Invitrogen

IL-12/IL-23 p40 Monoclonal Antibody (C15.6), eBioscience™

Product Details

14-7125-81

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

ELISA (ELISA)

0.25-1 µg/mL

View 6 publications 6 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

C15.6

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468444

Product Specific Information

Description: The C15.6 antibody reacts with the p40 subunit of mouse interleukin-12 and IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated B cells and macrophages. IL-23 is a structurally and biologically-related cytokine comprised of the same p40 subunit and a p19 subunit.

For optimal detection of total IL-12 p40 (monomer, heterodimer, homodimer) by ELISA, a combination of purified C15.6 antibody for capture and biotinylated C17.8 antibody for detection is recommended. Fluorochrome conjugated C15.6 antibody is effective for staining intracellular IL-12.

Applications Reported: The C15.6 antibody has been reported for use in ELISA.

Applications Tested: The C15.6 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IL-12 (p40 monomer or p40 dimerized with p35) or mouse IL-23 (p40 dimerized with p19) in combination with the biotin C17.8 (13-7123) antibody for detection and recombinant mouse IL-12 p70 (14-8121) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25-1 µg/mL. A standard curve consisting of doubling dilutions of the recombinant IL-12 p70 standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Absence of GdX/UBL4A Protects against Inflammatory Diseases by Regulating NF-кB Signaling in Macrophages and Dendritic Cells.

Theranostics

Liu C,Zhou Y,Li M,Wang Y,Yang L,Yang S,Feng Y,Wang Y,Wang Y,Ren F,Li J,Dong Z,Chin YE,Fu X,Wu L,Chang Z

14-7125-81 was used in ELISA to investigate whether ubiquitin-like protein 4A has a role during the innate immune response.

Fri Jan 31 00:00:00 EST 2020

Cysteine-Reactive Free ISG15 Generates IL-1β-Producing CD8α⁺ Dendritic Cells at the Site of Infection.

Journal of immunology (Baltimore, Md. : 1950)

Napolitano A,van der Veen AG,Bunyan M,Borg A,Frith D,Howell S,Kjaer S,Beling A,Snijders AP,Knobeloch KP,Frickel EM

14-7125 was used in ELISA to present Toxoplasma infection as a novel in vivo murine model to study the immunomodulatory properties of free ISG15 and uniquely link it to IL-1Β production by CD8α+ dendritic cells driven by two cysteines in the hinge region of the protein.

Sun Jul 15 00:00:00 EDT 2018

Intestinal bacteria induce TSLP to promote mutualistic T-cell responses.

Mucosal immunology

Mosconi I,Geuking MB,Zaiss MM,Massacand JC,Aschwanden C,Kwong Chung CK,McCoy KD,Harris NL

14-7125 was used in an ELISA assay to show that steady-state thymic stromal lymphopoietin expression requires intestinal bacteria and has an important role in limiting the expansion T helper cells in the colon.

Fri Nov 01 00:00:00 EDT 2013

Nrf2 is essential for cholesterol crystal-induced inflammasome activation and exacerbation of atherosclerosis.

European journal of immunology

Freigang S,Ampenberger F,Spohn G,Heer S,Shamshiev AT,Kisielow J,Hersberger M,Yamamoto M,Bachmann MF,Kopf M

14-7125 was used in an ELISA assay to identify NF-E2-related 2 as an essential positive regulator of inflammasome activation and IL-1-mediated vascular inflammation.

Fri Jul 01 00:00:00 EDT 2011

The ADAMTS1 protease gene is required for mammary tumor growth and metastasis.

The American journal of pathology

Ricciardelli C,Frewin KM,Tan Ide A,Williams ED,Opeskin K,Pritchard MA,Ingman WV,Russell DL

14-7125 was used in Immunohistochemistry to demonstrate that in vivo A disintegrin and metalloprotease with thrombospondin motifs protein 1 may promote mammary tumour growth and progression to metastasis in the PyMT model.

Thu Dec 01 00:00:00 EST 2011

Macrophage-mediated but gamma interferon-independent innate immune responses control the primary wave of Plasmodium yoelii parasitemia.

Infection and immunity

Couper KN,Blount DG,Hafalla JC,van Rooijen N,de Souza JB,Riley EM

14-7125 was used in an ELISA assay to discern the factors that control the primary wave of plasmodium yoelii parasitaemia.

Sat Dec 01 00:00:00 EST 2007

Host cell Ca2+ and protein kinase C regulate innate recognition of Toxoplasma gondii.

Journal of cell science

Masek KS,Fiore J,Leitges M,Yan SF,Freedman BD,Hunter CA

14-7125 was used in an ELISA assay to investigate the role of Ca2+ in Toxoplasma gondii infection.

Wed Nov 01 00:00:00 EST 2006

Repeated topical challenge with chemical antigen elicits sustained dermatitis in NC/Nga mice in specific-pathogen-free condition.

The Journal of investigative dermatology

Tomimori Y,Tanaka Y,Goto M,Fukuda Y

14-7125-81 was used in Immunohistochemistry to suggest that IL-12 plays a role in the persistent skin reaction in NC/Nga mice. The action of IL-12 might be mediated by the decrease in IL-4 production.

Sat Jan 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 subunit p35; IL-12 subunit p40; IL-12A; IL-12B; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12 p40; IL23; il23; IL23 p41; ILN; Interleukin; Interleukin 12; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; Interleukin12; RP23-388G23.1

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Gene Aliases: Il-12a; Il-12b; IL-12p35; Il-12p40; IL-23; Il12a; Il12b; Il12p40; Il23a; Ll12a; p19; p35; p40

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UniProt ID: (Mouse) P43431, (Mouse) P43432, (Mouse) Q9EQ14

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Entrez Gene ID: (Mouse) 16159, (Mouse) 16160, (Mouse) 83430

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Function(s)

cytokine activity protein binding interleukin-23 receptor binding cytokine interleukin superfamily intercellular signal molecule transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-12/IL-23 p40 Monoclonal Antibody (C15.6), eBioscience™

Product Details

14-7125-81

Immunohistochemistry (IHC)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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