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IL-11 Antibody (500-P01BT-25UG) in WB
Western Blot: To detect Human IL-11 by Western Blot analysis IL-11 Polyclonal Antibody, Biotin (Product # 500-P01BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human IL-11 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P01BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-11
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929133
Product Specific Information
AA Sequence of recombinant protein: MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-11. Anti-Human IL-11-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human IL-11 by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-11 (500-P01) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human IL-11
Western Blot: To detect Human IL-11 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human IL-11 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P01BT-1MG will be provided as 2 x 500 µg
Target Information
Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adipogenesis inhibitory factor; AGIF; il 11; IL-11; IL-11ra; IL10RA; IL11 inhibitor; ILN; Interleukin; Interleukin-11; Interleukin11; MGC2146; oprelvekin
Gene Aliases: AGIF; IL-11; IL11
UniProt ID: (Human) P20809
Entrez Gene ID: (Human) 3589
cell-cell signaling
positive regulation of cell proliferation
B cell differentiation
megakaryocyte differentiation
positive regulation of peptidyl-serine phosphorylation
positive regulation of MAPK cascade
fat cell differentiation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of hormone secretion
positive regulation of peptidyl-tyrosine phosphorylation
regulation of complement-dependent cytotoxicity
negative regulation of endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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