Invitrogen

IL-12/IL-23 p40 Monoclonal Antibody (116224)

View all (44) IL-12/IL-23 p40 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-23768

Applications
Tested Dilution
Publications

ELISA (ELISA)

0.5-2 µg/mL (Detection), 2-8 µg/mL (Capture)
-
Product Specifications

Species Reactivity

Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

116224

Immunogen

CHO-derived recombinant porcine IL-12 p70 heterodimer

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2607645

Product Specific Information

This antibody detects the p40 subunit of porcine IL-12/IL-23 in ELISAs and Western blots. In ELISAs, this antibody shows 25-35% cross-reactivity with recombinant human IL-12 and IL-23 and no cross-reactivity with recombinant mouse IL-12 or IL-23.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 subunit p35; IL-12 subunit p40; IL-12A; IL-12B; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12 p40; IL23; il23; IL23 p41; ILN; Interleukin; Interleukin 12; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 23, alpha subunit p19; interleukin-12 p35 subunit; interleukin-12 p40 subunit; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; interleukin-23 p19 subunit; interleukin-23 p40 subunit; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; Interleukin12; RP23-388G23.1

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Gene Aliases: IL12A; IL12B; IL23A; il23p19; SGRF

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UniProt ID: (Pig) Q29053, (Pig) Q28938, (Pig) Q9N2H9

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Entrez Gene ID: (Pig) 397053, (Pig) 397076, (Pig) 100155546

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Function(s)
cytokine activity interleukin-12 receptor binding growth factor activity interleukin-12 beta subunit binding interleukin-27 binding protein heterodimerization activity identical protein binding interleukin-23 receptor binding cytokine interleukin superfamily intercellular signal molecule transmembrane signal receptor
Process(es)
positive regulation of T cell mediated cytotoxicity positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target cell cycle arrest cell proliferation response to UV-B cell migration negative regulation of interleukin-17 production positive regulation of interferon-gamma production positive regulation of natural killer cell activation positive regulation of smooth muscle cell apoptotic process T-helper 1 cell activation T-helper 1 cell cytokine production positive regulation of T cell proliferation positive regulation of tyrosine phosphorylation of STAT protein defense response to protozoan positive regulation of T cell differentiation negative regulation of smooth muscle cell proliferation negative regulation of protein secretion defense response to Gram-positive bacterium positive regulation of NK T cell activation cellular response to lipopolysaccharide extrinsic apoptotic signaling pathway cellular response to virus negative regulation of vascular endothelial growth factor signaling pathway negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis positive regulation of dendritic cell chemotaxis positive regulation of defense response to virus by host positive regulation of T-helper 1 type immune response negative regulation of inflammatory response to antigenic stimulus positive regulation of activation of Janus kinase activity natural killer cell activation negative regulation of interleukin-10 production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-17 production positive regulation of tumor necrosis factor production positive regulation of natural killer cell proliferation T-helper cell differentiation positive regulation of activated T cell proliferation positive regulation of osteoclast differentiation defense response to Gram-negative bacterium positive regulation of NK T cell proliferation defense response to virus cellular response to interferon-gamma inflammatory response signal transduction T cell proliferation positive regulation of memory T cell differentiation innate immune response positive regulation of transcription from RNA polymerase II promoter tissue remodeling positive regulation of neutrophil chemotaxis
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