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Invitrogen
IL-12 p35 Monoclonal Antibody (B-T21 (BT21)), eBioscience™
Product Details
14-7128-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
B-T21 (BT21)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_468448
Product Specific Information
Description: The B-T21 antibody reacts with the p35 subunit of human interleukin-12 (IL-12). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated B cells. For optimal detection of human IL-12 p70 by ELISA, a combination of purified B-T21 antibody for capture and biotinylated C8.6 antibody for detection is recommended.
Applications Reported: The B-T21 antibody has been reported for use as capture antibody in an IL-12 p70 sandwich ELISA.
Applications Tested: The B-T21 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-12 p70 in combination with the biotinylated C8.6 (13-7129) antibody for detection and recombinant human IL-12 p70 (14-8129) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily. Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities. Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells. IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells. It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein. The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A. Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CLMF p35; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; il 12; IL-12 subunit p35; IL-12, subunit p35; IL-12A; IL12p35; IL35 subunit; ILN; Interleukin; Interleukin 12; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain; Interleukin-12 subunit alpha; Interleukin12; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1
Gene Aliases: CLMF; IL-12A; IL12A; NFSK; NKSF1; P35
UniProt ID: (Human) P29459
Entrez Gene ID: (Human) 3592
positive regulation of T cell mediated cytotoxicity
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
immune response
cell cycle arrest
response to virus
response to UV-B
cell migration
response to lipopolysaccharide
negative regulation of interleukin-17 production
positive regulation of interferon-gamma production
positive regulation of natural killer cell activation
positive regulation of mononuclear cell proliferation
positive regulation of smooth muscle cell apoptotic process
positive regulation of T cell proliferation
positive regulation of tyrosine phosphorylation of Stat4 protein
defense response to protozoan
positive regulation of T cell differentiation
positive regulation of cell adhesion
positive regulation of natural killer cell mediated cytotoxicity
negative regulation of smooth muscle cell proliferation
positive regulation of lymphocyte proliferation
defense response to Gram-positive bacterium
positive regulation of NK T cell activation
cellular response to lipopolysaccharide
extrinsic apoptotic signaling pathway
cellular response to virus
positive regulation of dendritic cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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