 00:00:00 EDT 2005

Modulation of human dendritic cell phenotype and function by probiotic bacteria.

Gut

Hart AL,Lammers K,Brigidi P,Vitali B,Rizzello F,Gionchetti P,Campieri M,Kamm MA,Knight SC,Stagg AJ

M010 was used in ELISA to study the effects of probiotic bacteria on human dendritic cells

Mon Nov 01 00:00:00 EST 2004

Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.

Rheumatology (Oxford, England)

Schotte H,Schlüter B,Willeke P,Mickholz E,Schorat MA,Domschke W,Gaubitz M

M010 was used in ELISA to study the effect of etanercept treatment on proinflammatory cytokine-secreting peripheral blood mononuclear cells in rheumatoid arthritic patients

Sun Aug 01 00:00:00 EDT 2004

Host factors impacting the innate response in humans to the candidate adjuvants RC529 and monophosphoryl lipid A.

Vaccine

Tiberio L,Fletcher L,Eldridge JH,Duncan DD

M010 was used in ELISA to study the effect of genotypes on the innate response against the candidate adjuvants RC529 and monophosphoryl lipid A

Mon Mar 29 00:00:00 EST 2004

Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients.

Rheumatology international

Solomon S,Masilamani M,Mohanty S,Schwab JE,Boneberg EM,Illges H

M010 was used in ELISA to investigate the generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients

Mon Mar 01 00:00:00 EST 2004

Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients.

Journal of hepatology

Loffreda S,Muratori P,Muratori L,Mele L,Bianchi FB,Lenzi M

M010 was used in ELISA to investigate the augmented production of Th1-type cytokines in HCV-infected cryoglobulinemic patients

Sat Feb 01 00:00:00 EST 2003

Altered maturation of peripheral blood dendritic cells in patients with breast cancer.

British journal of cancer

Della Bella S,Gennaro M,Vaccari M,Ferraris C,Nicola S,Riva A,Clerici M,Greco M,Villa ML

M010 was used in ELISA to characterize the peripheral blood dendritic cell maturation in breast cancer patients

Mon Oct 20 00:00:00 EDT 2003

Measles virus infection of rhesus macaques affects neutrophil expression of IL-12 and IL-10.

Viral immunology

Hoffman SJ,Polack FP,Hauer DA,Griffin DE

M010 was used in flow cytometry and immunocytochemistry to examine IL-12 and IL-10 production by neutrophils isolated from naive or measles virus-infected rhesus macaques.

Mon Jan 05 00:00:00 EST 2004

Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.

Transplantation proceedings

Meloni F,Cascina A,Paschetto E,Marone Bianco A,Morosini M,Pellegrini C,Fietta A,Vitulo P,Pozzi E,Viganò M

M010 was used in ELISA to investigate the effect of immunosuppressive regimens on cytokine expression in lung transplanted patients

Sun Jun 01 00:00:00 EDT 2003

Altered synthesis of interleukin-12 and type 1 and type 2 cytokinesin rhesus macaques during measles and atypical measles.

The Journal of infectious diseases

Polack FP,Hoffman SJ,Moss WJ,Griffin DE

M010 was used in flow cytometry to investigate the role of IL-12 in measles induced immunosuppression.

Tue Jan 01 00:00:00 EST 2002

The neuropeptide bombesin improves IgA-mediated mucosal immunity with preservation of gut interleukin-4 in total parenteral nutrition-fed mice.

Surgery

Zarzaur BL,Wu Y,Fukatsu K,Johnson CD,Kudsk KA

M010 was used in ELISA to study the interleukin levels in the mice with various diets

Tue Jan 01 00:00:00 EST 2002

Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent.

Infection and immunity

McDowell MA,Marovich M,Lira R,Braun M,Sacks D

M010 was used in ELISA to investigate the mechanism for the effect of Leishmania on interleukin 12 p70 secretion

Thu Aug 01 00:00:00 EDT 2002

Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.

Experimental lung research

Suwa T,Hogg JC,Vincent R,Mukae H,Fujii T,van Eeden SF

M010 was used in ELISA to test if factors released from human alveolar macrophages exposed to air pollution particles accelerate the maturation of granulocyte precursors

Tue Jul 09 00:00:00 EDT 2002

Adaptive immune responses of patients with asthma to the attachment (G) glycoprotein of respiratory synctial virus.

The Journal of infectious diseases

Hancock GE,Scheuer CA,Sierzega R,Pryharski KS,McBride JT,Watelet LF,Tebbey PW,Smith JD

M010 was used in ELISA to study the effect of the attachment (G) glycoprotein from respiratory synctial virus on wheezing

Sat Dec 15 00:00:00 EST 2001

Autoregulation of human monocyte-derived dendritic cell maturation and IL-12 production by cyclooxygenase-2-mediated prostanoid production.

Journal of immunology (Baltimore, Md. : 1950)

Whittaker DS,Bahjat KS,Moldawer LL,Clare-Salzler MJ

M010 was used in ELISA to study the mechanism for the effect of prostaglandins on the maturation and function of monocyte-derived dendritic cells

Sun Oct 15 00:00:00 EDT 2000

Nitric oxide in Tanzanian children with malaria: inverse relationship between malaria severity and nitric oxide production/nitric oxide synthase type 2 expression.

The Journal of experimental medicine

Anstey NM,Weinberg JB,Hassanali MY,Mwaikambo ED,Manyenga D,Misukonis MA,Arnelle DR,Hollis D,McDonald MI,Granger DL

M010 was used in ELISA to measure the interleukin-10 and interleukin-4 level in Tanzanian children with malaria

Thu Aug 01 00:00:00 EDT 1996

Contribution of IL-1, CD14, and CD13 in the increased IL-6 production induced by in vitro monocyte-synoviocyte interactions.

Journal of immunology (Baltimore, Md. : 1950)

Chomarat P,Rissoan MC,Pin JJ,Banchereau J,Miossec P

M010 was used in ELISA to investigate the effect of interleukin 1, CD14, and CD13 on the expression of interleukin 6 produced by monocytes and synoviocytes in vitro

Sun Oct 01 00:00:00 EDT 1995

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

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Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

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UniProt ID: (Human) P22301

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Entrez Gene ID: (Human) 3586

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Function(s)

cytokine activity interleukin-10 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-10 Monoclonal Antibody (9D7)

IL-10 Antibody (M010) in WB

Product Details

M010

Western Blot (WB)

ELISA (ELISA)

Inhibition Assays (IA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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