Invitrogen

IL-10 Monoclonal Antibody (JES5-2A5), Biotin, eBioscience™

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IL-10 Antibody (13-7102-81) in Neu

Disease models & mechanisms 2016 - Published figure using IL-10 monoclonal antibody (Product # 13-7102-81) in Neutralization

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Product Details

13-7102-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

0.5-2 µg/mL

View 6 publications 6 publications

Neutralization (Neu)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES5-2A5

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466918

Product Specific Information

Description: The JES5-2A5 antibody reacts with mouse interleukin-10 (IL-10). The JES5-2A5 antibody is a neutralizing antibody. Mouse IL-10 is an 18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Applications Reported: The JES5-2A5 antibody has been reported for use in ELISA, ELISPOT, neutralization of IL-10 bioactivity, and intracellular staining for flow cytometric analysis.

Applications Tested: The JES5-2A5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse Interleukin-10 (IL-10) in combination with the purified JES5-16E3 (14-7101) antibody for capture and recombinant mouse IL-10 (14-8101) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Analysis of the Trichuris suis excretory/secretory proteins as a function of life cycle stage and their immunomodulatory properties.

Scientific reports

Leroux LP,Nasr M,Valanparambil R,Tam M,Rosa BA,Siciliani E,Hill DE,Zarlenga DS,Jaramillo M,Weinstock JV,Geary TG,Stevenson MM,Urban JF,Mitreva M,Jardim A

13-7102 was used in an ELISA assay to analyse the Trichuris suis excretory/secretory proteins as a function of life cycle stage and their immunomodulatory properties.

Mon Oct 29 00:00:00 EDT 2018

Inhibition of Inflammatory Gene Transcription by IL-10 Is Associated with Rapid Suppression of Lipopolysaccharide-Induced Enhancer Activation.

Journal of immunology (Baltimore, Md. : 1950)

Conaway EA,de Oliveira DC,McInnis CM,Snapper SB,Horwitz BH

13-7102 was used in an ELISA assay to demonstrate that inhibition of transcription is the primary mechanism for IL-10-mediated suppression in LPS-stimulated macrophages and that inhibited genes can be divided into two clusters.

Sat Apr 01 00:00:00 EDT 2017

IFNGR1 signaling is associated with adverse pregnancy outcomes during infection with malaria parasites.

PloS one

Niikura M,Inoue SI,Mineo S,Asahi H,Kobayashi F

13-7102 was used in an ELISA assay to explore the role of IFN-γ receptor 1 signalling in placental pathology during severe malaria using luciferase-expressing rodent malaria parasites, P. berghei NK65.

Wed Nov 29 00:00:00 EST 2017

Autocrine IL-10 activation of the STAT3 pathway is required for pathological macrophage differentiation in polycystic kidney disease.

Disease models & mechanisms

Peda JD,Salah SM,Wallace DP,Fields PE,Grantham CJ,Fields TA,Swenson-Fields KI

Published figure using IL-10 monoclonal antibody (Product # 13-7102-81) in Neutralization

Thu Sep 01 00:00:00 EDT 2016

Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment.

Nature communications

Ulland TK,Jain N,Hornick EE,Elliott EI,Clay GM,Sadler JJ,Mills KA,Janowski AM,Volk AP,Wang K,Legge KL,Gakhar L,Bourdi M,Ferguson PJ,Wilson ME,Cassel SL,Sutterwala FS

13-7102 was used in an ELISA assay to demonstrate that C57BL/6J mice have a functional defect in NLRP12, which is required by macrophages for effective neutrophil recruitment to inflammatory sites.

Tue Oct 25 00:00:00 EDT 2016

The sst1 resistance locus regulates evasion of type I interferon signaling by Chlamydia pneumoniae as a disease tolerance mechanism.

PLoS pathogens

He X,Berland R,Mekasha S,Christensen TG,Alroy J,Kramnik I,Ingalls RR

Published figure using IL-10 monoclonal antibody (Product # 13-7102-81) in Neutralization

Mon Mar 17 00:00:00 EDT 2014

Neisseria gonorrhoeae induces a tolerogenic phenotype in macrophages to modulate host immunity.

Mediators of inflammation

Escobar A,Candia E,Reyes-Cerpa S,Villegas-Valdes B,Neira T,Lopez M,Maisey K,Tempio F,Ríos M,Acuña-Castillo C,Imarai M

13-7102 was used in an ELISA assay to determine whether N. gonorrhoeae directly conditions the phenotype of RAW 264.7 murine macrophage cell line and its response.

Thu Jun 05 00:00:00 EDT 2014

Type I IFN receptor and the B cell antigen receptor regulate TLR7 responses via distinct molecular mechanisms.

Journal of immunology (Baltimore, Md. : 1950)

Poovassery JS,Bishop GA

13-7102 was used in an ELISA assay to investigate the mechanisms that regulate TLR7 signals in the innate immune response, showing that Type I IFN receptor and the B cell antigen receptors are involved.

Wed Aug 15 00:00:00 EDT 2012

Osteoclast activated FoxP3+ CD8+ T-cells suppress bone resorption in vitro.

PloS one

Buchwald ZS,Kiesel JR,DiPaolo R,Pagadala MS,Aurora R

13-7102 was used in Flow cytometry/Cell sorting to demonstrate the effects of activated CD8 T cell-produced cytokines on osteoclast activity.

Thu Nov 08 00:00:00 EST 2012

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1

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Gene Aliases: CSIF; Il-10; Il10

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UniProt ID: (Mouse) P18893

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Entrez Gene ID: (Mouse) 16153

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Function(s)

cytokine activity interleukin-10 receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-10 Monoclonal Antibody (JES5-2A5), Biotin, eBioscience™

IL-10 Antibody (13-7102-81) in Neu

Product Details

13-7102-81

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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