Invitrogen

IL-12/IL-23 p40 Monoclonal Antibody (C8.6)

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View all (44) IL-12/IL-23 p40 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

M121

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
View 7 publications 7 publications

Neutralization (Neu)

-
View 2 publications 2 publications

Inhibition Assays (IA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

C8.6

Immunogen

COS cell-derived recombinant human IL-12, p40 subunit

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223554

Product Specific Information

This antibody is specific for natural IL-12 and recognizes the p70 heterodimer as well as the p40 subunit (monomers and homodimers). It will not cross-react with the p35 subunit.

This is a low-endotoxin formulation.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL-12A; IL-12B; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12 p40; IL23; IL12, subunit p40; il23; IL23 p41; IL35 subunit; ILN; Interleukin; Interleukin 12; interleukin 12, p35; interleukin 12, p40; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-12 alpha chain; interleukin-12 beta chain; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; Interleukin12; JKA3 induced upon T-cell activation; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF1; NKSF2; RP23-388G23.1

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Gene Aliases: CLMF; CLMF2; IL-12A; IL-12B; IL-23; IL-23A; IL12A; IL12B; IL23A; IL23P19; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P19; P35; SGRF; UNQ2498/PRO5798

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UniProt ID: (Human) P29459, (Human) P29460, (Human) Q9NPF7

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Entrez Gene ID: (Human) 3592, (Human) 3593, (Human) 51561

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Function(s)
cytokine activity interleukin-12 receptor binding protein binding growth factor activity interleukin-12 beta subunit binding interleukin-27 binding protein heterodimerization activity cytokine receptor activity interleukin-12 alpha subunit binding identical protein binding protein homodimerization activity interleukin-23 receptor binding transmembrane signal receptor cytokine immunoglobulin interleukin superfamily intercellular signal molecule
Process(es)
positive regulation of T cell mediated cytotoxicity positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target immune response cell cycle arrest response to virus response to UV-B cell migration response to lipopolysaccharide negative regulation of interleukin-17 production positive regulation of interferon-gamma production positive regulation of natural killer cell activation positive regulation of mononuclear cell proliferation positive regulation of smooth muscle cell apoptotic process positive regulation of T cell proliferation positive regulation of tyrosine phosphorylation of Stat4 protein defense response to protozoan positive regulation of T cell differentiation positive regulation of cell adhesion positive regulation of natural killer cell mediated cytotoxicity negative regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation defense response to Gram-positive bacterium positive regulation of NK T cell activation cellular response to lipopolysaccharide extrinsic apoptotic signaling pathway cellular response to virus positive regulation of dendritic cell chemotaxis positive regulation of defense response to virus by host natural killer cell activation involved in immune response positive regulation of T-helper 1 type immune response negative regulation of inflammatory response to antigenic stimulus positive regulation of activation of JAK2 kinase activity cytokine-mediated signaling pathway sensory perception of pain sexual reproduction natural killer cell activation negative regulation of interleukin-10 production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-17 production positive regulation of tumor necrosis factor production positive regulation of natural killer cell proliferation positive regulation of tissue remodeling regulation of cytokine biosynthetic process T-helper 1 type immune response T-helper cell differentiation interferon-gamma biosynthetic process positive regulation of activated T cell proliferation positive regulation of NF-kappaB import into nucleus regulation of tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat5 protein positive regulation of memory T cell differentiation negative regulation of growth of symbiont in host positive regulation of interferon-gamma biosynthetic process positive regulation of osteoclast differentiation positive regulation of inflammatory response defense response to Gram-negative bacterium positive regulation of NK T cell proliferation defense response to virus cellular response to interferon-gamma positive regulation of T-helper 17 type immune response positive regulation of T-helper 17 cell lineage commitment inflammatory response T cell proliferation innate immune response positive regulation of transcription from RNA polymerase II promoter tissue remodeling positive regulation of neutrophil chemotaxis
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