Invitrogen

IL-12 p40 Recombinant Rabbit Monoclonal Antibody (21 H19L14)

View all (32) IL-12 p40 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

701233

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-2 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

21 H19L14

Immunogen

Recombinant protein corresponding to amino acids 23-172 of human IL-12B p40.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532433

Product Specific Information

This antibody is predicted to react with non-human primate, mouse and rat based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

IL12B (IL-12 p40) encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kDa cytokine receptor like subunit encoded by this gene, and a 35 kDa subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CLMF p40; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL-12 subunit p40; IL-12B; IL12 p40; IL12, subunit p40; IL12A; ILN; Interleukin; Interleukin 12; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 beta chain; Interleukin-12 subunit beta; Interleukin12; natural killer cell stimulatory factor, 40 kD subunit; NK cell stimulatory factor chain 2; NKSF2; RP23-388G23.1

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Gene Aliases: CLMF; CLMF2; IL-12B; IL12B; IMD28; IMD29; NKSF; NKSF2

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UniProt ID: (Human) P29460

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Entrez Gene ID: (Human) 3593

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Function(s)
cytokine receptor activity cytokine activity interleukin-12 receptor binding protein binding growth factor activity interleukin-12 alpha subunit binding identical protein binding protein homodimerization activity interleukin-23 receptor binding protein heterodimerization activity transmembrane signal receptor
Process(es)
positive regulation of T cell mediated cytotoxicity positive regulation of defense response to virus by host natural killer cell activation involved in immune response positive regulation of T-helper 1 type immune response positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target negative regulation of inflammatory response to antigenic stimulus cell cycle arrest response to UV-B positive regulation of activation of JAK2 kinase activity cell migration cytokine-mediated signaling pathway sensory perception of pain sexual reproduction natural killer cell activation negative regulation of interleukin-10 production negative regulation of interleukin-17 production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-17 production positive regulation of tumor necrosis factor production positive regulation of natural killer cell activation positive regulation of natural killer cell proliferation positive regulation of mononuclear cell proliferation positive regulation of tissue remodeling positive regulation of smooth muscle cell apoptotic process regulation of cytokine biosynthetic process T-helper 1 type immune response T-helper cell differentiation interferon-gamma biosynthetic process positive regulation of T cell proliferation positive regulation of activated T cell proliferation positive regulation of NF-kappaB import into nucleus regulation of tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat4 protein positive regulation of tyrosine phosphorylation of Stat5 protein defense response to protozoan positive regulation of memory T cell differentiation negative regulation of growth of symbiont in host positive regulation of interferon-gamma biosynthetic process positive regulation of osteoclast differentiation positive regulation of cell adhesion negative regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation positive regulation of inflammatory response defense response to Gram-negative bacterium positive regulation of NK T cell activation positive regulation of NK T cell proliferation defense response to virus cellular response to lipopolysaccharide cellular response to interferon-gamma positive regulation of T-helper 17 type immune response positive regulation of T-helper 17 cell lineage commitment
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