Invitrogen

IL-12 p40 Polyclonal Antibody

View all (32) IL-12 p40 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-47197

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1 µg/mL
-

Neutralization (Neu)

0.05-0.25 µg/mL
-
Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Sf 21-derived recombinant rat IL-12 p40: Met23-Ser335; p35: Arg23-Ser215

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610678

Product Specific Information

In direct ELISAs, approximatley 50% cross-reactivity with recombinant mouse IL-12 is observed, 5% cross-reactivity with recombinant human IL-12 is observed, and less than 10% cross-reactivity with recombinant feline IL-12 and recombinant canine IL-12 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.30 EU per 1 µg of the antibody by the LAL method.

Target Information

IL12B (IL-12 p40) encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kDa cytokine receptor like subunit encoded by this gene, and a 35 kDa subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: il 12; IL12 p40; IL12A; ILN; Interleukin; Interleukin 12; interleukin-12 p40; interleukin-12 subunit beta; Interleukin12; RP23-388G23.1

View more View less

Gene Aliases: Il12

View more View less

Entrez Gene ID: (Rat) 64546

View more View less

Function(s)
cytokine receptor activity cytokine activity cytokine receptor binding growth factor activity interleukin-12 alpha subunit binding identical protein binding protein homodimerization activity interleukin-23 receptor binding protein heterodimerization activity transmembrane signal receptor
Process(es)
positive regulation of T cell mediated cytotoxicity positive regulation of defense response to virus by host natural killer cell activation involved in immune response positive regulation of T-helper 1 type immune response positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target negative regulation of inflammatory response to antigenic stimulus cell cycle arrest cell surface receptor signaling pathway response to UV-B positive regulation of activation of JAK2 kinase activity cell migration cytokine-mediated signaling pathway sensory perception of pain natural killer cell activation negative regulation of interleukin-10 production negative regulation of interleukin-17 production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-17 production positive regulation of tumor necrosis factor production positive regulation of natural killer cell activation positive regulation of natural killer cell proliferation positive regulation of mononuclear cell proliferation positive regulation of smooth muscle cell apoptotic process T-helper cell differentiation positive regulation of T cell proliferation positive regulation of activated T cell proliferation regulation of tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat4 protein positive regulation of tyrosine phosphorylation of Stat5 protein defense response to protozoan negative regulation of growth of symbiont in host positive regulation of osteoclast differentiation positive regulation of cell adhesion negative regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation defense response to Gram-negative bacterium positive regulation of NK T cell activation positive regulation of NK T cell proliferation defense response to virus cellular response to lipopolysaccharide cellular response to interferon-gamma
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support